Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review
Photochemical Internalization as a New Strategy to Enhance Efficacy of Antimicrobial Agents Against Intracellular Infections. / Zhang, Xiaolin; de Boer, Leonie; Zaat, Sebastian A. J.
Methods in Molecular Biology. Vol. 2451 Humana Press Inc., 2022. p. 671-689 (Methods in Molecular Biology; Vol. 2451).Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review
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TY - CHAP
T1 - Photochemical Internalization as a New Strategy to Enhance Efficacy of Antimicrobial Agents Against Intracellular Infections
AU - Zhang, Xiaolin
AU - de Boer, Leonie
AU - Zaat, Sebastian A. J.
N1 - Publisher Copyright: © 2022, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022
Y1 - 2022
N2 - Pathogens such as Staphylococcus aureus are able to survive in many types of host cells including phagocytes such as neutrophils and macrophages, thereby resulting in intracellular infections. Treatment of intracellular infections by conventional antimicrobials (e.g., antibiotics) is often ineffective due to low intracellular efficacy of the drugs. Thus, novel techniques which can enhance the activity of antimicrobials within cells are highly demanded. Our recent studies have shown that photochemical internalization (PCI) is a promising approach for improving the efficacy of antibiotics such as gentamicin against intracellular staphylococcal infection. In this chapter, we describe the protocols aiming to study the potential of PCI-antibiotic treatment for intracellular infections in vitro and in vivo using a RAW 264.7 cell infection model and a zebrafish embryo infection model. Proof of concept of this approach is demonstrated. The protocols are expected to prompt further development of PCI-antimicrobial based novel therapies for clinically challenging infectious diseases associated with intracellular survival of pathogens.
AB - Pathogens such as Staphylococcus aureus are able to survive in many types of host cells including phagocytes such as neutrophils and macrophages, thereby resulting in intracellular infections. Treatment of intracellular infections by conventional antimicrobials (e.g., antibiotics) is often ineffective due to low intracellular efficacy of the drugs. Thus, novel techniques which can enhance the activity of antimicrobials within cells are highly demanded. Our recent studies have shown that photochemical internalization (PCI) is a promising approach for improving the efficacy of antibiotics such as gentamicin against intracellular staphylococcal infection. In this chapter, we describe the protocols aiming to study the potential of PCI-antibiotic treatment for intracellular infections in vitro and in vivo using a RAW 264.7 cell infection model and a zebrafish embryo infection model. Proof of concept of this approach is demonstrated. The protocols are expected to prompt further development of PCI-antimicrobial based novel therapies for clinically challenging infectious diseases associated with intracellular survival of pathogens.
KW - Antibiotics
KW - Cytosolic release
KW - Intracellular infections
KW - Mouse macrophage
KW - Photochemical internalization (PCI)
KW - Staphylococci
KW - Zebrafish embryo
UR - http://www.scopus.com/inward/record.url?scp=85129572283&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-2099-1_35
DO - 10.1007/978-1-0716-2099-1_35
M3 - Chapter
C2 - 35505040
VL - 2451
T3 - Methods in Molecular Biology
SP - 671
EP - 689
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -
ID: 23555055