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Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential. / Otto, Iris A.; Bernal, Paulina Nuñez; Rikkers, Margot; van Rijen, Mattie H. P.; Mensinga, Anneloes; Kon, Moshe; Breugem, Corstiaan C.; Levato, Riccardo; Malda, Jos.

In: iScience, Vol. 25, No. 9, 104979, 16.09.2022.

Research output: Contribution to journalArticleAcademicpeer-review

Harvard

Otto, IA, Bernal, PN, Rikkers, M, van Rijen, MHP, Mensinga, A, Kon, M, Breugem, CC, Levato, R & Malda, J 2022, 'Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential', iScience, vol. 25, no. 9, 104979. https://doi.org/10.1016/j.isci.2022.104979

APA

Otto, I. A., Bernal, P. N., Rikkers, M., van Rijen, M. H. P., Mensinga, A., Kon, M., Breugem, C. C., Levato, R., & Malda, J. (2022). Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential. iScience, 25(9), [104979]. https://doi.org/10.1016/j.isci.2022.104979

Vancouver

Author

Otto, Iris A. ; Bernal, Paulina Nuñez ; Rikkers, Margot ; van Rijen, Mattie H. P. ; Mensinga, Anneloes ; Kon, Moshe ; Breugem, Corstiaan C. ; Levato, Riccardo ; Malda, Jos. / Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential. In: iScience. 2022 ; Vol. 25, No. 9.

BibTeX

@article{d4a05b59fd9a4c28bc355daecbce7f5d,
title = "Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential",
abstract = "Remaining challenges in auricular cartilage tissue engineering include acquiring sufficient amounts of regeneration-competent cells and subsequent production of high-quality neocartilage. Progenitor cells are a resident subpopulation of native cartilage, displaying a high proliferative and cartilage-forming capacity, yet their potential for regenerative medicine is vastly understudied. In this study, human auricular cartilage progenitor cells were newly identified in healthy cartilage and, importantly, in microtia-impaired chondral remnants. Their cartilage repair potential was assessed via in vitro 3D culture upon encapsulation in a gelatin-based hydrogel, and subsequent biochemical, mechanical, and histological analyses. Auricular cartilage progenitor cells demonstrate a potent ability to proliferate without losing their multipotent differentiation ability and to produce cartilage-like matrix in 3D culture. As these cells can be easily obtained through a non-deforming biopsy of the healthy ear or from the otherwise redundant microtia remnant, they can provide an important solution for long-existing challenges in auricular cartilage tissue engineering.",
author = "Otto, {Iris A.} and Bernal, {Paulina Nu{\~n}ez} and Margot Rikkers and {van Rijen}, {Mattie H. P.} and Anneloes Mensinga and Moshe Kon and Breugem, {Corstiaan C.} and Riccardo Levato and Jos Malda",
note = "Funding Information: All tissues were obtained from biopsies of redundant tissue excised during surgery or from deceased donors who had donated their body to science, according to the guidelines of the Ethical Committee of the University Medical Center Utrecht. The authors would like to thank prof. R.L.A.W. Bleys and S. Plomp of the Department of Anatomy and Dr. L.N.A. van Adrichem of the Department of Plastic, Reconstructive and Hand Surgery of the University Medical Center Utrecht for kindly providing tissues from deceased donors and otoplasty remnants, respectively. The authors also gratefully acknowledge Iris Pennings for developing the protocol for the staining of elastin, and thank Nasim Golafshan for help with the graphical abstract. The antibody against collagen type II, developed by T.F. Linsenmayer, was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242, USA. The research was supported by the Netherlands Organization for Scientific Research (Graduate Program Grant 022.005.018), the Dutch Arthritis Foundation (CO-14-001, LLP-12 and LLP-22), and the European Research Council under grant agreement No. 647426 (3D-JOINT). Conceptualization by I.A.O. M.K. C.C.B. R.L. and J.M.; Methodology by I.A.O. and R.L.; Investigation by I.A.O. P.N.B. M.R. M.H.P.R. and A.M.; Formal Analysis by I.A.O. P.N.B. and M.R.; Writing of the original draft by I.A.O.; Review & Editing by I.A.O. P.N.B. R.L. and J.M.; Resources & Visualization by I.A.O. and P.N.B.; Project Administration by I.A.O.; Supervision by M.K. C.C.B. R.L. and J.M. The authors declare no competing interests. Funding Information: All tissues were obtained from biopsies of redundant tissue excised during surgery or from deceased donors who had donated their body to science, according to the guidelines of the Ethical Committee of the University Medical Center Utrecht. The authors would like to thank prof. R.L.A.W. Bleys and S. Plomp of the Department of Anatomy and Dr. L.N.A. van Adrichem of the Department of Plastic, Reconstructive and Hand Surgery of the University Medical Center Utrecht for kindly providing tissues from deceased donors and otoplasty remnants, respectively. The authors also gratefully acknowledge Iris Pennings for developing the protocol for the staining of elastin, and thank Nasim Golafshan for help with the graphical abstract. The antibody against collagen type II, developed by T.F. Linsenmayer, was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242, USA. The research was supported by the Netherlands Organization for Scientific Research (Graduate Program Grant 022.005.018 ), the Dutch Arthritis Foundation ( CO-14-001 , LLP-12 and LLP-22 ), and the European Research Council under grant agreement No. 647426 (3D-JOINT). Publisher Copyright: {\textcopyright} 2022",
year = "2022",
month = sep,
day = "16",
doi = "10.1016/j.isci.2022.104979",
language = "English",
volume = "25",
journal = "iScience",
issn = "2589-0042",
publisher = "Elsevier Inc.",
number = "9",

}

RIS

TY - JOUR

T1 - Human adult, pediatric and microtia auricular cartilage harbor fibronectin-adhering progenitor cells with regenerative ear reconstruction potential

AU - Otto, Iris A.

