Research output: Contribution to journal › Article › Academic › peer-review
Immunophenotypic analysis of the chronological events of tissue repair in aortic medial dissections. / Visonà, Silvia D.; de Boer, Onno J.; Mackaaij, Claire et al.
In: Cardiovascular pathology, Vol. 34, 2018, p. 9-14.Research output: Contribution to journal › Article › Academic › peer-review
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TY - JOUR
T1 - Immunophenotypic analysis of the chronological events of tissue repair in aortic medial dissections
AU - Visonà, Silvia D.
AU - de Boer, Onno J.
AU - Mackaaij, Claire
AU - de Boer, Hans H.
AU - Pertiwi, Kartika R.
AU - de Winter, Ruben W.
AU - Osculati, Antonio
AU - van der Wal, Allard C.
PY - 2018
Y1 - 2018
N2 - Acute medial dissection of aorta can occur in the context of a sudden and unexpected death. For medico-legal reasons it is important to estimate as accurately the histological age of dissections. We evaluated the additional value of a systematic application of immunohistochemistry, compared with conventional histology only, in determining chronological steps of injury and repair. Thirty two paraffin embedded specimens of aortic dissection were retrospectively allocated to one of four defined stages: acute (I), subacute (II), early organizing (III) and scarring (IV) using Hematoxylin and Eosin and Elastica van Gieson stained sections. Subsequent immunohistochemically staining was performed with the following markers: (myeloperoxidase (neutrophils), citrullinated-Histone 3 (neutrophil extracellular traps), CD68 (macrophages), CD3 (T-cells), CD31 and CD34 (endothelial cells), and smooth muscle actin. Immune stained sections were scored semi-quantitatively. Histologically, five cases were identified as stage I, 16 as II, 7 as III and 4 as IV. Additional immunostaining for smooth muscle cells and endothelial cells altered the classification in 25% of cases (all in groups II and III). Immunostaining and semi-quantitative grading of involvement of neutrophils, macrophages and NETs also provided specific distribution patterns over the 4 age categories, including unexpected involvement of the peri adventitial fat tissue. In conclusion, it appears that semi-quantitative immunohistochemistry of resident vascular wall cells, inflammatory cells and NETS represents a useful adjunct in detailed histopathological grading of the chronological age of aortic dissections.
AB - Acute medial dissection of aorta can occur in the context of a sudden and unexpected death. For medico-legal reasons it is important to estimate as accurately the histological age of dissections. We evaluated the additional value of a systematic application of immunohistochemistry, compared with conventional histology only, in determining chronological steps of injury and repair. Thirty two paraffin embedded specimens of aortic dissection were retrospectively allocated to one of four defined stages: acute (I), subacute (II), early organizing (III) and scarring (IV) using Hematoxylin and Eosin and Elastica van Gieson stained sections. Subsequent immunohistochemically staining was performed with the following markers: (myeloperoxidase (neutrophils), citrullinated-Histone 3 (neutrophil extracellular traps), CD68 (macrophages), CD3 (T-cells), CD31 and CD34 (endothelial cells), and smooth muscle actin. Immune stained sections were scored semi-quantitatively. Histologically, five cases were identified as stage I, 16 as II, 7 as III and 4 as IV. Additional immunostaining for smooth muscle cells and endothelial cells altered the classification in 25% of cases (all in groups II and III). Immunostaining and semi-quantitative grading of involvement of neutrophils, macrophages and NETs also provided specific distribution patterns over the 4 age categories, including unexpected involvement of the peri adventitial fat tissue. In conclusion, it appears that semi-quantitative immunohistochemistry of resident vascular wall cells, inflammatory cells and NETS represents a useful adjunct in detailed histopathological grading of the chronological age of aortic dissections.
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85043331074&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/29525729
U2 - 10.1016/j.carpath.2018.01.009
DO - 10.1016/j.carpath.2018.01.009
M3 - Article
C2 - 29525729
VL - 34
SP - 9
EP - 14
JO - Cardiovascular pathology
JF - Cardiovascular pathology
SN - 1054-8807
ER -
ID: 5487447