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Photochemical internalization enhances cytosolic release of antibiotic and increases its efficacy against staphylococcal infection. / Zhang, Xiaolin; de Boer, Leonie; Heiliegers, Laura et al.

In: Journal of controlled release, Vol. 283, 2018, p. 214-222.

Research output: Contribution to journal › Article › Academic › peer-review

Harvard

Zhang, X, de Boer, L, Heiliegers, L, Man-Bovenkerk, S, Selbo, PLK, Drijfhout, JW, Høgset, A & Zaat, SAJ 2018, 'Photochemical internalization enhances cytosolic release of antibiotic and increases its efficacy against staphylococcal infection', Journal of controlled release, vol. 283, pp. 214-222. https://doi.org/10.1016/j.jconrel.2018.06.004

APA

Zhang, X., de Boer, L., Heiliegers, L., Man-Bovenkerk, S., Selbo, P. L. K., Drijfhout, J. W., Høgset, A., & Zaat, S. A. J. (2018). Photochemical internalization enhances cytosolic release of antibiotic and increases its efficacy against staphylococcal infection. Journal of controlled release, 283, 214-222. https://doi.org/10.1016/j.jconrel.2018.06.004

Vancouver

Zhang X, de Boer L, Heiliegers L, Man-Bovenkerk S, Selbo PLK, Drijfhout JW et al. Photochemical internalization enhances cytosolic release of antibiotic and increases its efficacy against staphylococcal infection. Journal of controlled release. 2018;283:214-222. doi: 10.1016/j.jconrel.2018.06.004

Author

Zhang, Xiaolin ; de Boer, Leonie ; Heiliegers, Laura et al. / Photochemical internalization enhances cytosolic release of antibiotic and increases its efficacy against staphylococcal infection. In: Journal of controlled release. 2018 ; Vol. 283. pp. 214-222.

BibTeX

@article{8c650fd0943c474aa01e3cfc82b98640,
title = "Photochemical internalization enhances cytosolic release of antibiotic and increases its efficacy against staphylococcal infection",
abstract = "Bacterial pathogens such as Staphylococcus aureus and Staphylococcus epidermidis can survive in different types of cells including professional phagocytes, causing intracellular infections. Antibiotic treatment of intracellular infections is often unsuccessful due to the low efficacy of most antibiotics inside cells. Therefore, novel techniques which can improve intracellular activity of antibiotics are urgently needed. We aimed to use photochemical internalization (PCI) to enhance cytosolic release of antibiotics from endocytic vesicles after internalization. Our results show that PCI indeed caused cytosolic release of gentamicin and significantly increased its efficacy against S. epidermidis in vitro in mouse macrophages. Upon illumination for 15 min, the killing of intracellular S. epidermidis in RAW 264.7 cells by 10 or 30 μg/ml gentamicin was increased to 1 or 3 CFU log, respectively, owing to the use of PCI, whereas no killing by gentamicin only without PCI was observed. Moreover, survival of S. aureus-infected zebrafish embryos was significantly improved by treatment with PCI-gentamicin. PCI improved the therapeutic efficacy of gentamicin at a dose of 0.1 ng per embryo to a level similar to that of a dose of 0.4 ng per embryo, indicating that PCI can lower the antibiotic dose required for treating (intracellular) staphylococcal infection. Thus, the present study shows that PCI is a promising novel approach to enhance the intracellular efficacy of antibiotics via cytosolic release, allowing them to reach intracellular bacteria. This will expand their therapeutic window and will increase the numbers of antibiotics which can be used for treatment of intracellular infections.",
author = "Xiaolin Zhang and {de Boer}, Leonie and Laura Heiliegers and Sandra Man-Bovenkerk and Selbo, {P. l Kristian} and Drijfhout, {Jan W.} and Anders H{\o}gset and Zaat, {Sebastian A. J.}",
year = "2018",
doi = "10.1016/j.jconrel.2018.06.004",
language = "English",
volume = "283",
pages = "214--222",
journal = "Journal of controlled release",
issn = "0168-3659",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Photochemical internalization enhances cytosolic release of antibiotic and increases its efficacy against staphylococcal infection

AU - Zhang, Xiaolin

AU - de Boer, Leonie

AU - Heiliegers, Laura

AU - Man-Bovenkerk, Sandra

AU - Selbo, P. l Kristian

AU - Drijfhout, Jan W.

