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Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro. / Schoppmeyer, Rouven; van Buul, Jaap D.

In: STAR Protocols, Vol. 3, No. 3, 101509, 16.09.2022.

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@article{b9170d68d4de4077b60b9501fcce2649,
title = "Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro",
abstract = "This protocol presents an assay for transmigration analysis of human cytotoxic T cells (CTL) under physiological flow in vitro. We describe detailed analysis steps of human CTL behavior, from adhesion to diapedesis, using live cell imaging which cannot be achieved by in vivo imaging. The flow system is made of 2D plastic surfaces covered by an endothelial monolayer limiting the system but allows for quantitative analysis of CTL behavior with high modifiability. For complete details on the use and execution of this protocol, please refer to Schoppmeyer et al. (2022).",
keywords = "Cell Biology, Immunology, Microscopy",
author = "Rouven Schoppmeyer and {van Buul}, {Jaap D.}",
note = "Funding Information: This work was supported by ZonMW NWO Vici grant #91819632 (R.S. and J.D.v.B.). Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = sep,
day = "16",
doi = "10.1016/j.xpro.2022.101509",
language = "English",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "3",

}

RIS

TY - JOUR

T1 - Protocol to analyze transmigration of human cytotoxic T cells under physiological flow conditions in vitro

AU - Schoppmeyer, Rouven

AU - van Buul, Jaap D.

N1 - Funding Information: This work was supported by ZonMW NWO Vici grant #91819632 (R.S. and J.D.v.B.). Publisher Copyright: © 2022 The Author(s)

PY - 2022/9/16

Y1 - 2022/9/16

N2 - This protocol presents an assay for transmigration analysis of human cytotoxic T cells (CTL) under physiological flow in vitro. We describe detailed analysis steps of human CTL behavior, from adhesion to diapedesis, using live cell imaging which cannot be achieved by in vivo imaging. The flow system is made of 2D plastic surfaces covered by an endothelial monolayer limiting the system but allows for quantitative analysis of CTL behavior with high modifiability. For complete details on the use and execution of this protocol, please refer to Schoppmeyer et al. (2022).

AB - This protocol presents an assay for transmigration analysis of human cytotoxic T cells (CTL) under physiological flow in vitro. We describe detailed analysis steps of human CTL behavior, from adhesion to diapedesis, using live cell imaging which cannot be achieved by in vivo imaging. The flow system is made of 2D plastic surfaces covered by an endothelial monolayer limiting the system but allows for quantitative analysis of CTL behavior with high modifiability. For complete details on the use and execution of this protocol, please refer to Schoppmeyer et al. (2022).

KW - Cell Biology

KW - Immunology

KW - Microscopy

UR - http://www.scopus.com/inward/record.url?scp=85133007827&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2022.101509

DO - 10.1016/j.xpro.2022.101509

M3 - Article

C2 - 35776649

VL - 3

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 3

M1 - 101509

ER -

ID: 25225399