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In vitro cytotoxicity evaluation of porous TiO2-Ag antibacterial coatings for human fetal osteoblasts. / Necula, B. S.; van Leeuwen, J. P. T. M.; Fratila-Apachitei, L. E. et al.

In: Acta biomaterialia, Vol. 8, No. 11, 2012, p. 4191-4197.

Research output: Contribution to journal › Article › Academic › peer-review

Harvard

Necula, BS, van Leeuwen, JPTM, Fratila-Apachitei, LE, Zaat, SAJ, Apachitei, I & Duszczyk, J 2012, 'In vitro cytotoxicity evaluation of porous TiO2-Ag antibacterial coatings for human fetal osteoblasts', Acta biomaterialia, vol. 8, no. 11, pp. 4191-4197. https://doi.org/10.1016/j.actbio.2012.07.005

APA

Necula, B. S., van Leeuwen, J. P. T. M., Fratila-Apachitei, L. E., Zaat, S. A. J., Apachitei, I., & Duszczyk, J. (2012). In vitro cytotoxicity evaluation of porous TiO2-Ag antibacterial coatings for human fetal osteoblasts. Acta biomaterialia, 8(11), 4191-4197. https://doi.org/10.1016/j.actbio.2012.07.005

Vancouver

Necula BS, van Leeuwen JPTM, Fratila-Apachitei LE, Zaat SAJ, Apachitei I, Duszczyk J. In vitro cytotoxicity evaluation of porous TiO2-Ag antibacterial coatings for human fetal osteoblasts. Acta biomaterialia. 2012;8(11):4191-4197. doi: 10.1016/j.actbio.2012.07.005

Author

Necula, B. S. ; van Leeuwen, J. P. T. M. ; Fratila-Apachitei, L. E. et al. / In vitro cytotoxicity evaluation of porous TiO2-Ag antibacterial coatings for human fetal osteoblasts. In: Acta biomaterialia. 2012 ; Vol. 8, No. 11. pp. 4191-4197.

BibTeX

@article{6b5319e199a942c08761661f46ddead0,
title = "In vitro cytotoxicity evaluation of porous TiO2-Ag antibacterial coatings for human fetal osteoblasts",
abstract = "Implant-associated infections (IAIs) may be prevented by providing antibacterial properties to the implant surface prior to implantation. Using a plasma electrolytic oxidation (PEO) technique, we produced porous TiO2 coatings bearing various concentrations of Ag nanoparticles (Ag NPs) (designated as Ag, 0.3 Ag and 3.0 Ag) on a Ti-6Al-7Nb biomedical alloy. This study investigates the cytotoxicity of these coatings using a human osteoblastic cell line (SV-HFO) and evaluates their bactericidal activity against methicillin-resistant Staphylococcus aureus (MRSA). The release of Ag and the total amount of Ag in the coatings were determined using a graphite furnace atomic absorption spectrometry technique (GF-AAS) and flame-AAS, respectively. Cytotoxicity was evaluated using the AlamarBlue assay coupled with the scanning electron microscopy (SEM) observation of seeded cells and by fluorescence microscopy examination of the actin cytoskeleton and nuclei after 48 h of incubation. Antibacterial activity was assessed quantitatively using a direct contact assay. AlamarBlue viability assay, SEM and fluorescence microscopy observation of the SV-HFO cells showed no toxicity for 0 Ag and 0.3 Ag specimens, after 2, 5 and 7 days of culture, while 3.0 Ag surfaces appeared to be extremely cytotoxic. All Ag-bearing surfaces had good antibacterial activity, whereas Ag-free coatings showed an increase in bacterial numbers. Our results show that the 0.3 Ag coatings offer conditions for optimum cell growth next to antibacterial properties, which makes them extremely useful for the development of new antibacterial dental and orthopedic implants. (C) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved",
author = "Necula, {B. S.} and {van Leeuwen}, {J. P. T. M.} and Fratila-Apachitei, {L. E.} and Zaat, {S. A. J.} and I. Apachitei and J. Duszczyk",
year = "2012",
doi = "10.1016/j.actbio.2012.07.005",
language = "English",
volume = "8",
pages = "4191--4197",
journal = "Acta biomaterialia",
issn = "1742-7061",
publisher = "Elsevier BV",
number = "11",

}

RIS

TY - JOUR

T1 - In vitro cytotoxicity evaluation of porous TiO2-Ag antibacterial coatings for human fetal osteoblasts

AU - Necula, B. S.

