The dietary intake of carrot‐derived rhamnogalacturonan‐I accelerates and augments the innate immune and anti‐viral interferon response to rhinovirus infection and reduces duration and severity of symptoms in humans in a randomized trial

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The dietary intake of carrot‐derived rhamnogalacturonan‐I accelerates and augments the innate immune and anti‐viral interferon response to rhinovirus infection and reduces duration and severity of symptoms in humans in a randomized trial. / Lutter, René ; Teitsma‐jansen, Annemarie; Floris, Esther et al.

In: Nutrients, Vol. 13, No. 12, 4395, 01.12.2021.

Research output: Contribution to journal › Article › Academic › peer-review

BibTeX

@article{af2709c7ad174ae4a6041bd61b776596,

title = "The dietary intake of carrot‐derived rhamnogalacturonan‐I accelerates and augments the innate immune and anti‐viral interferon response to rhinovirus infection and reduces duration and severity of symptoms in humans in a randomized trial",

abstract = "Acute respiratory infections are an important health concern. Traditionally, polysaccharide‐enriched extracts from plants, containing immunomodulatory rhamnogalacturonan‐I (RG‐1), were used prophylactically. We established the effects of dietary supplementation with carrot‐derived RG‐I (cRG‐I, 0–0.3–1.5 g/day) in 177 healthy individuals (18– 65 years) on symptoms following infection with rhinovirus strain 16 (RV16). Primary outcomes were changes in severity and duration of symptoms, and viral load in nasal lavage. Secondary outcomes were changes in innate immune and anti‐viral responses, reflected by CXCL10 and CXCL8 levels and cell differentials in nasal lavage. In a nested cohort, exploratory transcriptome analysis was conducted on nasal epithelium. Intake of cRG‐I was safe, well‐tolerated and accelerated local cellular and humoral innate immune responses induced by RV16 infection, with the strongest effects at 1.5 g/d. At 0.3 g/d, a faster interferon‐induced response, induction of the key anti‐viral gene EIF2AK2, faster viral clearance, and reduced symptom severity (−20%) and duration (−25%) were observed. Anti‐viral responses, viral clearance and symptom scores at 1.5 g/d were in between those of 0 and 0.3 g/d, suggesting a negative feedback loop preventing excessive interferon responses. Dietary intake of cRG‐I accelerated innate immune and antiviral responses, and reduced symptoms of an acute respiratory viral infection.",

keywords = "Anti‐viral response, Common cold, Healthy adults, Kinetics, Rhinovirus‐16, Transcriptome, Viral clearance",

author = "Ren{\'e} Lutter and Annemarie Teitsma‐jansen and Esther Floris and Saeeda Lone‐latif and Abilash Ravi and {Sabogal Pineros}, {Yanaika S.} and Tamara Dekker and Barbara Smids and Ridha Khurshid and Marcela Aparicio‐vergara and Rianne Ruijschop and Lara Ravanetti and Wim Calame and Alwine Kardinaal and Ruud Albers",

note = "Funding Information: This project was supported by Nutrileads and the European Union?s Horizon 2020 research and innovation program, grant agreements EFRO OP?OOST 2014?2020 CHALLENGE PROJ?00676, SME grant No 811592 and Eurostars E! 10574?NIMF. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = dec,

day = "1",

doi = "10.3390/nu13124395",

language = "English",

volume = "13",

journal = "Nutrients",

issn = "2072-6643",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "12",

}

RIS

TY - JOUR

T1 - The dietary intake of carrot‐derived rhamnogalacturonan‐I accelerates and augments the innate immune and anti‐viral interferon response to rhinovirus infection and reduces duration and severity of symptoms in humans in a randomized trial

AU - Lutter, René

AU - Teitsma‐jansen, Annemarie

AU - Floris, Esther

AU - Lone‐latif, Saeeda

AU - Ravi, Abilash

AU - Sabogal Pineros, Yanaika S.

