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@article{f954c733ec084b4aa7617e6748163af5,
title = "Supernatant of stored platelets causes lung inflammation and coagulopathy in a novel in vivo transfusion model",
abstract = "Transfusion-related acute lung injury is suggested to be a {"}2-hit{"} event resulting from priming and activation of pulmonary neutrophils. Activation may result from infusion of lysophosphatidylcholines (LysoPCs), which accumulate during storage of blood products. In the present study, we developed a syngeneic in vivo transfusion model to test whether storage of platelet concentrates (PLTs) results in lung injury in healthy rats as well as in a {"}2-hit{"} model using lipopolysac-charide-pretreated rats. In addition, the effect of washing of platelets was studied. In healthy rats, transfusion of aged PLTs caused mild lung inflammation. In LPS-pretreated rats, transfusion of aged PLTs, but not fresh PLTs, augmented pulmonary and systemic coagulopathy. When PLTs components were transfused separately, supernatant of aged PLTs, but not washed aged platelets, induced pulmonary injury in the {"}2-hit{"} model. Supernatants of aged PLTs contained increased concentrations of LysoPCs compared with fresh PLTs, which enhanced neutrophil priming activity in vitro. We conclude that transfusion of aged PLTs induces lung inflammation in healthy rats. In a {"}2-hit{"} model, aged PLTs contribute to pulmonary and systemic coagulopathy, which may be mediated by LysoPCs, which accumulate in the supernatant of PLTs during storage. (Blood. 2010; 116(8): 1360-1368)",
author = "Vlaar, {Alexander P. J.} and Hofstra, {Jorrit J.} and Wim Kulik and {van Lenthe}, Henk and Rienk Nieuwland and Schultz, {Marcus J.} and Levi, {Marcel M.} and Roelofs, {Joris J. T. H.} and Tool, {Anton T. J.} and {de Korte}, Dirk and Juffermans, {Nicole P.}",
year = "2010",
doi = "10.1182/blood-2009-10-248732",
language = "English",
volume = "116",
pages = "1360--1368",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "8",

}

RIS

TY - JOUR

T1 - Supernatant of stored platelets causes lung inflammation and coagulopathy in a novel in vivo transfusion model

AU - Vlaar, Alexander P. J.

AU - Hofstra, Jorrit J.

AU - Kulik, Wim

AU - van Lenthe, Henk

AU - Nieuwland, Rienk

AU - Schultz, Marcus J.

AU - Levi, Marcel M.

AU - Roelofs, Joris J. T. H.

AU - Tool, Anton T. J.

AU - de Korte, Dirk

AU - Juffermans, Nicole P.

PY - 2010

Y1 - 2010

N2 - Transfusion-related acute lung injury is suggested to be a "2-hit" event resulting from priming and activation of pulmonary neutrophils. Activation may result from infusion of lysophosphatidylcholines (LysoPCs), which accumulate during storage of blood products. In the present study, we developed a syngeneic in vivo transfusion model to test whether storage of platelet concentrates (PLTs) results in lung injury in healthy rats as well as in a "2-hit" model using lipopolysac-charide-pretreated rats. In addition, the effect of washing of platelets was studied. In healthy rats, transfusion of aged PLTs caused mild lung inflammation. In LPS-pretreated rats, transfusion of aged PLTs, but not fresh PLTs, augmented pulmonary and systemic coagulopathy. When PLTs components were transfused separately, supernatant of aged PLTs, but not washed aged platelets, induced pulmonary injury in the "2-hit" model. Supernatants of aged PLTs contained increased concentrations of LysoPCs compared with fresh PLTs, which enhanced neutrophil priming activity in vitro. We conclude that transfusion of aged PLTs induces lung inflammation in healthy rats. In a "2-hit" model, aged PLTs contribute to pulmonary and systemic coagulopathy, which may be mediated by LysoPCs, which accumulate in the supernatant of PLTs during storage. (Blood. 2010; 116(8): 1360-1368)

AB - Transfusion-related acute lung injury is suggested to be a "2-hit" event resulting from priming and activation of pulmonary neutrophils. Activation may result from infusion of lysophosphatidylcholines (LysoPCs), which accumulate during storage of blood products. In the present study, we developed a syngeneic in vivo transfusion model to test whether storage of platelet concentrates (PLTs) results in lung injury in healthy rats as well as in a "2-hit" model using lipopolysac-charide-pretreated rats. In addition, the effect of washing of platelets was studied. In healthy rats, transfusion of aged PLTs caused mild lung inflammation. In LPS-pretreated rats, transfusion of aged PLTs, but not fresh PLTs, augmented pulmonary and systemic coagulopathy. When PLTs components were transfused separately, supernatant of aged PLTs, but not washed aged platelets, induced pulmonary injury in the "2-hit" model. Supernatants of aged PLTs contained increased concentrations of LysoPCs compared with fresh PLTs, which enhanced neutrophil priming activity in vitro. We conclude that transfusion of aged PLTs induces lung inflammation in healthy rats. In a "2-hit" model, aged PLTs contribute to pulmonary and systemic coagulopathy, which may be mediated by LysoPCs, which accumulate in the supernatant of PLTs during storage. (Blood. 2010; 116(8): 1360-1368)

U2 - 10.1182/blood-2009-10-248732

DO - 10.1182/blood-2009-10-248732

M3 - Article

C2 - 20479286

VL - 116

SP - 1360

EP - 1368

JO - Blood

JF - Blood

SN - 0006-4971

IS - 8

ER -

ID: 1054932