AU - Bernal, Paulina Nuñez

AU - Rikkers, Margot

AU - van Rijen, Mattie H. P.

AU - Mensinga, Anneloes

AU - Kon, Moshe

AU - Breugem, Corstiaan C.

AU - Levato, Riccardo

AU - Malda, Jos

N1 - Funding Information: All tissues were obtained from biopsies of redundant tissue excised during surgery or from deceased donors who had donated their body to science, according to the guidelines of the Ethical Committee of the University Medical Center Utrecht. The authors would like to thank prof. R.L.A.W. Bleys and S. Plomp of the Department of Anatomy and Dr. L.N.A. van Adrichem of the Department of Plastic, Reconstructive and Hand Surgery of the University Medical Center Utrecht for kindly providing tissues from deceased donors and otoplasty remnants, respectively. The authors also gratefully acknowledge Iris Pennings for developing the protocol for the staining of elastin, and thank Nasim Golafshan for help with the graphical abstract. The antibody against collagen type II, developed by T.F. Linsenmayer, was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242, USA. The research was supported by the Netherlands Organization for Scientific Research (Graduate Program Grant 022.005.018), the Dutch Arthritis Foundation (CO-14-001, LLP-12 and LLP-22), and the European Research Council under grant agreement No. 647426 (3D-JOINT). Conceptualization by I.A.O. M.K. C.C.B. R.L. and J.M.; Methodology by I.A.O. and R.L.; Investigation by I.A.O. P.N.B. M.R. M.H.P.R. and A.M.; Formal Analysis by I.A.O. P.N.B. and M.R.; Writing of the original draft by I.A.O.; Review & Editing by I.A.O. P.N.B. R.L. and J.M.; Resources & Visualization by I.A.O. and P.N.B.; Project Administration by I.A.O.; Supervision by M.K. C.C.B. R.L. and J.M. The authors declare no competing interests. Funding Information: All tissues were obtained from biopsies of redundant tissue excised during surgery or from deceased donors who had donated their body to science, according to the guidelines of the Ethical Committee of the University Medical Center Utrecht. The authors would like to thank prof. R.L.A.W. Bleys and S. Plomp of the Department of Anatomy and Dr. L.N.A. van Adrichem of the Department of Plastic, Reconstructive and Hand Surgery of the University Medical Center Utrecht for kindly providing tissues from deceased donors and otoplasty remnants, respectively. The authors also gratefully acknowledge Iris Pennings for developing the protocol for the staining of elastin, and thank Nasim Golafshan for help with the graphical abstract. The antibody against collagen type II, developed by T.F. Linsenmayer, was obtained from the Developmental Studies Hybridoma Bank, created by the NICHD and maintained at The University of Iowa, Department of Biology, Iowa City, IA 52242, USA. The research was supported by the Netherlands Organization for Scientific Research (Graduate Program Grant 022.005.018 ), the Dutch Arthritis Foundation ( CO-14-001 , LLP-12 and LLP-22 ), and the European Research Council under grant agreement No. 647426 (3D-JOINT). Publisher Copyright: © 2022

PY - 2022/9/16

Y1 - 2022/9/16

N2 - Remaining challenges in auricular cartilage tissue engineering include acquiring sufficient amounts of regeneration-competent cells and subsequent production of high-quality neocartilage. Progenitor cells are a resident subpopulation of native cartilage, displaying a high proliferative and cartilage-forming capacity, yet their potential for regenerative medicine is vastly understudied. In this study, human auricular cartilage progenitor cells were newly identified in healthy cartilage and, importantly, in microtia-impaired chondral remnants. Their cartilage repair potential was assessed via in vitro 3D culture upon encapsulation in a gelatin-based hydrogel, and subsequent biochemical, mechanical, and histological analyses. Auricular cartilage progenitor cells demonstrate a potent ability to proliferate without losing their multipotent differentiation ability and to produce cartilage-like matrix in 3D culture. As these cells can be easily obtained through a non-deforming biopsy of the healthy ear or from the otherwise redundant microtia remnant, they can provide an important solution for long-existing challenges in auricular cartilage tissue engineering.

AB - Remaining challenges in auricular cartilage tissue engineering include acquiring sufficient amounts of regeneration-competent cells and subsequent production of high-quality neocartilage. Progenitor cells are a resident subpopulation of native cartilage, displaying a high proliferative and cartilage-forming capacity, yet their potential for regenerative medicine is vastly understudied. In this study, human auricular cartilage progenitor cells were newly identified in healthy cartilage and, importantly, in microtia-impaired chondral remnants. Their cartilage repair potential was assessed via in vitro 3D culture upon encapsulation in a gelatin-based hydrogel, and subsequent biochemical, mechanical, and histological analyses. Auricular cartilage progenitor cells demonstrate a potent ability to proliferate without losing their multipotent differentiation ability and to produce cartilage-like matrix in 3D culture. As these cells can be easily obtained through a non-deforming biopsy of the healthy ear or from the otherwise redundant microtia remnant, they can provide an important solution for long-existing challenges in auricular cartilage tissue engineering.

UR - http://www.scopus.com/inward/record.url?scp=85137411685&partnerID=8YFLogxK

U2 - 10.1016/j.isci.2022.104979

DO - 10.1016/j.isci.2022.104979

M3 - Article

C2 - 36105583

VL - 25

JO - iScience

JF - iScience

SN - 2589-0042

IS - 9

M1 - 104979

ER -

ID: 26009507