Standard

Harvard

APA

Vancouver

Author

BibTeX

@article{09ba2ecae0a7491088369798726bfb93,
title = "Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS",
abstract = "We describe here a user-friendly analysis protocol for semi-targeted polar metabolomics in human muscle biopsies using Zwitterionic Hydrophilic Interaction Liquid Chromatography and high-resolution full-scan mass spectrometry. Previously, this protocol has been used for Caenorhabditis elegans. Here we show that it can be successfully applied to human muscle biopsies with minor adjustments. Summarized instructions for other matrices are also provided. As peak integration in metabolomics can be challenging, we provide expected retention times and extensive peak descriptions to aid this process. For complete details on the use and execution of this protocol, please refer to Molenaars et al. (2021).",
keywords = "Health Sciences, Mass Spectrometry, Metabolism, Metabolomics",
author = "Schomakers, {Bauke V.} and Jill Hermans and Jaspers, {Yorrick R. J.} and Gajja Salomons and Vaz, {Fr{\'e}d{\'e}ric M.} and {van Weeghel}, Michel and Houtkooper, {Riekelt H.}",
note = "Funding Information: Work in the Houtkooper group is financially supported by a grant from the Velux Stiftung (no. 1063 ). The graphical abstract was made with BioRender. Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = jun,
day = "17",
doi = "10.1016/j.xpro.2022.101302",
language = "English",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - Polar metabolomics in human muscle biopsies using a liquid-liquid extraction and full-scan LC-MS

AU - Schomakers, Bauke V.

AU - Hermans, Jill

AU - Jaspers, Yorrick R. J.

AU - Salomons, Gajja

AU - Vaz, Frédéric M.

AU - van Weeghel, Michel

AU - Houtkooper, Riekelt H.

N1 - Funding Information: Work in the Houtkooper group is financially supported by a grant from the Velux Stiftung (no. 1063 ). The graphical abstract was made with BioRender. Publisher Copyright: © 2022 The Author(s)

PY - 2022/6/17

Y1 - 2022/6/17

N2 - We describe here a user-friendly analysis protocol for semi-targeted polar metabolomics in human muscle biopsies using Zwitterionic Hydrophilic Interaction Liquid Chromatography and high-resolution full-scan mass spectrometry. Previously, this protocol has been used for Caenorhabditis elegans. Here we show that it can be successfully applied to human muscle biopsies with minor adjustments. Summarized instructions for other matrices are also provided. As peak integration in metabolomics can be challenging, we provide expected retention times and extensive peak descriptions to aid this process. For complete details on the use and execution of this protocol, please refer to Molenaars et al. (2021).

AB - We describe here a user-friendly analysis protocol for semi-targeted polar metabolomics in human muscle biopsies using Zwitterionic Hydrophilic Interaction Liquid Chromatography and high-resolution full-scan mass spectrometry. Previously, this protocol has been used for Caenorhabditis elegans. Here we show that it can be successfully applied to human muscle biopsies with minor adjustments. Summarized instructions for other matrices are also provided. As peak integration in metabolomics can be challenging, we provide expected retention times and extensive peak descriptions to aid this process. For complete details on the use and execution of this protocol, please refer to Molenaars et al. (2021).

KW - Health Sciences

KW - Mass Spectrometry

KW - Metabolism

KW - Metabolomics

UR - http://www.scopus.com/inward/record.url?scp=85128412776&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2022.101302

DO - 10.1016/j.xpro.2022.101302

M3 - Article

C2 - 35479116

VL - 3

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 2

M1 - 101302

ER -

ID: 24141936