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The Analysis of Platelet-Derived circRNA Repertoire as Potential Diagnostic Biomarker for Non-Small Cell Lung Cancer. / D'Ambrosi, Silvia; Visser, Allerdien; Antunes-Ferreira, Mafalda; Poutsma, Ankie; Giannoukakos, Stavros; Sol, Nik; Sabrkhany, Siamack; Bahce, Idris; Kuijpers, Marijke J E; Oude Egbrink, Mirjam G A; Griffioen, Arjan W; Best, Myron G; Koppers-Lalic, Danijela; Oudejans, Cees; Würdinger, Thomas.

In: Cancers, Vol. 13, No. 18, 4644, 16.09.2021.

Research output: Contribution to journalArticleAcademicpeer-review

Harvard

D'Ambrosi, S, Visser, A, Antunes-Ferreira, M, Poutsma, A, Giannoukakos, S, Sol, N, Sabrkhany, S, Bahce, I, Kuijpers, MJE, Oude Egbrink, MGA, Griffioen, AW, Best, MG, Koppers-Lalic, D, Oudejans, C & Würdinger, T 2021, 'The Analysis of Platelet-Derived circRNA Repertoire as Potential Diagnostic Biomarker for Non-Small Cell Lung Cancer', Cancers, vol. 13, no. 18, 4644. https://doi.org/10.3390/cancers13184644

APA

D'Ambrosi, S., Visser, A., Antunes-Ferreira, M., Poutsma, A., Giannoukakos, S., Sol, N., Sabrkhany, S., Bahce, I., Kuijpers, M. J. E., Oude Egbrink, M. G. A., Griffioen, A. W., Best, M. G., Koppers-Lalic, D., Oudejans, C., & Würdinger, T. (2021). The Analysis of Platelet-Derived circRNA Repertoire as Potential Diagnostic Biomarker for Non-Small Cell Lung Cancer. Cancers, 13(18), [4644]. https://doi.org/10.3390/cancers13184644

Vancouver

D'Ambrosi S, Visser A, Antunes-Ferreira M, Poutsma A, Giannoukakos S, Sol N et al. The Analysis of Platelet-Derived circRNA Repertoire as Potential Diagnostic Biomarker for Non-Small Cell Lung Cancer. Cancers. 2021 Sep 16;13(18). 4644. https://doi.org/10.3390/cancers13184644

Author

D'Ambrosi, Silvia ; Visser, Allerdien ; Antunes-Ferreira, Mafalda ; Poutsma, Ankie ; Giannoukakos, Stavros ; Sol, Nik ; Sabrkhany, Siamack ; Bahce, Idris ; Kuijpers, Marijke J E ; Oude Egbrink, Mirjam G A ; Griffioen, Arjan W ; Best, Myron G ; Koppers-Lalic, Danijela ; Oudejans, Cees ; Würdinger, Thomas. / The Analysis of Platelet-Derived circRNA Repertoire as Potential Diagnostic Biomarker for Non-Small Cell Lung Cancer. In: Cancers. 2021 ; Vol. 13, No. 18.

BibTeX

@article{3bf896b38199492bb4eafe30e83ed888,
title = "The Analysis of Platelet-Derived circRNA Repertoire as Potential Diagnostic Biomarker for Non-Small Cell Lung Cancer",
abstract = "Tumor-educated Platelets (TEPs) have emerged as rich biosources of cancer-related RNA profiles in liquid biopsies applicable for cancer detection. Although human blood platelets have been found to be enriched in circular RNA (circRNA), no studies have investigated the potential of circRNA as platelet-derived biomarkers for cancer. In this proof-of-concept study, we examine whether the circRNA signature of blood platelets can be used as a liquid biopsy biomarker for the detection of non-small cell lung cancer (NSCLC). We analyzed the total RNA, extracted from the platelet samples collected from NSCLC patients and asymptomatic individuals, using RNA sequencing (RNA-Seq). Identification and quantification of known and novel circRNAs were performed using the accurate CircRNA finder suite (ACFS), followed by the differential transcript expression analysis using a modified version of our thromboSeq software. Out of 4732 detected circRNAs, we identified 411 circRNAs that are significantly (p-value < 0.05) differentially expressed between asymptomatic individuals and NSCLC patients. Using the false discovery rate (FDR) of 0.05 as cutoff, we selected the nuclear receptor-interacting protein 1 (NRIP1) circRNA (circNRIP1) as a potential biomarker candidate for further validation by reverse transcription-quantitative PCR (RT-qPCR). This analysis was performed on an independent cohort of platelet samples. The RT-qPCR results confirmed the RNA-Seq data analysis, with significant downregulation of circNRIP1 in platelets derived from NSCLC patients. Our findings suggest that circRNAs found in blood platelets may hold diagnostic biomarkers potential for the detection of NSCLC using liquid biopsies.",
keywords = "Biomarkers, Liquid biopsy, Non-small cell lung cancer, Platelets, circular RNA",
author = "Silvia D'Ambrosi and Allerdien Visser and Mafalda Antunes-Ferreira and Ankie Poutsma and Stavros Giannoukakos and Nik Sol and Siamack Sabrkhany and Idris Bahce and Kuijpers, {Marijke J E} and {Oude Egbrink}, {Mirjam G A} and Griffioen, {Arjan W} and Best, {Myron G} and Danijela Koppers-Lalic and Cees Oudejans and Thomas W{\"u}rdinger",
note = "Funding Information: Funding: The work of authors S.D., M.A.-F., S.G., D.K.-L. and T.W. has received funding from the European Union{\textquoteright}s Horizon 2020 research and innovation program under the Marie Sk{\l}odowska-Curie Grant Agreement No. 765492. D.K.-L. received funding from the Cancer Center Amsterdam (CCA) Foundation (Grant #CCA2017-2-16). Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2021",
month = sep,
day = "16",
doi = "10.3390/cancers13184644",
language = "English",
volume = "13",
journal = "Cancers",
issn = "2072-6694",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "18",