AU - Dekker, Tamara

AU - Smids, Barbara

AU - Khurshid, Ridha

AU - Aparicio‐vergara, Marcela

AU - Ruijschop, Rianne

AU - Ravanetti, Lara

AU - Calame, Wim

AU - Kardinaal, Alwine

AU - Albers, Ruud

N1 - Funding Information: This project was supported by Nutrileads and the European Union?s Horizon 2020 research and innovation program, grant agreements EFRO OP?OOST 2014?2020 CHALLENGE PROJ?00676, SME grant No 811592 and Eurostars E! 10574?NIMF. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/12/1

Y1 - 2021/12/1

N2 - Acute respiratory infections are an important health concern. Traditionally, polysaccharide‐enriched extracts from plants, containing immunomodulatory rhamnogalacturonan‐I (RG‐1), were used prophylactically. We established the effects of dietary supplementation with carrot‐derived RG‐I (cRG‐I, 0–0.3–1.5 g/day) in 177 healthy individuals (18– 65 years) on symptoms following infection with rhinovirus strain 16 (RV16). Primary outcomes were changes in severity and duration of symptoms, and viral load in nasal lavage. Secondary outcomes were changes in innate immune and anti‐viral responses, reflected by CXCL10 and CXCL8 levels and cell differentials in nasal lavage. In a nested cohort, exploratory transcriptome analysis was conducted on nasal epithelium. Intake of cRG‐I was safe, well‐tolerated and accelerated local cellular and humoral innate immune responses induced by RV16 infection, with the strongest effects at 1.5 g/d. At 0.3 g/d, a faster interferon‐induced response, induction of the key anti‐viral gene EIF2AK2, faster viral clearance, and reduced symptom severity (−20%) and duration (−25%) were observed. Anti‐viral responses, viral clearance and symptom scores at 1.5 g/d were in between those of 0 and 0.3 g/d, suggesting a negative feedback loop preventing excessive interferon responses. Dietary intake of cRG‐I accelerated innate immune and antiviral responses, and reduced symptoms of an acute respiratory viral infection.

AB - Acute respiratory infections are an important health concern. Traditionally, polysaccharide‐enriched extracts from plants, containing immunomodulatory rhamnogalacturonan‐I (RG‐1), were used prophylactically. We established the effects of dietary supplementation with carrot‐derived RG‐I (cRG‐I, 0–0.3–1.5 g/day) in 177 healthy individuals (18– 65 years) on symptoms following infection with rhinovirus strain 16 (RV16). Primary outcomes were changes in severity and duration of symptoms, and viral load in nasal lavage. Secondary outcomes were changes in innate immune and anti‐viral responses, reflected by CXCL10 and CXCL8 levels and cell differentials in nasal lavage. In a nested cohort, exploratory transcriptome analysis was conducted on nasal epithelium. Intake of cRG‐I was safe, well‐tolerated and accelerated local cellular and humoral innate immune responses induced by RV16 infection, with the strongest effects at 1.5 g/d. At 0.3 g/d, a faster interferon‐induced response, induction of the key anti‐viral gene EIF2AK2, faster viral clearance, and reduced symptom severity (−20%) and duration (−25%) were observed. Anti‐viral responses, viral clearance and symptom scores at 1.5 g/d were in between those of 0 and 0.3 g/d, suggesting a negative feedback loop preventing excessive interferon responses. Dietary intake of cRG‐I accelerated innate immune and antiviral responses, and reduced symptoms of an acute respiratory viral infection.

KW - Anti‐viral response

KW - Common cold

KW - Healthy adults

KW - Kinetics

KW - Rhinovirus‐16

KW - Transcriptome

KW - Viral clearance

UR - http://www.scopus.com/inward/record.url?scp=85120670638&partnerID=8YFLogxK

U2 - 10.3390/nu13124395

DO - 10.3390/nu13124395

M3 - Article

C2 - 34959949

VL - 13

JO - Nutrients

JF - Nutrients

SN - 2072-6643

IS - 12

M1 - 4395

ER -

ID: 20729385

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