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Potential Role of Soluble TRAIL in Epithelial Injury in Children with Severe RSV Infection. / Bem, Reinout A.; Bos, Albert P.; Wösten-van Asperen, Roelie M.; Bruijn, Martijn; Lutter, Rene; Sprick, Martin R.; van Woensel, Job B. M.

In: American journal of respiratory cell and molecular biology, Vol. 42, No. 6, 2010, p. 697-705.

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@article{09bcb44002314ff9a35a0f4cb747c43e,
title = "Potential Role of Soluble TRAIL in Epithelial Injury in Children with Severe RSV Infection",
abstract = "Lower respiratory tract infection by respiratory syncytial virus (RSV) is a frequent cause of acute lung injury in young children and infants. Studies in adults and animals suggest that tumor necrosis factor receptor (TNFR) ligands may mediate lung injury by causing apoptosis of epithelial cells. The main goal of the present study was to determine whether the TNF-related apoptosis-inducing ligand (Apo2L/TRAIL) pathway may be implicated in epithelial injury during severe RSV infection in children. We report elevated levels of soluble (s)TRAIL released by leukocytes in bronchoalveolar lavage fluid (BALF) of patients with RSV-associated respiratory failure (n = 22) as compared with mechanically ventilated patients without pulmonary illness (n = 7). Primary bronchial epithelial cells of children without pulmonary disease obtained by nonbronchoscopic cytobrushing expressed both death receptors TRAIL-R1 and -R2, and were found to be susceptible for cell death by human recombinant sTRAIL in vitro. Furthermore, BALF from a patient with RSV induced cell death in these cells, which was partly attenuated by inhibiting TRAIL signaling. These data suggest that the TRAIL pro-apoptotic pathway may contribute to lung epithelial injury in severe RSV infection in children",
author = "Bem, {Reinout A.} and Bos, {Albert P.} and {W{\"o}sten-van Asperen}, {Roelie M.} and Martijn Bruijn and Rene Lutter and Sprick, {Martin R.} and {van Woensel}, {Job B. M.}",
year = "2010",
doi = "10.1165/rcmb.2009-0100OC",
language = "English",
volume = "42",
pages = "697--705",
journal = "American journal of respiratory cell and molecular biology",
issn = "1044-1549",
publisher = "American Thoracic Society",
number = "6",

}

RIS

TY - JOUR

T1 - Potential Role of Soluble TRAIL in Epithelial Injury in Children with Severe RSV Infection

AU - Bem, Reinout A.

AU - Bos, Albert P.

AU - Wösten-van Asperen, Roelie M.

AU - Bruijn, Martijn

AU - Lutter, Rene

AU - Sprick, Martin R.

AU - van Woensel, Job B. M.

PY - 2010

Y1 - 2010

N2 - Lower respiratory tract infection by respiratory syncytial virus (RSV) is a frequent cause of acute lung injury in young children and infants. Studies in adults and animals suggest that tumor necrosis factor receptor (TNFR) ligands may mediate lung injury by causing apoptosis of epithelial cells. The main goal of the present study was to determine whether the TNF-related apoptosis-inducing ligand (Apo2L/TRAIL) pathway may be implicated in epithelial injury during severe RSV infection in children. We report elevated levels of soluble (s)TRAIL released by leukocytes in bronchoalveolar lavage fluid (BALF) of patients with RSV-associated respiratory failure (n = 22) as compared with mechanically ventilated patients without pulmonary illness (n = 7). Primary bronchial epithelial cells of children without pulmonary disease obtained by nonbronchoscopic cytobrushing expressed both death receptors TRAIL-R1 and -R2, and were found to be susceptible for cell death by human recombinant sTRAIL in vitro. Furthermore, BALF from a patient with RSV induced cell death in these cells, which was partly attenuated by inhibiting TRAIL signaling. These data suggest that the TRAIL pro-apoptotic pathway may contribute to lung epithelial injury in severe RSV infection in children

AB - Lower respiratory tract infection by respiratory syncytial virus (RSV) is a frequent cause of acute lung injury in young children and infants. Studies in adults and animals suggest that tumor necrosis factor receptor (TNFR) ligands may mediate lung injury by causing apoptosis of epithelial cells. The main goal of the present study was to determine whether the TNF-related apoptosis-inducing ligand (Apo2L/TRAIL) pathway may be implicated in epithelial injury during severe RSV infection in children. We report elevated levels of soluble (s)TRAIL released by leukocytes in bronchoalveolar lavage fluid (BALF) of patients with RSV-associated respiratory failure (n = 22) as compared with mechanically ventilated patients without pulmonary illness (n = 7). Primary bronchial epithelial cells of children without pulmonary disease obtained by nonbronchoscopic cytobrushing expressed both death receptors TRAIL-R1 and -R2, and were found to be susceptible for cell death by human recombinant sTRAIL in vitro. Furthermore, BALF from a patient with RSV induced cell death in these cells, which was partly attenuated by inhibiting TRAIL signaling. These data suggest that the TRAIL pro-apoptotic pathway may contribute to lung epithelial injury in severe RSV infection in children

U2 - 10.1165/rcmb.2009-0100OC

DO - 10.1165/rcmb.2009-0100OC

M3 - Article

C2 - 19635930

VL - 42

SP - 697

EP - 705

JO - American journal of respiratory cell and molecular biology

JF - American journal of respiratory cell and molecular biology

SN - 1044-1549

IS - 6

ER -

ID: 927273