}

RIS

TY - JOUR

T1 - The Analysis of Platelet-Derived circRNA Repertoire as Potential Diagnostic Biomarker for Non-Small Cell Lung Cancer

AU - D'Ambrosi, Silvia

AU - Visser, Allerdien

AU - Antunes-Ferreira, Mafalda

AU - Poutsma, Ankie

AU - Giannoukakos, Stavros

AU - Sol, Nik

AU - Sabrkhany, Siamack

AU - Bahce, Idris

AU - Kuijpers, Marijke J E

AU - Oude Egbrink, Mirjam G A

AU - Griffioen, Arjan W

AU - Best, Myron G

AU - Koppers-Lalic, Danijela

AU - Oudejans, Cees

AU - Würdinger, Thomas

N1 - Funding Information: Funding: The work of authors S.D., M.A.-F., S.G., D.K.-L. and T.W. has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie Grant Agreement No. 765492. D.K.-L. received funding from the Cancer Center Amsterdam (CCA) Foundation (Grant #CCA2017-2-16). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/9/16

Y1 - 2021/9/16

N2 - Tumor-educated Platelets (TEPs) have emerged as rich biosources of cancer-related RNA profiles in liquid biopsies applicable for cancer detection. Although human blood platelets have been found to be enriched in circular RNA (circRNA), no studies have investigated the potential of circRNA as platelet-derived biomarkers for cancer. In this proof-of-concept study, we examine whether the circRNA signature of blood platelets can be used as a liquid biopsy biomarker for the detection of non-small cell lung cancer (NSCLC). We analyzed the total RNA, extracted from the platelet samples collected from NSCLC patients and asymptomatic individuals, using RNA sequencing (RNA-Seq). Identification and quantification of known and novel circRNAs were performed using the accurate CircRNA finder suite (ACFS), followed by the differential transcript expression analysis using a modified version of our thromboSeq software. Out of 4732 detected circRNAs, we identified 411 circRNAs that are significantly (p-value < 0.05) differentially expressed between asymptomatic individuals and NSCLC patients. Using the false discovery rate (FDR) of 0.05 as cutoff, we selected the nuclear receptor-interacting protein 1 (NRIP1) circRNA (circNRIP1) as a potential biomarker candidate for further validation by reverse transcription-quantitative PCR (RT-qPCR). This analysis was performed on an independent cohort of platelet samples. The RT-qPCR results confirmed the RNA-Seq data analysis, with significant downregulation of circNRIP1 in platelets derived from NSCLC patients. Our findings suggest that circRNAs found in blood platelets may hold diagnostic biomarkers potential for the detection of NSCLC using liquid biopsies.

AB - Tumor-educated Platelets (TEPs) have emerged as rich biosources of cancer-related RNA profiles in liquid biopsies applicable for cancer detection. Although human blood platelets have been found to be enriched in circular RNA (circRNA), no studies have investigated the potential of circRNA as platelet-derived biomarkers for cancer. In this proof-of-concept study, we examine whether the circRNA signature of blood platelets can be used as a liquid biopsy biomarker for the detection of non-small cell lung cancer (NSCLC). We analyzed the total RNA, extracted from the platelet samples collected from NSCLC patients and asymptomatic individuals, using RNA sequencing (RNA-Seq). Identification and quantification of known and novel circRNAs were performed using the accurate CircRNA finder suite (ACFS), followed by the differential transcript expression analysis using a modified version of our thromboSeq software. Out of 4732 detected circRNAs, we identified 411 circRNAs that are significantly (p-value < 0.05) differentially expressed between asymptomatic individuals and NSCLC patients. Using the false discovery rate (FDR) of 0.05 as cutoff, we selected the nuclear receptor-interacting protein 1 (NRIP1) circRNA (circNRIP1) as a potential biomarker candidate for further validation by reverse transcription-quantitative PCR (RT-qPCR). This analysis was performed on an independent cohort of platelet samples. The RT-qPCR results confirmed the RNA-Seq data analysis, with significant downregulation of circNRIP1 in platelets derived from NSCLC patients. Our findings suggest that circRNAs found in blood platelets may hold diagnostic biomarkers potential for the detection of NSCLC using liquid biopsies.

KW - Biomarkers

KW - Liquid biopsy

KW - Non-small cell lung cancer

KW - Platelets

KW - circular RNA

UR - http://www.scopus.com/inward/record.url?scp=85114934829&partnerID=8YFLogxK

U2 - 10.3390/cancers13184644

DO - 10.3390/cancers13184644

M3 - Article

C2 - 34572871

VL - 13

JO - Cancers

JF - Cancers

SN - 2072-6694

IS - 18

M1 - 4644

ER -

ID: 20860648