Standard

MiR-223-3p and miR-122-5p as circulating biomarkers for plaque instability. / Singh, Sandeep; de Ronde, Maurice W J; Kok, Maayke G M et al.

In: Open heart, Vol. 7, No. 1, e001223, 06.2020.

Research output: Contribution to journal › Article › Academic › peer-review

Harvard

Singh, S, de Ronde, MWJ, Kok, MGM, Beijk, MA, De Winter, RJ, van der Wal, AC, Sondermeijer, BM, Meijers, JCM, Creemers, EE & Pinto-Sietsma, S-J 2020, 'MiR-223-3p and miR-122-5p as circulating biomarkers for plaque instability', Open heart, vol. 7, no. 1, e001223. https://doi.org/10.1136/openhrt-2019-001223

APA

Singh, S., de Ronde, M. W. J., Kok, M. G. M., Beijk, M. A., De Winter, R. J., van der Wal, A. C., Sondermeijer, B. M., Meijers, J. C. M., Creemers, E. E., & Pinto-Sietsma, S-J. (2020). MiR-223-3p and miR-122-5p as circulating biomarkers for plaque instability. Open heart, 7(1), [e001223]. https://doi.org/10.1136/openhrt-2019-001223

Vancouver

Singh S, de Ronde MWJ, Kok MGM, Beijk MA, De Winter RJ, van der Wal AC et al. MiR-223-3p and miR-122-5p as circulating biomarkers for plaque instability. Open heart. 2020 Jun;7(1):e001223. doi: 10.1136/openhrt-2019-001223

Author

Singh, Sandeep ; de Ronde, Maurice W J ; Kok, Maayke G M et al. / MiR-223-3p and miR-122-5p as circulating biomarkers for plaque instability. In: Open heart. 2020 ; Vol. 7, No. 1.

BibTeX

@article{9ee4a9af63014e5dbfb8574f4d790d08,
title = "MiR-223-3p and miR-122-5p as circulating biomarkers for plaque instability",
abstract = "BACKGROUND: In this study, we discovered and validated candidate microRNA (miRNA) biomarkers for coronary artery disease (CAD).METHOD: Candidate tissue-derived miRNAs from atherosclerotic plaque material in patients with stable coronary artery disease (SCAD) (n=14) and unstable coronary artery disease (UCAD) (n=25) were discovered by qPCR-based arrays. We validated differentially expressed miRNAs, along with seven promising CAD-associated miRNAs from the literature, in the serum of two large cohorts (n=395 and n=1000) of patients with SCAD and UCAD and subclinical atherosclerosis (SubA) and controls, respectively.RESULT: From plaque materials (discovery phase), miR-125b-5p and miR-193b-3p were most upregulated in SCAD, whereas miR-223-3p and miR-142-3p were most upregulated in patients with UCAD. Subsequent validation in serum from patients with UCAD, SCAD, SubA and controls demonstrated significant upregulation of miR-223-3p, miR-133a-3p, miR-146-3p and miR-155-5p. The ischaemia-related miR-499-5p was also highly upregulated in patients with UCAD compared with the other groups (SCAD OR 20.63 (95% CI 11.16 to 38.15), SubA OR 96.10 (95% CI 40.13 to 230.14) and controls OR 15.73 (95% CI 7.80 to 31.72)). However, no significant difference in miR-499-5p expression was observed across SCAD, SubA and controls. MiR-122-5p was the only miRNA to be significantly upregulated in the serum of both patients with UCAD and SCAD.CONCLUSION: In conclusion, miR-122-5p and miR-223-3p might be markers of plaque instability.",
author = "Sandeep Singh and {de Ronde}, {Maurice W J} and Kok, {Maayke G M} and Beijk, {Marcel Am} and {De Winter}, {Robbert J} and {van der Wal}, {Allard C} and Sondermeijer, {Brigitte M} and Meijers, {Joost C M} and Creemers, {Esther E} and Sara-Joan Pinto-Sietsma",
note = "{\textcopyright} Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.",
year = "2020",
month = jun,
doi = "10.1136/openhrt-2019-001223",
language = "English",
volume = "7",
journal = "Open heart",
issn = "2398-595X",
publisher = "BMJ Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - MiR-223-3p and miR-122-5p as circulating biomarkers for plaque instability

