Research output: Contribution to journal › Article › Academic › peer-review
Opportunities and short-comings of the axolotl salamander heart as a model system of human single ventricle and excessive trabeculation. / Meyer, Sophie; Lauridsen, Henrik; Pedersen, Kathrine et al.
In: Scientific reports, Vol. 12, No. 1, 20491, 01.12.2022, p. 20491.Research output: Contribution to journal › Article › Academic › peer-review
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TY - JOUR
T1 - Opportunities and short-comings of the axolotl salamander heart as a model system of human single ventricle and excessive trabeculation
AU - Meyer, Sophie
AU - Lauridsen, Henrik
AU - Pedersen, Kathrine
AU - Andersson, Sofie Amalie
AU - van Ooij, Pim
AU - Willems, Tineke
AU - Berger, Rolf M. F.
AU - Ebels, Tjark
AU - Jensen, Bjarke
N1 - Publisher Copyright: © 2022, The Author(s).
PY - 2022/12/1
Y1 - 2022/12/1
N2 - Few experimental model systems are available for the rare congenital heart diseases of double inlet left ventricle (DILV), a subgroup of univentricular hearts, and excessive trabeculation (ET), or noncompaction. Here, we explore the heart of the axolotl salamander (Ambystoma mexicanum, Shaw 1789) as model system of these diseases. Using micro-echocardiography, we assessed the form and function of the heart of the axolotl, an amphibian, and compared this to human DILV (n = 3). The main finding was that both in the axolotl and DILV, blood flows of disparate oxygen saturation can stay separated in a single ventricle. In the axolotl there is a solitary ventricular inlet and outlet, whereas in DILV there are two separate inlets and outlets. Axolotls had a lower resting heart rate compared to DILV (22 vs. 72 beats per minute), lower ejection fraction (47 vs. 58%), and their oxygen consumption at rest was higher than peak oxygen consumption in DILV (30 vs. 17 ml min−1 kg−1). Concerning the ventricular myocardial organization, histology showed trabeculations in ET (n = 5) are much closer to the normal human setting than to the axolotl setting. We conclude that the axolotl heart resembles some aspects of DILV and ET albeit substantial species differences exist.
AB - Few experimental model systems are available for the rare congenital heart diseases of double inlet left ventricle (DILV), a subgroup of univentricular hearts, and excessive trabeculation (ET), or noncompaction. Here, we explore the heart of the axolotl salamander (Ambystoma mexicanum, Shaw 1789) as model system of these diseases. Using micro-echocardiography, we assessed the form and function of the heart of the axolotl, an amphibian, and compared this to human DILV (n = 3). The main finding was that both in the axolotl and DILV, blood flows of disparate oxygen saturation can stay separated in a single ventricle. In the axolotl there is a solitary ventricular inlet and outlet, whereas in DILV there are two separate inlets and outlets. Axolotls had a lower resting heart rate compared to DILV (22 vs. 72 beats per minute), lower ejection fraction (47 vs. 58%), and their oxygen consumption at rest was higher than peak oxygen consumption in DILV (30 vs. 17 ml min−1 kg−1). Concerning the ventricular myocardial organization, histology showed trabeculations in ET (n = 5) are much closer to the normal human setting than to the axolotl setting. We conclude that the axolotl heart resembles some aspects of DILV and ET albeit substantial species differences exist.
KW - Humans
KW - Animals
KW - Ambystoma mexicanum
KW - Univentricular Heart
KW - Urodela
KW - Heart
KW - Cardiovascular Abnormalities
UR - http://www.scopus.com/inward/record.url?scp=85142829425&partnerID=8YFLogxK
U2 - 10.1038/s41598-022-24442-9
DO - 10.1038/s41598-022-24442-9
M3 - Article
C2 - 36443330
VL - 12
SP - 20491
JO - Scientific reports
JF - Scientific reports
SN - 2045-2322
IS - 1
M1 - 20491
ER -
ID: 28413181