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The bivariate NRIP1/ZEB2 RNA marker permits non-invasive presymptomatic screening of pre-eclampsia. / Manders, Vera; Visser, Allerdien; Keijser, Remco; Min, Naomi; Poutsma, Ankie; Mulders, Joyce; van den Berkmortel, Tarah; Hortensius, Marjolein; Jongejan, Aldo; Pajkrt, Eva; Sistermans, Erik A; Sie, Daoud; Best, Myron G; Würdinger, Tom; de Boer, Marjon; Afink, Gijs; Oudejans, Cees.

In: Scientific reports, Vol. 10, No. 1, 14.12.2020, p. 21857.

Research output: Contribution to journalArticleAcademicpeer-review

Harvard

Manders, V, Visser, A, Keijser, R, Min, N, Poutsma, A, Mulders, J, van den Berkmortel, T, Hortensius, M, Jongejan, A, Pajkrt, E, Sistermans, EA, Sie, D, Best, MG, Würdinger, T, de Boer, M, Afink, G & Oudejans, C 2020, 'The bivariate NRIP1/ZEB2 RNA marker permits non-invasive presymptomatic screening of pre-eclampsia', Scientific reports, vol. 10, no. 1, pp. 21857. https://doi.org/10.1038/s41598-020-79008-4

APA

Manders, V., Visser, A., Keijser, R., Min, N., Poutsma, A., Mulders, J., van den Berkmortel, T., Hortensius, M., Jongejan, A., Pajkrt, E., Sistermans, E. A., Sie, D., Best, M. G., Würdinger, T., de Boer, M., Afink, G., & Oudejans, C. (2020). The bivariate NRIP1/ZEB2 RNA marker permits non-invasive presymptomatic screening of pre-eclampsia. Scientific reports, 10(1), 21857. https://doi.org/10.1038/s41598-020-79008-4

Vancouver

Author

Manders, Vera ; Visser, Allerdien ; Keijser, Remco ; Min, Naomi ; Poutsma, Ankie ; Mulders, Joyce ; van den Berkmortel, Tarah ; Hortensius, Marjolein ; Jongejan, Aldo ; Pajkrt, Eva ; Sistermans, Erik A ; Sie, Daoud ; Best, Myron G ; Würdinger, Tom ; de Boer, Marjon ; Afink, Gijs ; Oudejans, Cees. / The bivariate NRIP1/ZEB2 RNA marker permits non-invasive presymptomatic screening of pre-eclampsia. In: Scientific reports. 2020 ; Vol. 10, No. 1. pp. 21857.

BibTeX

@article{76c75a0791ee43ecb08e9af2d9856631,
title = "The bivariate NRIP1/ZEB2 RNA marker permits non-invasive presymptomatic screening of pre-eclampsia",
abstract = "Using genome-wide transcriptome analysis by RNA sequencing of first trimester plasma RNA, we tested whether the identification of pregnancies at risk of developing pre-eclampsia with or without preterm birth or growth restriction is possible between weeks 9-14, prior to the appearance of clinical symptoms. We implemented a metaheuristic approach in the self-learning SVM algorithm for differential gene expression analysis of normal pregnancies (n = 108), affected pregnancies (n = 34) and non-pregnant controls (n = 19). Presymptomatic candidate markers for affected pregnancies were validated by RT-qPCR in first trimester samples (n = 34) from an independent cohort. PRKG1 was significantly downregulated in a subset of pregnancies with birth weights below the 10thpercentile as shared symptom. The NRIP1/ZEB2 ratio was found to be upregulated in pregnancies with pre-eclampsia or trisomy 21. Complementary quantitative analysis of both the linear and circular forms of NRIP1 permitted discrimination between pre-eclampsia and trisomy 21. Pre-eclamptic pregnancies showed an increase in linear NRIP1 compared to circular NRIP1, while trisomy 21 pregnancies did not.",
author = "Vera Manders and Allerdien Visser and Remco Keijser and Naomi Min and Ankie Poutsma and Joyce Mulders and {van den Berkmortel}, Tarah and Marjolein Hortensius and Aldo Jongejan and Eva Pajkrt and Sistermans, {Erik A} and Daoud Sie and Best, {Myron G} and Tom W{\"u}rdinger and {de Boer}, Marjon and Gijs Afink and Cees Oudejans",
year = "2020",
month = dec,
day = "14",
doi = "10.1038/s41598-020-79008-4",
language = "English",
volume = "10",
pages = "21857",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - The bivariate NRIP1/ZEB2 RNA marker permits non-invasive presymptomatic screening of pre-eclampsia

