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Multiplexed detection and differentiation of bacterial enzymes and bacteria by color-encoded sensor hydrogels. / Jia, Zhiyuan; Müller, Mareike; le Gall, Tony et al.

In: Bioactive Materials, Vol. 6, No. 12, 01.12.2021, p. 4286-4300.

Research output: Contribution to journalArticleAcademicpeer-review

Harvard

Jia, Z, Müller, M, le Gall, T, Riool, M, Müller, M, Zaat, SAJ, Montier, T & Schönherr, H 2021, 'Multiplexed detection and differentiation of bacterial enzymes and bacteria by color-encoded sensor hydrogels', Bioactive Materials, vol. 6, no. 12, pp. 4286-4300. https://doi.org/10.1016/j.bioactmat.2021.04.022

APA

Jia, Z., Müller, M., le Gall, T., Riool, M., Müller, M., Zaat, S. A. J., Montier, T., & Schönherr, H. (2021). Multiplexed detection and differentiation of bacterial enzymes and bacteria by color-encoded sensor hydrogels. Bioactive Materials, 6(12), 4286-4300. https://doi.org/10.1016/j.bioactmat.2021.04.022

Vancouver

Jia Z, Müller M, le Gall T, Riool M, Müller M, Zaat SAJ et al. Multiplexed detection and differentiation of bacterial enzymes and bacteria by color-encoded sensor hydrogels. Bioactive Materials. 2021 Dec 1;6(12):4286-4300. doi: 10.1016/j.bioactmat.2021.04.022

Author

Jia, Zhiyuan ; Müller, Mareike ; le Gall, Tony et al. / Multiplexed detection and differentiation of bacterial enzymes and bacteria by color-encoded sensor hydrogels. In: Bioactive Materials. 2021 ; Vol. 6, No. 12. pp. 4286-4300.

BibTeX

@article{ac61710e91904d9c9bbf210c82fe9e5a,
title = "Multiplexed detection and differentiation of bacterial enzymes and bacteria by color-encoded sensor hydrogels",
abstract = "We report on the fabrication and characterization of color-encoded chitosan hydrogels for the rapid, sensitive and specific detection of bacterial enzymes as well as the selective detection of a set of tested bacteria through characteristic enzyme reactions. These patterned sensor hydrogels are functionalized with three different colorimetric enzyme substrates affording the multiplexed detection and differentiation of α-glucosidase, β-galactosidase and β-glucuronidase. The limits of detection of the hydrogels for an observation time of 60 min using a conventional microplate reader correspond to concentrations of 0.2, 3.4 and 4.5 nM of these enzymes, respectively. Based on their different enzyme expression patterns, Staphylococcus aureus strain RN4220, methicillin-resistant S. aureus (MRSA) strain N315, both producing α-glucosidase, but not β-glucuronidase and β-galactosidase, Escherichia coli strain DH5α, producing β-glucuronidase and α-glucosidase, but not β-galactosidase, and the enterohemorrhagic E. coli (EHEC) strain E32511, producing β-galactosidase, but none of the other two enzymes, can be reliably and rapidly distinguished from each other. These results confirm the applicability of enzyme sensing hydrogels for the detection and discrimination of specific enzymes to facilitate differentiation of bacterial strains. Patterned hydrogels thus possess the potential to be further refined as detection units of a multiplexed format to identify certain bacteria for future application in point-of-care microbiological diagnostics in food safety and medical settings.",
keywords = "Bacteria detection, Bacterial enzyme, Colorimetric substrates, Multiplexed biosensors, Reporter hydrogels",
author = "Zhiyuan Jia and Mareike M{\"u}ller and {le Gall}, Tony and Martijn Riool and Max M{\"u}ller and Zaat, {Sebastian A. J.} and Tristan Montier and Holger Sch{\"o}nherr",
note = "Funding Information: This work was supported by the European Research Council (ERC grant to HS, grant No. 279202 ), the German Academic Exchange Service (DAAD) with financial support of the Bundesministerium f{\"u}r Bildung und Forschung (BMBF) , DAAD PPP Frankreich (Projekt-ID 55976814 ), the Equality Office of the University of Siegen , the Max-Buchner-Forschungsstiftung-Dechema (MBFSt-Kennziffer: 3671 ), Minist{\`e}re de l{\textquoteright}Europe et des Affaires Etrang{\`e}res (MEAE) and Minist{\`e}re de l{\textquoteright}Enseignement Sup{\'e}rieur, de la Recherche et de l{\textquoteright}Innovation (MESRI; PHC PROCOPE 2017, Project no. 37733UM ), SFR SCINBIOS/IBSAM (Brest - France) and association Gaetan Saleun (Brest - France) and the University of Siegen. Funding Information: This work was supported by the European Research Council (ERC grant to HS, grant No. 279202), the German Academic Exchange Service (DAAD) with financial support of the Bundesministerium f?r Bildung und Forschung (BMBF), DAAD PPP Frankreich (Projekt-ID 55976814), the Equality Office of the University of Siegen, the Max-Buchner-Forschungsstiftung-Dechema (MBFSt-Kennziffer: 3671), Minist?re de l'Europe et des Affaires Etrang?res (MEAE) and Minist?re de l'Enseignement Sup?rieur, de la Recherche et de l'Innovation (MESRI; PHC PROCOPE 2017, Project no. 37733UM), SFR SCINBIOS/IBSAM (Brest - France) and association Gaetan Saleun (Brest - France) and the University of Siegen. Publisher Copyright: {\textcopyright} 2021 The Authors Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2021",
month = dec,
day = "1",
doi = "10.1016/j.bioactmat.2021.04.022",
language = "English",
volume = "6",
pages = "4286--4300",
journal = "Bioactive Materials",
issn = "2452-199X",
publisher = "KeAi Publishing Communications Ltd.",
number = "12",

}

RIS

TY - JOUR

T1 - Multiplexed detection and differentiation of bacterial enzymes and bacteria by color-encoded sensor hydrogels

AU - Jia, Zhiyuan

AU - Müller, Mareike

AU - le Gall, Tony

AU - Riool, Martijn

AU - Müller, Max

AU - Zaat, Sebastian A. J.

