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Neutrophil Extracellular Traps Do Not Induce Injury and Inflammation in Well-Differentiated RSV-Infected Airway Epithelium. / Linssen, Rosalie S. N.; Sridhar, Adithya; Moreni, Giulia et al.

In: Cells, Vol. 11, No. 5, 785, 01.03.2022.

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@article{16a17dd127c24b48b2b1b7b38f8b2fad,
title = "Neutrophil Extracellular Traps Do Not Induce Injury and Inflammation in Well-Differentiated RSV-Infected Airway Epithelium",
abstract = "Respiratory syncytial virus (RSV) lower respiratory tract infection (LRTI) causes a major burden of disease. The host response in RSV-LRTI is characterized by airway epithelial injury, inflammation and neutrophil influx, with the formation of neutrophil extracellular traps (NETs). However, the precise role of NETs in the pathophysiology of RSV-LRTI remains to be elucidated. Here, we used well-differentiated human airway epithelial cultures (HAE) of a pediatric and adult donor to study whether NETs cause airway epithelial injury and inflammation in the setting of RSV infection. The exposure of uninfected and RSV-infected HAE cultures to NETs, as produced by stimulation of neutrophils by a low dose of phorbol 12-myristate 13-acetate (PMA), did not induce or aggravate cell injury or inflammation. RSV infection of HAE cultures caused release of pro-inflammatory cytokines such as IL-6 and RANTES in both adult and pediatric cultures, but the differential gene expression for regulated cell death differed between culture donors. In this in vitro airway epithelial model, NETs in the setting of RSV infection did not cause or aggravate epithelial injury or inflammation.",
keywords = "Cell culture, Human airway epithelial, Infection, Neutrophil extracellular traps, Neutrophils, RSV, Respiratory syncytial virus",
author = "Linssen, {Rosalie S. N.} and Adithya Sridhar and Giulia Moreni and {van der Wel}, {Nicole N.} and {van Woensel}, {Job B. M.} and Wolthers, {Katja C.} and Bem, {Reinout A.}",
note = "Funding Information: Funding: Rosalie Linssen received an Amsterdam UMC PhD Graduate School scholarship. This study was further supported by Ars Donandi, CJ Vaillant Foundation and Janivo Foundation. Giulia Moreni was supported by OrganoVIR (grant 812673). Publisher Copyright: {\textcopyright} 2022 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2022",
month = mar,
day = "1",
doi = "10.3390/cells11050785",
language = "English",
volume = "11",
journal = "Cells",
issn = "2073-4409",
publisher = "MDPI Multidisciplinary Digital Publishing Institute",
number = "5",

}

RIS

TY - JOUR

T1 - Neutrophil Extracellular Traps Do Not Induce Injury and Inflammation in Well-Differentiated RSV-Infected Airway Epithelium

AU - Linssen, Rosalie S. N.

AU - Sridhar, Adithya

AU - Moreni, Giulia

AU - van der Wel, Nicole N.

AU - van Woensel, Job B. M.

AU - Wolthers, Katja C.

AU - Bem, Reinout A.

N1 - Funding Information: Funding: Rosalie Linssen received an Amsterdam UMC PhD Graduate School scholarship. This study was further supported by Ars Donandi, CJ Vaillant Foundation and Janivo Foundation. Giulia Moreni was supported by OrganoVIR (grant 812673). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2022/3/1

Y1 - 2022/3/1

N2 - Respiratory syncytial virus (RSV) lower respiratory tract infection (LRTI) causes a major burden of disease. The host response in RSV-LRTI is characterized by airway epithelial injury, inflammation and neutrophil influx, with the formation of neutrophil extracellular traps (NETs). However, the precise role of NETs in the pathophysiology of RSV-LRTI remains to be elucidated. Here, we used well-differentiated human airway epithelial cultures (HAE) of a pediatric and adult donor to study whether NETs cause airway epithelial injury and inflammation in the setting of RSV infection. The exposure of uninfected and RSV-infected HAE cultures to NETs, as produced by stimulation of neutrophils by a low dose of phorbol 12-myristate 13-acetate (PMA), did not induce or aggravate cell injury or inflammation. RSV infection of HAE cultures caused release of pro-inflammatory cytokines such as IL-6 and RANTES in both adult and pediatric cultures, but the differential gene expression for regulated cell death differed between culture donors. In this in vitro airway epithelial model, NETs in the setting of RSV infection did not cause or aggravate epithelial injury or inflammation.

AB - Respiratory syncytial virus (RSV) lower respiratory tract infection (LRTI) causes a major burden of disease. The host response in RSV-LRTI is characterized by airway epithelial injury, inflammation and neutrophil influx, with the formation of neutrophil extracellular traps (NETs). However, the precise role of NETs in the pathophysiology of RSV-LRTI remains to be elucidated. Here, we used well-differentiated human airway epithelial cultures (HAE) of a pediatric and adult donor to study whether NETs cause airway epithelial injury and inflammation in the setting of RSV infection. The exposure of uninfected and RSV-infected HAE cultures to NETs, as produced by stimulation of neutrophils by a low dose of phorbol 12-myristate 13-acetate (PMA), did not induce or aggravate cell injury or inflammation. RSV infection of HAE cultures caused release of pro-inflammatory cytokines such as IL-6 and RANTES in both adult and pediatric cultures, but the differential gene expression for regulated cell death differed between culture donors. In this in vitro airway epithelial model, NETs in the setting of RSV infection did not cause or aggravate epithelial injury or inflammation.

KW - Cell culture

KW - Human airway epithelial

KW - Infection

KW - Neutrophil extracellular traps

KW - Neutrophils

KW - RSV

KW - Respiratory syncytial virus

UR - http://www.scopus.com/inward/record.url?scp=85125231152&partnerID=8YFLogxK

U2 - 10.3390/cells11050785

DO - 10.3390/cells11050785

M3 - Article

C2 - 35269407

VL - 11

JO - Cells

JF - Cells

SN - 2073-4409

IS - 5

M1 - 785

ER -

ID: 22005279