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Photochemical Internalization as a New Strategy to Enhance Efficacy of Antimicrobial Agents Against Intracellular Infections. / Zhang, Xiaolin; de Boer, Leonie; Zaat, Sebastian A. J.

Methods in Molecular Biology. Vol. 2451 Humana Press Inc., 2022. p. 671-689 (Methods in Molecular Biology; Vol. 2451).

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Harvard

Zhang, X, de Boer, L & Zaat, SAJ 2022, Photochemical Internalization as a New Strategy to Enhance Efficacy of Antimicrobial Agents Against Intracellular Infections. in Methods in Molecular Biology. vol. 2451, Methods in Molecular Biology, vol. 2451, Humana Press Inc., pp. 671-689. https://doi.org/10.1007/978-1-0716-2099-1_35

APA

Zhang, X., de Boer, L., & Zaat, S. A. J. (2022). Photochemical Internalization as a New Strategy to Enhance Efficacy of Antimicrobial Agents Against Intracellular Infections. In Methods in Molecular Biology (Vol. 2451, pp. 671-689). (Methods in Molecular Biology; Vol. 2451). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-2099-1_35

Vancouver

Zhang X, de Boer L, Zaat SAJ. Photochemical Internalization as a New Strategy to Enhance Efficacy of Antimicrobial Agents Against Intracellular Infections. In Methods in Molecular Biology. Vol. 2451. Humana Press Inc. 2022. p. 671-689. (Methods in Molecular Biology). doi: 10.1007/978-1-0716-2099-1_35

Author

Zhang, Xiaolin ; de Boer, Leonie ; Zaat, Sebastian A. J. / Photochemical Internalization as a New Strategy to Enhance Efficacy of Antimicrobial Agents Against Intracellular Infections. Methods in Molecular Biology. Vol. 2451 Humana Press Inc., 2022. pp. 671-689 (Methods in Molecular Biology).

BibTeX

@inbook{b3d1916c55e546249f2d5c9d362878e0,
title = "Photochemical Internalization as a New Strategy to Enhance Efficacy of Antimicrobial Agents Against Intracellular Infections",
abstract = "Pathogens such as Staphylococcus aureus are able to survive in many types of host cells including phagocytes such as neutrophils and macrophages, thereby resulting in intracellular infections. Treatment of intracellular infections by conventional antimicrobials (e.g., antibiotics) is often ineffective due to low intracellular efficacy of the drugs. Thus, novel techniques which can enhance the activity of antimicrobials within cells are highly demanded. Our recent studies have shown that photochemical internalization (PCI) is a promising approach for improving the efficacy of antibiotics such as gentamicin against intracellular staphylococcal infection. In this chapter, we describe the protocols aiming to study the potential of PCI-antibiotic treatment for intracellular infections in vitro and in vivo using a RAW 264.7 cell infection model and a zebrafish embryo infection model. Proof of concept of this approach is demonstrated. The protocols are expected to prompt further development of PCI-antimicrobial based novel therapies for clinically challenging infectious diseases associated with intracellular survival of pathogens.",
keywords = "Antibiotics, Cytosolic release, Intracellular infections, Mouse macrophage, Photochemical internalization (PCI), Staphylococci, Zebrafish embryo",
author = "Xiaolin Zhang and {de Boer}, Leonie and Zaat, {Sebastian A. J.}",
note = "Publisher Copyright: {\textcopyright} 2022, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2099-1_35",
language = "English",
volume = "2451",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "671--689",
booktitle = "Methods in Molecular Biology",

}

RIS

TY - CHAP

T1 - Photochemical Internalization as a New Strategy to Enhance Efficacy of Antimicrobial Agents Against Intracellular Infections

AU - Zhang, Xiaolin

AU - de Boer, Leonie

AU - Zaat, Sebastian A. J.

N1 - Publisher Copyright: © 2022, Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - Pathogens such as Staphylococcus aureus are able to survive in many types of host cells including phagocytes such as neutrophils and macrophages, thereby resulting in intracellular infections. Treatment of intracellular infections by conventional antimicrobials (e.g., antibiotics) is often ineffective due to low intracellular efficacy of the drugs. Thus, novel techniques which can enhance the activity of antimicrobials within cells are highly demanded. Our recent studies have shown that photochemical internalization (PCI) is a promising approach for improving the efficacy of antibiotics such as gentamicin against intracellular staphylococcal infection. In this chapter, we describe the protocols aiming to study the potential of PCI-antibiotic treatment for intracellular infections in vitro and in vivo using a RAW 264.7 cell infection model and a zebrafish embryo infection model. Proof of concept of this approach is demonstrated. The protocols are expected to prompt further development of PCI-antimicrobial based novel therapies for clinically challenging infectious diseases associated with intracellular survival of pathogens.

AB - Pathogens such as Staphylococcus aureus are able to survive in many types of host cells including phagocytes such as neutrophils and macrophages, thereby resulting in intracellular infections. Treatment of intracellular infections by conventional antimicrobials (e.g., antibiotics) is often ineffective due to low intracellular efficacy of the drugs. Thus, novel techniques which can enhance the activity of antimicrobials within cells are highly demanded. Our recent studies have shown that photochemical internalization (PCI) is a promising approach for improving the efficacy of antibiotics such as gentamicin against intracellular staphylococcal infection. In this chapter, we describe the protocols aiming to study the potential of PCI-antibiotic treatment for intracellular infections in vitro and in vivo using a RAW 264.7 cell infection model and a zebrafish embryo infection model. Proof of concept of this approach is demonstrated. The protocols are expected to prompt further development of PCI-antimicrobial based novel therapies for clinically challenging infectious diseases associated with intracellular survival of pathogens.

KW - Antibiotics

KW - Cytosolic release

KW - Intracellular infections

KW - Mouse macrophage

KW - Photochemical internalization (PCI)

KW - Staphylococci

KW - Zebrafish embryo

UR - http://www.scopus.com/inward/record.url?scp=85129572283&partnerID=8YFLogxK

U2 - 10.1007/978-1-0716-2099-1_35

DO - 10.1007/978-1-0716-2099-1_35

M3 - Chapter

C2 - 35505040

VL - 2451

T3 - Methods in Molecular Biology

SP - 671

EP - 689

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

ID: 23555055