AU - Montier, Tristan

AU - Schönherr, Holger

N1 - Funding Information: This work was supported by the European Research Council (ERC grant to HS, grant No. 279202 ), the German Academic Exchange Service (DAAD) with financial support of the Bundesministerium für Bildung und Forschung (BMBF) , DAAD PPP Frankreich (Projekt-ID 55976814 ), the Equality Office of the University of Siegen , the Max-Buchner-Forschungsstiftung-Dechema (MBFSt-Kennziffer: 3671 ), Ministère de l’Europe et des Affaires Etrangères (MEAE) and Ministère de l’Enseignement Supérieur, de la Recherche et de l’Innovation (MESRI; PHC PROCOPE 2017, Project no. 37733UM ), SFR SCINBIOS/IBSAM (Brest - France) and association Gaetan Saleun (Brest - France) and the University of Siegen. Funding Information: This work was supported by the European Research Council (ERC grant to HS, grant No. 279202), the German Academic Exchange Service (DAAD) with financial support of the Bundesministerium f?r Bildung und Forschung (BMBF), DAAD PPP Frankreich (Projekt-ID 55976814), the Equality Office of the University of Siegen, the Max-Buchner-Forschungsstiftung-Dechema (MBFSt-Kennziffer: 3671), Minist?re de l'Europe et des Affaires Etrang?res (MEAE) and Minist?re de l'Enseignement Sup?rieur, de la Recherche et de l'Innovation (MESRI; PHC PROCOPE 2017, Project no. 37733UM), SFR SCINBIOS/IBSAM (Brest - France) and association Gaetan Saleun (Brest - France) and the University of Siegen. Publisher Copyright: © 2021 The Authors Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/12/1

Y1 - 2021/12/1

N2 - We report on the fabrication and characterization of color-encoded chitosan hydrogels for the rapid, sensitive and specific detection of bacterial enzymes as well as the selective detection of a set of tested bacteria through characteristic enzyme reactions. These patterned sensor hydrogels are functionalized with three different colorimetric enzyme substrates affording the multiplexed detection and differentiation of α-glucosidase, β-galactosidase and β-glucuronidase. The limits of detection of the hydrogels for an observation time of 60 min using a conventional microplate reader correspond to concentrations of 0.2, 3.4 and 4.5 nM of these enzymes, respectively. Based on their different enzyme expression patterns, Staphylococcus aureus strain RN4220, methicillin-resistant S. aureus (MRSA) strain N315, both producing α-glucosidase, but not β-glucuronidase and β-galactosidase, Escherichia coli strain DH5α, producing β-glucuronidase and α-glucosidase, but not β-galactosidase, and the enterohemorrhagic E. coli (EHEC) strain E32511, producing β-galactosidase, but none of the other two enzymes, can be reliably and rapidly distinguished from each other. These results confirm the applicability of enzyme sensing hydrogels for the detection and discrimination of specific enzymes to facilitate differentiation of bacterial strains. Patterned hydrogels thus possess the potential to be further refined as detection units of a multiplexed format to identify certain bacteria for future application in point-of-care microbiological diagnostics in food safety and medical settings.

AB - We report on the fabrication and characterization of color-encoded chitosan hydrogels for the rapid, sensitive and specific detection of bacterial enzymes as well as the selective detection of a set of tested bacteria through characteristic enzyme reactions. These patterned sensor hydrogels are functionalized with three different colorimetric enzyme substrates affording the multiplexed detection and differentiation of α-glucosidase, β-galactosidase and β-glucuronidase. The limits of detection of the hydrogels for an observation time of 60 min using a conventional microplate reader correspond to concentrations of 0.2, 3.4 and 4.5 nM of these enzymes, respectively. Based on their different enzyme expression patterns, Staphylococcus aureus strain RN4220, methicillin-resistant S. aureus (MRSA) strain N315, both producing α-glucosidase, but not β-glucuronidase and β-galactosidase, Escherichia coli strain DH5α, producing β-glucuronidase and α-glucosidase, but not β-galactosidase, and the enterohemorrhagic E. coli (EHEC) strain E32511, producing β-galactosidase, but none of the other two enzymes, can be reliably and rapidly distinguished from each other. These results confirm the applicability of enzyme sensing hydrogels for the detection and discrimination of specific enzymes to facilitate differentiation of bacterial strains. Patterned hydrogels thus possess the potential to be further refined as detection units of a multiplexed format to identify certain bacteria for future application in point-of-care microbiological diagnostics in food safety and medical settings.

KW - Bacteria detection

KW - Bacterial enzyme

KW - Colorimetric substrates

KW - Multiplexed biosensors

KW - Reporter hydrogels

UR - http://www.scopus.com/inward/record.url?scp=85105305590&partnerID=8YFLogxK

U2 - 10.1016/j.bioactmat.2021.04.022

DO - 10.1016/j.bioactmat.2021.04.022

M3 - Article

C2 - 33997506

VL - 6

SP - 4286

EP - 4300

JO - Bioactive Materials

JF - Bioactive Materials

SN - 2452-199X

IS - 12

ER -

ID: 18159568