AU - Singh, Sandeep

AU - de Ronde, Maurice W J

AU - Kok, Maayke G M

AU - Beijk, Marcel Am

AU - De Winter, Robbert J

AU - van der Wal, Allard C

AU - Sondermeijer, Brigitte M

AU - Meijers, Joost C M

AU - Creemers, Esther E

AU - Pinto-Sietsma, Sara-Joan

N1 - © Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

PY - 2020/6

Y1 - 2020/6

N2 - BACKGROUND: In this study, we discovered and validated candidate microRNA (miRNA) biomarkers for coronary artery disease (CAD).METHOD: Candidate tissue-derived miRNAs from atherosclerotic plaque material in patients with stable coronary artery disease (SCAD) (n=14) and unstable coronary artery disease (UCAD) (n=25) were discovered by qPCR-based arrays. We validated differentially expressed miRNAs, along with seven promising CAD-associated miRNAs from the literature, in the serum of two large cohorts (n=395 and n=1000) of patients with SCAD and UCAD and subclinical atherosclerosis (SubA) and controls, respectively.RESULT: From plaque materials (discovery phase), miR-125b-5p and miR-193b-3p were most upregulated in SCAD, whereas miR-223-3p and miR-142-3p were most upregulated in patients with UCAD. Subsequent validation in serum from patients with UCAD, SCAD, SubA and controls demonstrated significant upregulation of miR-223-3p, miR-133a-3p, miR-146-3p and miR-155-5p. The ischaemia-related miR-499-5p was also highly upregulated in patients with UCAD compared with the other groups (SCAD OR 20.63 (95% CI 11.16 to 38.15), SubA OR 96.10 (95% CI 40.13 to 230.14) and controls OR 15.73 (95% CI 7.80 to 31.72)). However, no significant difference in miR-499-5p expression was observed across SCAD, SubA and controls. MiR-122-5p was the only miRNA to be significantly upregulated in the serum of both patients with UCAD and SCAD.CONCLUSION: In conclusion, miR-122-5p and miR-223-3p might be markers of plaque instability.

AB - BACKGROUND: In this study, we discovered and validated candidate microRNA (miRNA) biomarkers for coronary artery disease (CAD).METHOD: Candidate tissue-derived miRNAs from atherosclerotic plaque material in patients with stable coronary artery disease (SCAD) (n=14) and unstable coronary artery disease (UCAD) (n=25) were discovered by qPCR-based arrays. We validated differentially expressed miRNAs, along with seven promising CAD-associated miRNAs from the literature, in the serum of two large cohorts (n=395 and n=1000) of patients with SCAD and UCAD and subclinical atherosclerosis (SubA) and controls, respectively.RESULT: From plaque materials (discovery phase), miR-125b-5p and miR-193b-3p were most upregulated in SCAD, whereas miR-223-3p and miR-142-3p were most upregulated in patients with UCAD. Subsequent validation in serum from patients with UCAD, SCAD, SubA and controls demonstrated significant upregulation of miR-223-3p, miR-133a-3p, miR-146-3p and miR-155-5p. The ischaemia-related miR-499-5p was also highly upregulated in patients with UCAD compared with the other groups (SCAD OR 20.63 (95% CI 11.16 to 38.15), SubA OR 96.10 (95% CI 40.13 to 230.14) and controls OR 15.73 (95% CI 7.80 to 31.72)). However, no significant difference in miR-499-5p expression was observed across SCAD, SubA and controls. MiR-122-5p was the only miRNA to be significantly upregulated in the serum of both patients with UCAD and SCAD.CONCLUSION: In conclusion, miR-122-5p and miR-223-3p might be markers of plaque instability.

U2 - 10.1136/openhrt-2019-001223

DO - 10.1136/openhrt-2019-001223

M3 - Article

C2 - 32487772

VL - 7

JO - Open heart

JF - Open heart

SN - 2398-595X

IS - 1

M1 - e001223

ER -

ID: 14291781