AU - Manders, Vera

AU - Visser, Allerdien

AU - Keijser, Remco

AU - Min, Naomi

AU - Poutsma, Ankie

AU - Mulders, Joyce

AU - van den Berkmortel, Tarah

AU - Hortensius, Marjolein

AU - Jongejan, Aldo

AU - Pajkrt, Eva

AU - Sistermans, Erik A

AU - Sie, Daoud

AU - Best, Myron G

AU - Würdinger, Tom

AU - de Boer, Marjon

AU - Afink, Gijs

AU - Oudejans, Cees

PY - 2020/12/14

Y1 - 2020/12/14

N2 - Using genome-wide transcriptome analysis by RNA sequencing of first trimester plasma RNA, we tested whether the identification of pregnancies at risk of developing pre-eclampsia with or without preterm birth or growth restriction is possible between weeks 9-14, prior to the appearance of clinical symptoms. We implemented a metaheuristic approach in the self-learning SVM algorithm for differential gene expression analysis of normal pregnancies (n = 108), affected pregnancies (n = 34) and non-pregnant controls (n = 19). Presymptomatic candidate markers for affected pregnancies were validated by RT-qPCR in first trimester samples (n = 34) from an independent cohort. PRKG1 was significantly downregulated in a subset of pregnancies with birth weights below the 10thpercentile as shared symptom. The NRIP1/ZEB2 ratio was found to be upregulated in pregnancies with pre-eclampsia or trisomy 21. Complementary quantitative analysis of both the linear and circular forms of NRIP1 permitted discrimination between pre-eclampsia and trisomy 21. Pre-eclamptic pregnancies showed an increase in linear NRIP1 compared to circular NRIP1, while trisomy 21 pregnancies did not.

AB - Using genome-wide transcriptome analysis by RNA sequencing of first trimester plasma RNA, we tested whether the identification of pregnancies at risk of developing pre-eclampsia with or without preterm birth or growth restriction is possible between weeks 9-14, prior to the appearance of clinical symptoms. We implemented a metaheuristic approach in the self-learning SVM algorithm for differential gene expression analysis of normal pregnancies (n = 108), affected pregnancies (n = 34) and non-pregnant controls (n = 19). Presymptomatic candidate markers for affected pregnancies were validated by RT-qPCR in first trimester samples (n = 34) from an independent cohort. PRKG1 was significantly downregulated in a subset of pregnancies with birth weights below the 10thpercentile as shared symptom. The NRIP1/ZEB2 ratio was found to be upregulated in pregnancies with pre-eclampsia or trisomy 21. Complementary quantitative analysis of both the linear and circular forms of NRIP1 permitted discrimination between pre-eclampsia and trisomy 21. Pre-eclamptic pregnancies showed an increase in linear NRIP1 compared to circular NRIP1, while trisomy 21 pregnancies did not.

UR - http://www.scopus.com/inward/record.url?scp=85098476020&partnerID=8YFLogxK

U2 - 10.1038/s41598-020-79008-4

DO - 10.1038/s41598-020-79008-4

M3 - Article

C2 - 33318568

VL - 10

SP - 21857

JO - Scientific reports

JF - Scientific reports

SN - 2045-2322

IS - 1

ER -

ID: 15084885