AU - Høgset, Anders

AU - Zaat, Sebastian A. J.

PY - 2018

Y1 - 2018

N2 - Bacterial pathogens such as Staphylococcus aureus and Staphylococcus epidermidis can survive in different types of cells including professional phagocytes, causing intracellular infections. Antibiotic treatment of intracellular infections is often unsuccessful due to the low efficacy of most antibiotics inside cells. Therefore, novel techniques which can improve intracellular activity of antibiotics are urgently needed. We aimed to use photochemical internalization (PCI) to enhance cytosolic release of antibiotics from endocytic vesicles after internalization. Our results show that PCI indeed caused cytosolic release of gentamicin and significantly increased its efficacy against S. epidermidis in vitro in mouse macrophages. Upon illumination for 15 min, the killing of intracellular S. epidermidis in RAW 264.7 cells by 10 or 30 μg/ml gentamicin was increased to 1 or 3 CFU log, respectively, owing to the use of PCI, whereas no killing by gentamicin only without PCI was observed. Moreover, survival of S. aureus-infected zebrafish embryos was significantly improved by treatment with PCI-gentamicin. PCI improved the therapeutic efficacy of gentamicin at a dose of 0.1 ng per embryo to a level similar to that of a dose of 0.4 ng per embryo, indicating that PCI can lower the antibiotic dose required for treating (intracellular) staphylococcal infection. Thus, the present study shows that PCI is a promising novel approach to enhance the intracellular efficacy of antibiotics via cytosolic release, allowing them to reach intracellular bacteria. This will expand their therapeutic window and will increase the numbers of antibiotics which can be used for treatment of intracellular infections.

AB - Bacterial pathogens such as Staphylococcus aureus and Staphylococcus epidermidis can survive in different types of cells including professional phagocytes, causing intracellular infections. Antibiotic treatment of intracellular infections is often unsuccessful due to the low efficacy of most antibiotics inside cells. Therefore, novel techniques which can improve intracellular activity of antibiotics are urgently needed. We aimed to use photochemical internalization (PCI) to enhance cytosolic release of antibiotics from endocytic vesicles after internalization. Our results show that PCI indeed caused cytosolic release of gentamicin and significantly increased its efficacy against S. epidermidis in vitro in mouse macrophages. Upon illumination for 15 min, the killing of intracellular S. epidermidis in RAW 264.7 cells by 10 or 30 μg/ml gentamicin was increased to 1 or 3 CFU log, respectively, owing to the use of PCI, whereas no killing by gentamicin only without PCI was observed. Moreover, survival of S. aureus-infected zebrafish embryos was significantly improved by treatment with PCI-gentamicin. PCI improved the therapeutic efficacy of gentamicin at a dose of 0.1 ng per embryo to a level similar to that of a dose of 0.4 ng per embryo, indicating that PCI can lower the antibiotic dose required for treating (intracellular) staphylococcal infection. Thus, the present study shows that PCI is a promising novel approach to enhance the intracellular efficacy of antibiotics via cytosolic release, allowing them to reach intracellular bacteria. This will expand their therapeutic window and will increase the numbers of antibiotics which can be used for treatment of intracellular infections.

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85048573012&origin=inward

UR - https://www.ncbi.nlm.nih.gov/pubmed/29883696

U2 - 10.1016/j.jconrel.2018.06.004

DO - 10.1016/j.jconrel.2018.06.004

M3 - Article

C2 - 29883696

VL - 283

SP - 214

EP - 222

JO - Journal of controlled release

JF - Journal of controlled release

SN - 0168-3659

ER -

ID: 5528007