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Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure. / López-Labrador, F. Xavier; Brown, Julianne R.; Fischer, Nicole et al.

In: Journal of clinical virology, Vol. 134, 104691, 01.01.2021.

Research output: Contribution to journal › Article › Academic › peer-review

Harvard

López-Labrador, FX, Brown, JR, Fischer, N, Harvala, H, van Boheemen, S, Cinek, O, Sayiner, A, Madsen, TV, Auvinen, E, Kufner, V, Huber, M, Rodriguez, C, Jonges, M, Hönemann, M, Susi, P, Sousa, H, Klapper, PE, Pérez-Cataluňa, A, Hernandez, M, Molenkamp, R, der Hoek, LV, Schuurman, R, Couto, N, Leuzinger, K, Simmonds, P, Beer, M, Höper, D, Kamminga, S, Feltkamp, MCW, Rodríguez-Díaz, J, Keyaerts, E, Nielsen, XC, Puchhammer-Stöckl, E, Kroes, ACM, Buesa, J, Breuer, J, Claas, ECJ & de Vries, JJC 2021, 'Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure', Journal of clinical virology, vol. 134, 104691. https://doi.org/10.1016/j.jcv.2020.104691

APA

López-Labrador, F. X., Brown, J. R., Fischer, N., Harvala, H., van Boheemen, S., Cinek, O., Sayiner, A., Madsen, T. V., Auvinen, E., Kufner, V., Huber, M., Rodriguez, C., Jonges, M., Hönemann, M., Susi, P., Sousa, H., Klapper, P. E., Pérez-Cataluňa, A., Hernandez, M., ... de Vries, J. J. C. (2021). Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure. Journal of clinical virology, 134, [104691]. https://doi.org/10.1016/j.jcv.2020.104691

Vancouver

López-Labrador FX, Brown JR, Fischer N, Harvala H, van Boheemen S, Cinek O et al. Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure. Journal of clinical virology. 2021 Jan 1;134:104691. doi: 10.1016/j.jcv.2020.104691

Author

López-Labrador, F. Xavier ; Brown, Julianne R. ; Fischer, Nicole et al. / Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure. In: Journal of clinical virology. 2021 ; Vol. 134.

BibTeX

@article{95b641ef4a974b47827a211cc0226f1f,
title = "Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure",
abstract = "Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.",
keywords = "High-throughput sequencing, Next-generation sequencing, Recommendations, Viral metagenomics, Wet lab",
author = "L{\'o}pez-Labrador, {F. Xavier} and Brown, {Julianne R.} and Nicole Fischer and Heli Harvala and {van Boheemen}, Sander and Ondrej Cinek and Arzu Sayiner and Madsen, {Tina Vasehus} and Eeva Auvinen and Verena Kufner and Michael Huber and Christophe Rodriguez and Marcel Jonges and Mario H{\"o}nemann and Petri Susi and Hugo Sousa and Klapper, {Paul E.} and Alba P{\'e}rez-Catalu{\v n}a and Marta Hernandez and Richard Molenkamp and {der Hoek}, {Lia van} and Rob Schuurman and Natacha Couto and Karoline Leuzinger and Peter Simmonds and Martin Beer and Dirk H{\"o}per and Sergio Kamminga and Feltkamp, {Mariet C. W.} and Jes{\'u}s Rodr{\'i}guez-D{\'i}az and Els Keyaerts and Nielsen, {Xiaohui Chen} and Elisabeth Puchhammer-St{\"o}ckl and Kroes, {Aloys C. M.} and Javier Buesa and Judy Breuer and Claas, {Eric C. J.} and {de Vries}, {Jutte J. C.}",
note = "Publisher Copyright: {\textcopyright} 2020 The Authors",
year = "2021",
month = jan,
day = "1",
doi = "10.1016/j.jcv.2020.104691",
language = "English",
volume = "134",
journal = "Journal of clinical virology",
issn = "1386-6532",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Recommendations for the introduction of metagenomic high-throughput sequencing in clinical virology, part I: Wet lab procedure

AU - López-Labrador, F. Xavier

AU - Brown, Julianne R.

AU - Fischer, Nicole

AU - Harvala, Heli

AU - van Boheemen, Sander

AU - Cinek, Ondrej

AU - Sayiner, Arzu

AU - Madsen, Tina Vasehus

AU - Auvinen, Eeva

AU - Kufner, Verena

AU - Huber, Michael

AU - Rodriguez, Christophe

AU - Jonges, Marcel

AU - Hönemann, Mario

AU - Susi, Petri

AU - Sousa, Hugo

AU - Klapper, Paul E.

AU - Pérez-Cataluňa, Alba

AU - Hernandez, Marta

AU - Molenkamp, Richard

AU - der Hoek, Lia van

AU - Schuurman, Rob

AU - Couto, Natacha

AU - Leuzinger, Karoline

AU - Simmonds, Peter

AU - Beer, Martin

AU - Höper, Dirk

AU - Kamminga, Sergio

AU - Feltkamp, Mariet C. W.

AU - Rodríguez-Díaz, Jesús

AU - Keyaerts, Els

AU - Nielsen, Xiaohui Chen

AU - Puchhammer-Stöckl, Elisabeth

AU - Kroes, Aloys C. M.

AU - Buesa, Javier

AU - Breuer, Judy

AU - Claas, Eric C. J.

AU - de Vries, Jutte J. C.

N1 - Publisher Copyright: © 2020 The Authors

PY - 2021/1/1

Y1 - 2021/1/1

N2 - Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.

AB - Metagenomic high-throughput sequencing (mHTS) is a hypothesis-free, universal pathogen detection technique for determination of the DNA/RNA sequences in a variety of sample types and infectious syndromes. mHTS is still in its early stages of translating into clinical application. To support the development, implementation and standardization of mHTS procedures for virus diagnostics, the European Society for Clinical Virology (ESCV) Network on Next-Generation Sequencing (ENNGS) has been established. The aim of ENNGS is to bring together professionals involved in mHTS for viral diagnostics to share methodologies and experiences, and to develop application recommendations. This manuscript aims to provide practical recommendations for the wet lab procedures necessary for implementation of mHTS for virus diagnostics and to give recommendations for development and validation of laboratory methods, including mHTS quality assurance, control and quality assessment protocols.

KW - High-throughput sequencing

KW - Next-generation sequencing

KW - Recommendations

KW - Viral metagenomics

KW - Wet lab

UR - http://www.scopus.com/inward/record.url?scp=85097141892&partnerID=8YFLogxK

U2 - 10.1016/j.jcv.2020.104691

DO - 10.1016/j.jcv.2020.104691

M3 - Article

C2 - 33278791

VL - 134

JO - Journal of clinical virology

JF - Journal of clinical virology

SN - 1386-6532

M1 - 104691

ER -

ID: 14481866