Local irradiation of patient-derived tumors in immunodeficient mice

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Local irradiation of patient-derived tumors in immunodeficient mice. / van der Zalm, Amber P.; Bootsma, Sanne; Rodermond, Hans M. et al.

In: STAR Protocols, Vol. 4, No. 1, 102098, 17.03.2023.

Research output: Contribution to journal › Article › Academic › peer-review

BibTeX

@article{d5d37e02a6a148efa486e3cc44bfe087,

title = "Local irradiation of patient-derived tumors in immunodeficient mice",

abstract = "Severe combined immunodeficient mice are typically used for xenografting experiments and show reliable tumor engraftment; however, their Prkd scid mutation renders them highly sensitive to irradiation. Here, we describe a protocol that allows safe local irradiation of tumor xenografts in immunodeficient mice. We detail the steps for the establishment and handling of patient-derived cancer cultures, subcutaneous injection of cancer cells on the mouse hind limb, localized irradiation in mice, tumor monitoring, and tumor characterization via histological and immunohistochemical assessment. For complete details on the use and execution of this protocol, please refer to Dings et al. (2022).",

keywords = "Cancer, Cell Culture, Health Sciences, Microscopy, Model Organisms",

author = "{van der Zalm}, {Amber P.} and Sanne Bootsma and Rodermond, {Hans M.} and Oei, {Arlene L.} and Bijlsma, {Maarten F.}",

note = "Funding Information: M.F.B. has received research funding from Celgene, Frame Therapeutics, and Lead Pharma and has acted as a consultant to Servier. Funding Information: This work was supported by KWF Dutch Cancer Society to M.F.B. (project number 10992 ). We would like to thank Cynthia Waasdorp, Marieke ten Brink, and the staff of the animal facility for technical assistance. We would like to acknowledge Eric van der Zalm and Gerbrand de Jong for creating the technical drawing and providing the files of the mice radiation setup. Publisher Copyright: {\textcopyright} 2023 The Author(s)",

year = "2023",

month = mar,

day = "17",

doi = "10.1016/j.xpro.2023.102098",

language = "English",

volume = "4",

journal = "STAR Protocols",

issn = "2666-1667",

publisher = "Cell Press",

number = "1",

}

RIS

TY - JOUR

T1 - Local irradiation of patient-derived tumors in immunodeficient mice

AU - van der Zalm, Amber P.

AU - Bootsma, Sanne

AU - Rodermond, Hans M.

AU - Oei, Arlene L.

AU - Bijlsma, Maarten F.

N1 - Funding Information: M.F.B. has received research funding from Celgene, Frame Therapeutics, and Lead Pharma and has acted as a consultant to Servier. Funding Information: This work was supported by KWF Dutch Cancer Society to M.F.B. (project number 10992 ). We would like to thank Cynthia Waasdorp, Marieke ten Brink, and the staff of the animal facility for technical assistance. We would like to acknowledge Eric van der Zalm and Gerbrand de Jong for creating the technical drawing and providing the files of the mice radiation setup. Publisher Copyright: © 2023 The Author(s)

PY - 2023/3/17

Y1 - 2023/3/17

N2 - Severe combined immunodeficient mice are typically used for xenografting experiments and show reliable tumor engraftment; however, their Prkd scid mutation renders them highly sensitive to irradiation. Here, we describe a protocol that allows safe local irradiation of tumor xenografts in immunodeficient mice. We detail the steps for the establishment and handling of patient-derived cancer cultures, subcutaneous injection of cancer cells on the mouse hind limb, localized irradiation in mice, tumor monitoring, and tumor characterization via histological and immunohistochemical assessment. For complete details on the use and execution of this protocol, please refer to Dings et al. (2022).

AB - Severe combined immunodeficient mice are typically used for xenografting experiments and show reliable tumor engraftment; however, their Prkd scid mutation renders them highly sensitive to irradiation. Here, we describe a protocol that allows safe local irradiation of tumor xenografts in immunodeficient mice. We detail the steps for the establishment and handling of patient-derived cancer cultures, subcutaneous injection of cancer cells on the mouse hind limb, localized irradiation in mice, tumor monitoring, and tumor characterization via histological and immunohistochemical assessment. For complete details on the use and execution of this protocol, please refer to Dings et al. (2022).

KW - Cancer

KW - Cell Culture

KW - Health Sciences

KW - Microscopy

KW - Model Organisms

UR - http://www.scopus.com/inward/record.url?scp=85147594713&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2023.102098

DO - 10.1016/j.xpro.2023.102098

M3 - Article

C2 - 36825807

VL - 4

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 1

M1 - 102098

ER -

ID: 32889656

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