AU - van Leeuwen, J. P. T. M.

AU - Fratila-Apachitei, L. E.

AU - Zaat, S. A. J.

AU - Apachitei, I.

AU - Duszczyk, J.

PY - 2012

Y1 - 2012

N2 - Implant-associated infections (IAIs) may be prevented by providing antibacterial properties to the implant surface prior to implantation. Using a plasma electrolytic oxidation (PEO) technique, we produced porous TiO2 coatings bearing various concentrations of Ag nanoparticles (Ag NPs) (designated as Ag, 0.3 Ag and 3.0 Ag) on a Ti-6Al-7Nb biomedical alloy. This study investigates the cytotoxicity of these coatings using a human osteoblastic cell line (SV-HFO) and evaluates their bactericidal activity against methicillin-resistant Staphylococcus aureus (MRSA). The release of Ag and the total amount of Ag in the coatings were determined using a graphite furnace atomic absorption spectrometry technique (GF-AAS) and flame-AAS, respectively. Cytotoxicity was evaluated using the AlamarBlue assay coupled with the scanning electron microscopy (SEM) observation of seeded cells and by fluorescence microscopy examination of the actin cytoskeleton and nuclei after 48 h of incubation. Antibacterial activity was assessed quantitatively using a direct contact assay. AlamarBlue viability assay, SEM and fluorescence microscopy observation of the SV-HFO cells showed no toxicity for 0 Ag and 0.3 Ag specimens, after 2, 5 and 7 days of culture, while 3.0 Ag surfaces appeared to be extremely cytotoxic. All Ag-bearing surfaces had good antibacterial activity, whereas Ag-free coatings showed an increase in bacterial numbers. Our results show that the 0.3 Ag coatings offer conditions for optimum cell growth next to antibacterial properties, which makes them extremely useful for the development of new antibacterial dental and orthopedic implants. (C) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved

AB - Implant-associated infections (IAIs) may be prevented by providing antibacterial properties to the implant surface prior to implantation. Using a plasma electrolytic oxidation (PEO) technique, we produced porous TiO2 coatings bearing various concentrations of Ag nanoparticles (Ag NPs) (designated as Ag, 0.3 Ag and 3.0 Ag) on a Ti-6Al-7Nb biomedical alloy. This study investigates the cytotoxicity of these coatings using a human osteoblastic cell line (SV-HFO) and evaluates their bactericidal activity against methicillin-resistant Staphylococcus aureus (MRSA). The release of Ag and the total amount of Ag in the coatings were determined using a graphite furnace atomic absorption spectrometry technique (GF-AAS) and flame-AAS, respectively. Cytotoxicity was evaluated using the AlamarBlue assay coupled with the scanning electron microscopy (SEM) observation of seeded cells and by fluorescence microscopy examination of the actin cytoskeleton and nuclei after 48 h of incubation. Antibacterial activity was assessed quantitatively using a direct contact assay. AlamarBlue viability assay, SEM and fluorescence microscopy observation of the SV-HFO cells showed no toxicity for 0 Ag and 0.3 Ag specimens, after 2, 5 and 7 days of culture, while 3.0 Ag surfaces appeared to be extremely cytotoxic. All Ag-bearing surfaces had good antibacterial activity, whereas Ag-free coatings showed an increase in bacterial numbers. Our results show that the 0.3 Ag coatings offer conditions for optimum cell growth next to antibacterial properties, which makes them extremely useful for the development of new antibacterial dental and orthopedic implants. (C) 2012 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved

U2 - 10.1016/j.actbio.2012.07.005

DO - 10.1016/j.actbio.2012.07.005

M3 - Article

C2 - 22813846

VL - 8

SP - 4191

EP - 4197

JO - Acta biomaterialia

JF - Acta biomaterialia

SN - 1742-7061

IS - 11

ER -

ID: 1772703