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Minimum information to report about a flow cytometry experiment on extracellular vesicles : Communication from the ISTH SSC subcommittee on vascular biology. / van der Pol, Edwin; Welsh, Joshua A.; Nieuwland, Rienk.

In: Journal of thrombosis and haemostasis, Vol. 20, No. 1, 01.2022, p. 245-251.

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@article{fb75a3c40abe4f5e992268f871caa67e,
title = "Minimum information to report about a flow cytometry experiment on extracellular vesicles: Communication from the ISTH SSC subcommittee on vascular biology",
abstract = "The Extracellular Vesicle Flow Cytometry Working Group (http://www.evflowcytometry.org) is formed by members of the International Society for Extracellular Vesicles (ISEV), the International Society for Advancement of Cytometry (ISAC), and the International Society on Thrombosis and Haemostasis (ISTH). This working group of flow cytometry experts develops guidelines for best practices regarding flow cytometry detection of extracellular vesicles. To improve rigor and standardization, this working group published a framework outlining the minimal information to report about a flow cytometry experiment on extracellular vesicles (MIFlowCyt-EV) in the Journal of Extracellular Vesicles, the ISEV journal, in 2020. In parallel, an article explaining MIFlowCyt-EV was published in Cytometry Part A, one of the ISAC journals, and now will be introduced to the ISTH as an SSC Communication in the Journal of Thrombosis and Haemostasis. The goal of this SSC Communication is to explain why flow cytometry is becoming the instrument of choice to characterize single extracellular vesicles, the obstacles that have been identified and (mostly) overcome by developing procedures to calibrate flow cytometers, and the relevance of reporting minimal information to improve reliability and reproducibility of experiments in which flow cytometers are used for characterization of extracellular vesicles.",
keywords = "calibration, extracellular vesicles, flow cytometry, reproducibility of results, research design",
author = "{van der Pol}, Edwin and Welsh, {Joshua A.} and Rienk Nieuwland",
note = "Funding Information: EvdP is funded by the Dutch Research Council (NWO), Grant/Award Number: VENI 15924. JAW is funded by the National Institutes of Health (NIH), Grant/Award Numbers: UG3 TR002881 and ZIA BC011502. RN is funded by the European Association of National Metrology Institutes, Grant/Award Number: 18HLT01 METVES II. Publisher Copyright: {\textcopyright} 2021 The Authors. Journal of Thrombosis and Haemostasis published by Wiley Periodicals LLC on behalf of International Society on Thrombosis and Haemostasis. This article has been contributed to by US Government employees and their work is in the public domain in the USA.",
year = "2022",
month = jan,
doi = "10.1111/jth.15540",
language = "English",
volume = "20",
pages = "245--251",
journal = "Journal of thrombosis and haemostasis",
issn = "1538-7933",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Minimum information to report about a flow cytometry experiment on extracellular vesicles

T2 - Communication from the ISTH SSC subcommittee on vascular biology

AU - van der Pol, Edwin

AU - Welsh, Joshua A.

AU - Nieuwland, Rienk

N1 - Funding Information: EvdP is funded by the Dutch Research Council (NWO), Grant/Award Number: VENI 15924. JAW is funded by the National Institutes of Health (NIH), Grant/Award Numbers: UG3 TR002881 and ZIA BC011502. RN is funded by the European Association of National Metrology Institutes, Grant/Award Number: 18HLT01 METVES II. Publisher Copyright: © 2021 The Authors. Journal of Thrombosis and Haemostasis published by Wiley Periodicals LLC on behalf of International Society on Thrombosis and Haemostasis. This article has been contributed to by US Government employees and their work is in the public domain in the USA.

PY - 2022/1

Y1 - 2022/1

N2 - The Extracellular Vesicle Flow Cytometry Working Group (http://www.evflowcytometry.org) is formed by members of the International Society for Extracellular Vesicles (ISEV), the International Society for Advancement of Cytometry (ISAC), and the International Society on Thrombosis and Haemostasis (ISTH). This working group of flow cytometry experts develops guidelines for best practices regarding flow cytometry detection of extracellular vesicles. To improve rigor and standardization, this working group published a framework outlining the minimal information to report about a flow cytometry experiment on extracellular vesicles (MIFlowCyt-EV) in the Journal of Extracellular Vesicles, the ISEV journal, in 2020. In parallel, an article explaining MIFlowCyt-EV was published in Cytometry Part A, one of the ISAC journals, and now will be introduced to the ISTH as an SSC Communication in the Journal of Thrombosis and Haemostasis. The goal of this SSC Communication is to explain why flow cytometry is becoming the instrument of choice to characterize single extracellular vesicles, the obstacles that have been identified and (mostly) overcome by developing procedures to calibrate flow cytometers, and the relevance of reporting minimal information to improve reliability and reproducibility of experiments in which flow cytometers are used for characterization of extracellular vesicles.

AB - The Extracellular Vesicle Flow Cytometry Working Group (http://www.evflowcytometry.org) is formed by members of the International Society for Extracellular Vesicles (ISEV), the International Society for Advancement of Cytometry (ISAC), and the International Society on Thrombosis and Haemostasis (ISTH). This working group of flow cytometry experts develops guidelines for best practices regarding flow cytometry detection of extracellular vesicles. To improve rigor and standardization, this working group published a framework outlining the minimal information to report about a flow cytometry experiment on extracellular vesicles (MIFlowCyt-EV) in the Journal of Extracellular Vesicles, the ISEV journal, in 2020. In parallel, an article explaining MIFlowCyt-EV was published in Cytometry Part A, one of the ISAC journals, and now will be introduced to the ISTH as an SSC Communication in the Journal of Thrombosis and Haemostasis. The goal of this SSC Communication is to explain why flow cytometry is becoming the instrument of choice to characterize single extracellular vesicles, the obstacles that have been identified and (mostly) overcome by developing procedures to calibrate flow cytometers, and the relevance of reporting minimal information to improve reliability and reproducibility of experiments in which flow cytometers are used for characterization of extracellular vesicles.

KW - calibration

KW - extracellular vesicles

KW - flow cytometry

KW - reproducibility of results

KW - research design

UR - http://www.scopus.com/inward/record.url?scp=85119975667&partnerID=8YFLogxK

U2 - 10.1111/jth.15540

DO - 10.1111/jth.15540

M3 - Article

C2 - 34637195

VL - 20

SP - 245

EP - 251

JO - Journal of thrombosis and haemostasis

JF - Journal of thrombosis and haemostasis

SN - 1538-7933

IS - 1

ER -

ID: 20633405