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Red blood cell transfusion results in adhesion of neutrophils in human endotoxemia and in critically ill patients with sepsis. / van Hezel, Maike E.; Boshuizen, Margit; Peters, Anna L. et al.

In: Transfusion, Vol. 60, No. 2, 01.02.2020, p. 294-302.

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@article{c2e8324d481447ffbeb5e4b1c5cb9e40,
title = "Red blood cell transfusion results in adhesion of neutrophils in human endotoxemia and in critically ill patients with sepsis",
abstract = "BACKGROUND: Red blood cell (RBC) transfusion is associated with adverse effects, which may involve activation of the host immune response. The effect of RBC transfusion on neutrophil Reactive Oxygen Species (ROS) production and adhesion ex vivo was investigated in endotoxemic volunteers and in critically ill patients that received a RBC transfusion. We hypothesized that RBC transfusion would cause neutrophil activation, the extent of which depends on the storage time and the inflammatory status of the recipient. STUDY DESIGN AND METHODS: Volunteers were injected with lipopolysaccharide (LPS) and transfused with either saline, fresh, or stored autologous RBCs. In addition, 47 critically ill patients with and without sepsis receiving either fresh (<8 days) or standard stored RBC (2-35 days) were included. Neutrophils from healthy volunteers were incubated with the plasma samples from the endotoxemic volunteers and from the critically ill patients, after which priming of neutrophil ROS production and adhesion were assessed. RESULTS: In the endotoxemia model, ex vivo neutrophil adhesion, but not ROS production, was increased after transfusion, which was not affected by RBC storage duration. In the critically ill, ex vivo neutrophil ROS production was already increased prior to transfusion and was not increased following transfusion. Neutrophil adhesion was increased following transfusion, which was more notable in the septic patients than in non-septic patients. Transfusion of fresh RBCs, but not standard issued RBCs, resulted in enhanced ROS production in neutrophils. CONCLUSION: RBC transfusion was associated with increased neutrophil adhesion in a model of human endotoxemia as well as in critically ill patients with sepsis.",
author = "{van Hezel}, {Maike E.} and Margit Boshuizen and Peters, {Anna L.} and M. Straat and Vlaar, {Alexander P.} and {Spoelstra - de Man}, {Angelique M. E.} and Tanck, {Michael W. T.} and Tool, {Anton T. J.} and Beuger, {Boukje M.} and Kuijpers, {Taco W.} and Juffermans, {Nicole P.} and {van Bruggen}, Robin",
year = "2020",
month = feb,
day = "1",
doi = "10.1111/trf.15613",
language = "English",
volume = "60",
pages = "294--302",
journal = "Transfusion",
issn = "0041-1132",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Red blood cell transfusion results in adhesion of neutrophils in human endotoxemia and in critically ill patients with sepsis

AU - van Hezel, Maike E.

AU - Boshuizen, Margit

AU - Peters, Anna L.

AU - Straat, M.

AU - Vlaar, Alexander P.

AU - Spoelstra - de Man, Angelique M. E.

AU - Tanck, Michael W. T.

AU - Tool, Anton T. J.

AU - Beuger, Boukje M.

AU - Kuijpers, Taco W.

AU - Juffermans, Nicole P.

AU - van Bruggen, Robin

PY - 2020/2/1

Y1 - 2020/2/1

N2 - BACKGROUND: Red blood cell (RBC) transfusion is associated with adverse effects, which may involve activation of the host immune response. The effect of RBC transfusion on neutrophil Reactive Oxygen Species (ROS) production and adhesion ex vivo was investigated in endotoxemic volunteers and in critically ill patients that received a RBC transfusion. We hypothesized that RBC transfusion would cause neutrophil activation, the extent of which depends on the storage time and the inflammatory status of the recipient. STUDY DESIGN AND METHODS: Volunteers were injected with lipopolysaccharide (LPS) and transfused with either saline, fresh, or stored autologous RBCs. In addition, 47 critically ill patients with and without sepsis receiving either fresh (<8 days) or standard stored RBC (2-35 days) were included. Neutrophils from healthy volunteers were incubated with the plasma samples from the endotoxemic volunteers and from the critically ill patients, after which priming of neutrophil ROS production and adhesion were assessed. RESULTS: In the endotoxemia model, ex vivo neutrophil adhesion, but not ROS production, was increased after transfusion, which was not affected by RBC storage duration. In the critically ill, ex vivo neutrophil ROS production was already increased prior to transfusion and was not increased following transfusion. Neutrophil adhesion was increased following transfusion, which was more notable in the septic patients than in non-septic patients. Transfusion of fresh RBCs, but not standard issued RBCs, resulted in enhanced ROS production in neutrophils. CONCLUSION: RBC transfusion was associated with increased neutrophil adhesion in a model of human endotoxemia as well as in critically ill patients with sepsis.

AB - BACKGROUND: Red blood cell (RBC) transfusion is associated with adverse effects, which may involve activation of the host immune response. The effect of RBC transfusion on neutrophil Reactive Oxygen Species (ROS) production and adhesion ex vivo was investigated in endotoxemic volunteers and in critically ill patients that received a RBC transfusion. We hypothesized that RBC transfusion would cause neutrophil activation, the extent of which depends on the storage time and the inflammatory status of the recipient. STUDY DESIGN AND METHODS: Volunteers were injected with lipopolysaccharide (LPS) and transfused with either saline, fresh, or stored autologous RBCs. In addition, 47 critically ill patients with and without sepsis receiving either fresh (<8 days) or standard stored RBC (2-35 days) were included. Neutrophils from healthy volunteers were incubated with the plasma samples from the endotoxemic volunteers and from the critically ill patients, after which priming of neutrophil ROS production and adhesion were assessed. RESULTS: In the endotoxemia model, ex vivo neutrophil adhesion, but not ROS production, was increased after transfusion, which was not affected by RBC storage duration. In the critically ill, ex vivo neutrophil ROS production was already increased prior to transfusion and was not increased following transfusion. Neutrophil adhesion was increased following transfusion, which was more notable in the septic patients than in non-septic patients. Transfusion of fresh RBCs, but not standard issued RBCs, resulted in enhanced ROS production in neutrophils. CONCLUSION: RBC transfusion was associated with increased neutrophil adhesion in a model of human endotoxemia as well as in critically ill patients with sepsis.

UR - http://www.scopus.com/inward/record.url?scp=85076086181&partnerID=8YFLogxK

U2 - 10.1111/trf.15613

DO - 10.1111/trf.15613

M3 - Article

C2 - 31804732

VL - 60

SP - 294

EP - 302

JO - Transfusion

JF - Transfusion

SN - 0041-1132

IS - 2

ER -

ID: 7773924