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Multicenter study to evaluate a new enumeration method for hematopoietic stem cell collection management. / Grommé, Monique; Russcher, Henk; Braakman, Eric et al.

In: Transfusion, Vol. 57, No. 8, 2017, p. 1949-1955.

Research output: Contribution to journalArticleAcademicpeer-review

Harvard

Grommé, M, Russcher, H, Braakman, E, Klinkspoor, JH, Dobber, JA, de Greef, I & de Wit, NCJ 2017, 'Multicenter study to evaluate a new enumeration method for hematopoietic stem cell collection management', Transfusion, vol. 57, no. 8, pp. 1949-1955. https://doi.org/10.1111/trf.14183

APA

Grommé, M., Russcher, H., Braakman, E., Klinkspoor, J. H., Dobber, J. A., de Greef, I., & de Wit, N. C. J. (2017). Multicenter study to evaluate a new enumeration method for hematopoietic stem cell collection management. Transfusion, 57(8), 1949-1955. https://doi.org/10.1111/trf.14183

Vancouver

Grommé M, Russcher H, Braakman E, Klinkspoor JH, Dobber JA, de Greef I et al. Multicenter study to evaluate a new enumeration method for hematopoietic stem cell collection management. Transfusion. 2017;57(8):1949-1955. Epub 2017. doi: 10.1111/trf.14183

Author

Grommé, Monique ; Russcher, Henk ; Braakman, Eric et al. / Multicenter study to evaluate a new enumeration method for hematopoietic stem cell collection management. In: Transfusion. 2017 ; Vol. 57, No. 8. pp. 1949-1955.

BibTeX

@article{5379095049c74bf69d920d32b2c25f4e,
title = "Multicenter study to evaluate a new enumeration method for hematopoietic stem cell collection management",
abstract = "CD34 flow cytometry is the gold standard for stem cell enumeration in peripheral blood at the mobilization stage and in the final apheresis product. The new stem cell mode of the Sysmex XN Series analyzer enumerates an immature cell population in the white progenitor and pathological cell (WPC) channel, based on the cell size, internal cellular complexity, and fluorescence intensity. In this multicenter study we analyzed 147 peripheral blood samples, 22 samples during collection of stem cells, and 45 samples from the apheresis product of 18 healthy allogeneic donors and 84 autologous patients. In this multicenter study we demonstrate that the XN stem cell enumeration method correlates well with viable CD34+ cells determined by flow cytometry during the stem cell mobilization phase to determine apheresis start time, during apheresis for real-time monitoring and adjustment, and for quality control of the final stem cell harvest. Our data show that there is an improvement in the correlation of XN stem cells and CD34+ cells in the peripheral blood during stem cell mobilization as well as in stem cell harvests compared to SE or XE Series analyzers. The XN stem cell enumeration method has a number of advantages compared to CD34 flow cytometry: it is fast, simple, reproducible, and less expensive. CE marking for the European market has been obtained, making the stem cell count on the XN analyzer a reportable clinical variable",
author = "Monique Gromm{\'e} and Henk Russcher and Eric Braakman and Klinkspoor, {J. Henri{\"e}tte} and Dobber, {Johan A.} and {de Greef}, Inge and {de Wit}, {Norbert C. J.}",
year = "2017",
doi = "10.1111/trf.14183",
language = "English",
volume = "57",
pages = "1949--1955",
journal = "Transfusion",
issn = "0041-1132",
publisher = "Wiley-Blackwell",
number = "8",

}

RIS

TY - JOUR

T1 - Multicenter study to evaluate a new enumeration method for hematopoietic stem cell collection management

AU - Grommé, Monique

AU - Russcher, Henk

AU - Braakman, Eric

AU - Klinkspoor, J. Henriëtte

AU - Dobber, Johan A.

AU - de Greef, Inge

AU - de Wit, Norbert C. J.

PY - 2017

Y1 - 2017

N2 - CD34 flow cytometry is the gold standard for stem cell enumeration in peripheral blood at the mobilization stage and in the final apheresis product. The new stem cell mode of the Sysmex XN Series analyzer enumerates an immature cell population in the white progenitor and pathological cell (WPC) channel, based on the cell size, internal cellular complexity, and fluorescence intensity. In this multicenter study we analyzed 147 peripheral blood samples, 22 samples during collection of stem cells, and 45 samples from the apheresis product of 18 healthy allogeneic donors and 84 autologous patients. In this multicenter study we demonstrate that the XN stem cell enumeration method correlates well with viable CD34+ cells determined by flow cytometry during the stem cell mobilization phase to determine apheresis start time, during apheresis for real-time monitoring and adjustment, and for quality control of the final stem cell harvest. Our data show that there is an improvement in the correlation of XN stem cells and CD34+ cells in the peripheral blood during stem cell mobilization as well as in stem cell harvests compared to SE or XE Series analyzers. The XN stem cell enumeration method has a number of advantages compared to CD34 flow cytometry: it is fast, simple, reproducible, and less expensive. CE marking for the European market has been obtained, making the stem cell count on the XN analyzer a reportable clinical variable

AB - CD34 flow cytometry is the gold standard for stem cell enumeration in peripheral blood at the mobilization stage and in the final apheresis product. The new stem cell mode of the Sysmex XN Series analyzer enumerates an immature cell population in the white progenitor and pathological cell (WPC) channel, based on the cell size, internal cellular complexity, and fluorescence intensity. In this multicenter study we analyzed 147 peripheral blood samples, 22 samples during collection of stem cells, and 45 samples from the apheresis product of 18 healthy allogeneic donors and 84 autologous patients. In this multicenter study we demonstrate that the XN stem cell enumeration method correlates well with viable CD34+ cells determined by flow cytometry during the stem cell mobilization phase to determine apheresis start time, during apheresis for real-time monitoring and adjustment, and for quality control of the final stem cell harvest. Our data show that there is an improvement in the correlation of XN stem cells and CD34+ cells in the peripheral blood during stem cell mobilization as well as in stem cell harvests compared to SE or XE Series analyzers. The XN stem cell enumeration method has a number of advantages compared to CD34 flow cytometry: it is fast, simple, reproducible, and less expensive. CE marking for the European market has been obtained, making the stem cell count on the XN analyzer a reportable clinical variable

U2 - 10.1111/trf.14183

DO - 10.1111/trf.14183

M3 - Article

C2 - 28653370

VL - 57

SP - 1949

EP - 1955

JO - Transfusion

JF - Transfusion

SN - 0041-1132

IS - 8

ER -

ID: 3936466