Research output: Contribution to journal › Article › Academic › peer-review
Transhepatic bile acid kinetics in pigs and humans. / Eggink, Hannah M.; van Nierop, F. Samuel; Schooneman, Marieke G. et al.
In: Clinical nutrition (Edinburgh, Scotland), Vol. 37, No. 4, 2018, p. 1406-1414.Research output: Contribution to journal › Article › Academic › peer-review
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TY - JOUR
T1 - Transhepatic bile acid kinetics in pigs and humans
AU - Eggink, Hannah M.
AU - van Nierop, F. Samuel
AU - Schooneman, Marieke G.
AU - Boelen, Anita
AU - Kalsbeek, Andries
AU - Koehorst, Martijn
AU - ten Have, Gabriella A. M.
AU - de Brauw, L. Maurits
AU - Groen, Albert K.
AU - Romijn, Johannes A.
AU - Deutz, Nicolaas E. P.
AU - Soeters, Maarten R.
PY - 2018
Y1 - 2018
N2 - Bile acids (BAs) play a key role in lipid uptake and metabolic signalling in different organs including gut, liver, muscle and brown adipose tissue. Portal and peripheral plasma BA concentrations increase after a meal. However, the exact kinetics of postprandial BA metabolism have never been described in great detail. We used a conscious porcine model to investigate postprandial plasma concentrations and transorgan fluxes of BAs, glucose and insulin using the para-aminohippuric acid dilution method. Eleven pigs with intravascular catheters received a standard mixed-meal while blood was sampled from different veins such as the portal vein, abdominal aorta and hepatic vein. To translate the data to humans, fasted venous and portal blood was sampled from non-diabetic obese patients during gastric by-pass surgery. The majority of the plasma bile acid pool and postprandial response consisted of glycine-conjugated forms of primary bile acids. Conjugated bile acids were more efficiently cleared by the liver than unconjugated forms. The timing and size of the postprandial response showed large interindividual variability for bile acids compared to glucose and insulin. The liver selectively extracts most BAs and BAs with highest affinity for the most important metabolic BA receptor, TGR5, are typically low in both porcine and human peripheral circulation. Our findings raise questions about the magnitude of a peripheral TGR5 signal and its ultimate clinical application
AB - Bile acids (BAs) play a key role in lipid uptake and metabolic signalling in different organs including gut, liver, muscle and brown adipose tissue. Portal and peripheral plasma BA concentrations increase after a meal. However, the exact kinetics of postprandial BA metabolism have never been described in great detail. We used a conscious porcine model to investigate postprandial plasma concentrations and transorgan fluxes of BAs, glucose and insulin using the para-aminohippuric acid dilution method. Eleven pigs with intravascular catheters received a standard mixed-meal while blood was sampled from different veins such as the portal vein, abdominal aorta and hepatic vein. To translate the data to humans, fasted venous and portal blood was sampled from non-diabetic obese patients during gastric by-pass surgery. The majority of the plasma bile acid pool and postprandial response consisted of glycine-conjugated forms of primary bile acids. Conjugated bile acids were more efficiently cleared by the liver than unconjugated forms. The timing and size of the postprandial response showed large interindividual variability for bile acids compared to glucose and insulin. The liver selectively extracts most BAs and BAs with highest affinity for the most important metabolic BA receptor, TGR5, are typically low in both porcine and human peripheral circulation. Our findings raise questions about the magnitude of a peripheral TGR5 signal and its ultimate clinical application
U2 - 10.1016/j.clnu.2017.06.015
DO - 10.1016/j.clnu.2017.06.015
M3 - Article
C2 - 28669667
VL - 37
SP - 1406
EP - 1414
JO - Clinical nutrition (Edinburgh, Scotland)
JF - Clinical nutrition (Edinburgh, Scotland)
SN - 0261-5614
IS - 4
ER -
ID: 3943044