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Peroxisomal ATP Uptake Is Provided by Two Adenine Nucleotide Transporters and the ABCD Transporters. / van Roermund, Carlo W. T.; IJlst, Lodewijk; Linka, Nicole et al.

In: Frontiers in cell and developmental biology, Vol. 9, 788921, 19.01.2022.

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@article{70f25deb4ebe44fc91d771d110b41929,
title = "Peroxisomal ATP Uptake Is Provided by Two Adenine Nucleotide Transporters and the ABCD Transporters",
abstract = "Peroxisomes are essential organelles involved in various metabolic processes, including fatty acid β-oxidation. Their metabolic functions require a controlled exchange of metabolites and co-factors, including ATP, across the peroxisomal membrane. We investigated which proteins are involved in the peroxisomal uptake of ATP in the yeast Saccharomyces cerevisiae. Using wild-type and targeted deletion strains, we measured ATP-dependent peroxisomal octanoate β-oxidation, intra-peroxisomal ATP levels employing peroxisome-targeted ATP-sensing reporter proteins, and ATP uptake in proteoliposomes prepared from purified peroxisomes. We show that intra-peroxisomal ATP levels are maintained by different peroxisomal membrane proteins each with different modes of action: 1) the previously reported Ant1p protein, which catalyzes the exchange of ATP for AMP or ADP, 2) the ABC transporter protein complex Pxa1p/Pxa2p, which mediates both uni-directional acyl-CoA and ATP uptake, and 3) the mitochondrial Aac2p protein, which catalyzes ATP/ADP exchange and has a dual localization in both mitochondria and peroxisomes. Our results provide compelling evidence for a complementary system for the uptake of ATP in peroxisomes.",
keywords = "ABCD, ATP uptake, SLC25A, beta oxidation, peroxisome",
author = "{van Roermund}, {Carlo W. T.} and Lodewijk IJlst and Nicole Linka and Wanders, {Ronald J. A.} and Waterham, {Hans R.}",
note = "Funding Information: This work was in part funded by the German Research Foundation (DFG) via grants LI 1781/1-3 (to NL). Publisher Copyright: Copyright {\textcopyright} 2022 van Roermund, IJlst, Linka, Wanders and Waterham.",
year = "2022",
month = jan,
day = "19",
doi = "10.3389/fcell.2021.788921",
language = "English",
volume = "9",
journal = "Frontiers in cell and developmental biology",
issn = "2296-634X",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Peroxisomal ATP Uptake Is Provided by Two Adenine Nucleotide Transporters and the ABCD Transporters

AU - van Roermund, Carlo W. T.

AU - IJlst, Lodewijk

AU - Linka, Nicole

AU - Wanders, Ronald J. A.

AU - Waterham, Hans R.

N1 - Funding Information: This work was in part funded by the German Research Foundation (DFG) via grants LI 1781/1-3 (to NL). Publisher Copyright: Copyright © 2022 van Roermund, IJlst, Linka, Wanders and Waterham.

PY - 2022/1/19

Y1 - 2022/1/19

N2 - Peroxisomes are essential organelles involved in various metabolic processes, including fatty acid β-oxidation. Their metabolic functions require a controlled exchange of metabolites and co-factors, including ATP, across the peroxisomal membrane. We investigated which proteins are involved in the peroxisomal uptake of ATP in the yeast Saccharomyces cerevisiae. Using wild-type and targeted deletion strains, we measured ATP-dependent peroxisomal octanoate β-oxidation, intra-peroxisomal ATP levels employing peroxisome-targeted ATP-sensing reporter proteins, and ATP uptake in proteoliposomes prepared from purified peroxisomes. We show that intra-peroxisomal ATP levels are maintained by different peroxisomal membrane proteins each with different modes of action: 1) the previously reported Ant1p protein, which catalyzes the exchange of ATP for AMP or ADP, 2) the ABC transporter protein complex Pxa1p/Pxa2p, which mediates both uni-directional acyl-CoA and ATP uptake, and 3) the mitochondrial Aac2p protein, which catalyzes ATP/ADP exchange and has a dual localization in both mitochondria and peroxisomes. Our results provide compelling evidence for a complementary system for the uptake of ATP in peroxisomes.

AB - Peroxisomes are essential organelles involved in various metabolic processes, including fatty acid β-oxidation. Their metabolic functions require a controlled exchange of metabolites and co-factors, including ATP, across the peroxisomal membrane. We investigated which proteins are involved in the peroxisomal uptake of ATP in the yeast Saccharomyces cerevisiae. Using wild-type and targeted deletion strains, we measured ATP-dependent peroxisomal octanoate β-oxidation, intra-peroxisomal ATP levels employing peroxisome-targeted ATP-sensing reporter proteins, and ATP uptake in proteoliposomes prepared from purified peroxisomes. We show that intra-peroxisomal ATP levels are maintained by different peroxisomal membrane proteins each with different modes of action: 1) the previously reported Ant1p protein, which catalyzes the exchange of ATP for AMP or ADP, 2) the ABC transporter protein complex Pxa1p/Pxa2p, which mediates both uni-directional acyl-CoA and ATP uptake, and 3) the mitochondrial Aac2p protein, which catalyzes ATP/ADP exchange and has a dual localization in both mitochondria and peroxisomes. Our results provide compelling evidence for a complementary system for the uptake of ATP in peroxisomes.

KW - ABCD

KW - ATP uptake

KW - SLC25A

KW - beta oxidation

KW - peroxisome

UR - http://www.scopus.com/inward/record.url?scp=85124006507&partnerID=8YFLogxK

U2 - 10.3389/fcell.2021.788921

DO - 10.3389/fcell.2021.788921

M3 - Article

C2 - 35127709

VL - 9

JO - Frontiers in cell and developmental biology

JF - Frontiers in cell and developmental biology

SN - 2296-634X

M1 - 788921

ER -

ID: 21647681