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Thrombocidin-1-derived antimicrobial peptide TC19 combats superficial multi-drug resistant bacterial wound infections. / Riool, Martijn; de Breij, Anna; Kwakman, Paulus H. S. et al.

In: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, Vol. 1862, No. 8, 183282, 01.08.2020.

Research output: Contribution to journal › Article › Academic › peer-review

Harvard

Riool, M, de Breij, A, Kwakman, PHS, Schonkeren-Ravensbergen, E, de Boer, L, Cordfunke, RA, Malanovic, N, Drijfhout, JW, Nibbering, PH & Zaat, SAJ 2020, 'Thrombocidin-1-derived antimicrobial peptide TC19 combats superficial multi-drug resistant bacterial wound infections', BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, vol. 1862, no. 8, 183282. https://doi.org/10.1016/j.bbamem.2020.183282

APA

Riool, M., de Breij, A., Kwakman, P. H. S., Schonkeren-Ravensbergen, E., de Boer, L., Cordfunke, R. A., Malanovic, N., Drijfhout, J. W., Nibbering, P. H., & Zaat, S. A. J. (2020). Thrombocidin-1-derived antimicrobial peptide TC19 combats superficial multi-drug resistant bacterial wound infections. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1862(8), [183282]. https://doi.org/10.1016/j.bbamem.2020.183282

Vancouver

Riool M, de Breij A, Kwakman PHS, Schonkeren-Ravensbergen E, de Boer L, Cordfunke RA et al. Thrombocidin-1-derived antimicrobial peptide TC19 combats superficial multi-drug resistant bacterial wound infections. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES. 2020 Aug 1;1862(8):183282. Epub 2020. doi: 10.1016/j.bbamem.2020.183282

Author

Riool, Martijn ; de Breij, Anna ; Kwakman, Paulus H. S. et al. / Thrombocidin-1-derived antimicrobial peptide TC19 combats superficial multi-drug resistant bacterial wound infections. In: BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES. 2020 ; Vol. 1862, No. 8.

BibTeX

@article{8f032456589449359b7340decc58d513,
title = "Thrombocidin-1-derived antimicrobial peptide TC19 combats superficial multi-drug resistant bacterial wound infections",
abstract = "Antimicrobial peptides are considered promising candidates for the development of novel antimicrobial agents to combat infections by multi-drug-resistant (MDR) bacteria. Here, we describe the identification and characterization of the synthetic peptide TC19, derived from the human thrombocidin-1-derived peptide L3. Biophysical experiments into the interaction between TC19 and mimics of human and bacterial plasma membranes demonstrated that the peptide is highly selective for bacterial membranes. In agreement, TC19 combined low cytotoxicity towards human fibroblasts with efficient and rapid killing in human plasma of MDR strains of several bacterial species of the ESKAPE panel. In addition, TC19 induced minor resistance in vitro, neutralized pro-inflammatory activity of bacterial cell envelope components while displaying slight chemotactic activity for human neutrophils. Importantly, topical application of TC19-containing hypromellose gel significantly reduced numbers of viable methicillin-resistant Staphylococcus aureus (MRSA) and MDR Acinetobacter baumannii in a superficial wound infection in mice. Together, TC19 is an attractive candidate for further development as a novel agent against (MDR) bacterial skin wound infections.",
keywords = "Antibacterial activity, Thrombocidin-derived peptide, Wound infection",
author = "Martijn Riool and {de Breij}, Anna and Kwakman, {Paulus H. S.} and Elisabeth Schonkeren-Ravensbergen and {de Boer}, Leonie and Cordfunke, {Robert A.} and Nermina Malanovic and Drijfhout, {Jan W.} and Nibbering, {Peter H.} and Zaat, {Sebastian A. J.}",
year = "2020",
month = aug,
day = "1",
doi = "10.1016/j.bbamem.2020.183282",
language = "English",
volume = "1862",
journal = "BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES",
issn = "0005-2736",
publisher = "Elsevier",
number = "8",

}

RIS

TY - JOUR

T1 - Thrombocidin-1-derived antimicrobial peptide TC19 combats superficial multi-drug resistant bacterial wound infections

AU - Riool, Martijn

AU - de Breij, Anna

AU - Kwakman, Paulus H. S.

AU - Schonkeren-Ravensbergen, Elisabeth

AU - de Boer, Leonie

AU - Cordfunke, Robert A.

AU - Malanovic, Nermina

AU - Drijfhout, Jan W.

AU - Nibbering, Peter H.

AU - Zaat, Sebastian A. J.

PY - 2020/8/1

Y1 - 2020/8/1

N2 - Antimicrobial peptides are considered promising candidates for the development of novel antimicrobial agents to combat infections by multi-drug-resistant (MDR) bacteria. Here, we describe the identification and characterization of the synthetic peptide TC19, derived from the human thrombocidin-1-derived peptide L3. Biophysical experiments into the interaction between TC19 and mimics of human and bacterial plasma membranes demonstrated that the peptide is highly selective for bacterial membranes. In agreement, TC19 combined low cytotoxicity towards human fibroblasts with efficient and rapid killing in human plasma of MDR strains of several bacterial species of the ESKAPE panel. In addition, TC19 induced minor resistance in vitro, neutralized pro-inflammatory activity of bacterial cell envelope components while displaying slight chemotactic activity for human neutrophils. Importantly, topical application of TC19-containing hypromellose gel significantly reduced numbers of viable methicillin-resistant Staphylococcus aureus (MRSA) and MDR Acinetobacter baumannii in a superficial wound infection in mice. Together, TC19 is an attractive candidate for further development as a novel agent against (MDR) bacterial skin wound infections.

AB - Antimicrobial peptides are considered promising candidates for the development of novel antimicrobial agents to combat infections by multi-drug-resistant (MDR) bacteria. Here, we describe the identification and characterization of the synthetic peptide TC19, derived from the human thrombocidin-1-derived peptide L3. Biophysical experiments into the interaction between TC19 and mimics of human and bacterial plasma membranes demonstrated that the peptide is highly selective for bacterial membranes. In agreement, TC19 combined low cytotoxicity towards human fibroblasts with efficient and rapid killing in human plasma of MDR strains of several bacterial species of the ESKAPE panel. In addition, TC19 induced minor resistance in vitro, neutralized pro-inflammatory activity of bacterial cell envelope components while displaying slight chemotactic activity for human neutrophils. Importantly, topical application of TC19-containing hypromellose gel significantly reduced numbers of viable methicillin-resistant Staphylococcus aureus (MRSA) and MDR Acinetobacter baumannii in a superficial wound infection in mice. Together, TC19 is an attractive candidate for further development as a novel agent against (MDR) bacterial skin wound infections.

KW - Antibacterial activity

KW - Thrombocidin-derived peptide

KW - Wound infection

UR - http://www.scopus.com/inward/record.url?scp=85084416277&partnerID=8YFLogxK

U2 - 10.1016/j.bbamem.2020.183282

DO - 10.1016/j.bbamem.2020.183282

M3 - Article

C2 - 32376222

VL - 1862

JO - BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

JF - BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

SN - 0005-2736

IS - 8

M1 - 183282

ER -

ID: 11609013