Research output: Contribution to journal › Article › Academic › peer-review
The female mouse is resistant to mild vitamin B3 deficiency. / van der Stelt, Inge; Shi, Wenbiao; Bekkenkamp-Grovenstein, Melissa et al.
In: European journal of nutrition, Vol. 61, No. 1, 15.02.2022, p. 329-340.Research output: Contribution to journal › Article › Academic › peer-review
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TY - JOUR
T1 - The female mouse is resistant to mild vitamin B3 deficiency
AU - van der Stelt, Inge
AU - Shi, Wenbiao
AU - Bekkenkamp-Grovenstein, Melissa
AU - Zapata-Pérez, Rubén
AU - Houtkooper, Riekelt H.
AU - de Boer, Vincent C. J.
AU - Hegeman, Maria A.
AU - Keijer, Jaap
N1 - Funding Information: WS is supported by a Chinese Scholarship Council, Grant Number: #201303250054. RZP is supported by a postdoctoral grant from the European Union’s Horizon 2020 research and innovation programme. Marie Skłodowska-Curie Grant Agreement Number 840110. Publisher Copyright: © 2021, The Author(s). Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2022/2/15
Y1 - 2022/2/15
N2 - Purpose: Vitamin B3 provides nicotinamide adenine dinucleotide (NAD+), an essential coenzyme in oxidoreductase reactions. Severe vitamin B3 deficiency leads to the disease Pellagra, while mild vitamin B3 deficiency has been linked to age-related and metabolic diseases. Mild vitamin B3 deficiency is understudied, especially in females. Therefore, we examined how female mice responded to a diet that induced mild vitamin B3 deficiency in male mice. Methods: Female C57BL/6RccHsd mice were subjected for 18 weeks to a diet without vitamin B3 and low but sufficient tryptophan (0.115%) (0NR) and were compared to control female mice on the same diet with the reference dose of vitamin B3 (30NR, 30 mg nicotinamide riboside/ kg diet). Results: In the female mice, no differences between the two dietary groups were found in liver nicotinamide mononucleotide (NMN) levels, body composition, whole body energy and substrate metabolism measured by indirect calorimetry, or liver triacylglycerol metabolism. Expression of seven genes that previously were shown to respond to mild vitamin B3 deficiency in male white adipose tissue were not differentially expressed between the female dietary groups, neither was insulin sensitivity. Conclusion: We concluded that the female 0NR mice were not vitamin B3 deficient; the role of age, sex and health status is discussed. Demonstrated by clear differences between females and males, the latter showing mild deficiency under the same conditions, this study highlights the importance of studying both sexes.
AB - Purpose: Vitamin B3 provides nicotinamide adenine dinucleotide (NAD+), an essential coenzyme in oxidoreductase reactions. Severe vitamin B3 deficiency leads to the disease Pellagra, while mild vitamin B3 deficiency has been linked to age-related and metabolic diseases. Mild vitamin B3 deficiency is understudied, especially in females. Therefore, we examined how female mice responded to a diet that induced mild vitamin B3 deficiency in male mice. Methods: Female C57BL/6RccHsd mice were subjected for 18 weeks to a diet without vitamin B3 and low but sufficient tryptophan (0.115%) (0NR) and were compared to control female mice on the same diet with the reference dose of vitamin B3 (30NR, 30 mg nicotinamide riboside/ kg diet). Results: In the female mice, no differences between the two dietary groups were found in liver nicotinamide mononucleotide (NMN) levels, body composition, whole body energy and substrate metabolism measured by indirect calorimetry, or liver triacylglycerol metabolism. Expression of seven genes that previously were shown to respond to mild vitamin B3 deficiency in male white adipose tissue were not differentially expressed between the female dietary groups, neither was insulin sensitivity. Conclusion: We concluded that the female 0NR mice were not vitamin B3 deficient; the role of age, sex and health status is discussed. Demonstrated by clear differences between females and males, the latter showing mild deficiency under the same conditions, this study highlights the importance of studying both sexes.
KW - Insulin sensitivity
KW - Male–female differences
KW - Nicotinamide riboside
KW - Tryptophan
KW - Vitamin B deficiency
KW - White adipose tissue
UR - http://www.scopus.com/inward/record.url?scp=85111623212&partnerID=8YFLogxK
U2 - 10.1007/s00394-021-02651-8
DO - 10.1007/s00394-021-02651-8
M3 - Article
C2 - 34338868
VL - 61
SP - 329
EP - 340
JO - European journal of nutrition
JF - European journal of nutrition
SN - 1436-6207
IS - 1
ER -
ID: 19196386