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Differential effects of short- and long-term high-fat diet feeding on hepatic fatty acid metabolism in rats. / Ciapaite, Jolita; van den Broek, Nicole M.; te Brinke, Heleen et al.

In: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, Vol. 1811, No. 7-8, 2011, p. 441-451.

Research output: Contribution to journal › Article › Academic › peer-review

Harvard

Ciapaite, J, van den Broek, NM, te Brinke, H, Nicolay, K, Jeneson, JA, Houten, SM & Prompers, JJ 2011, 'Differential effects of short- and long-term high-fat diet feeding on hepatic fatty acid metabolism in rats', BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, vol. 1811, no. 7-8, pp. 441-451. https://doi.org/10.1016/j.bbalip.2011.05.005

APA

Ciapaite, J., van den Broek, N. M., te Brinke, H., Nicolay, K., Jeneson, J. A., Houten, S. M., & Prompers, J. J. (2011). Differential effects of short- and long-term high-fat diet feeding on hepatic fatty acid metabolism in rats. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, 1811(7-8), 441-451. https://doi.org/10.1016/j.bbalip.2011.05.005

Vancouver

Ciapaite J, van den Broek NM, te Brinke H, Nicolay K, Jeneson JA, Houten SM et al. Differential effects of short- and long-term high-fat diet feeding on hepatic fatty acid metabolism in rats. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS. 2011;1811(7-8):441-451. doi: 10.1016/j.bbalip.2011.05.005

Author

Ciapaite, Jolita ; van den Broek, Nicole M. ; te Brinke, Heleen et al. / Differential effects of short- and long-term high-fat diet feeding on hepatic fatty acid metabolism in rats. In: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS. 2011 ; Vol. 1811, No. 7-8. pp. 441-451.

BibTeX

@article{9b2e3d62e20c4286968a1f4edefecfae,
title = "Differential effects of short- and long-term high-fat diet feeding on hepatic fatty acid metabolism in rats",
abstract = "Imbalance in the supply and utilization of fatty acids (FA) is thought to contribute to intrahepatic lipid (IHL) accumulation in obesity. The aim of this study was to determine the time course of changes in the liver capacity to oxidize and store FA in response to high-fat diet (HFD). Adult male Wistar rats were fed either normal chow or HFD for 2.5 weeks (short-term) and 25 weeks (long-term). Short-term HFD feeding led to a 10% higher palmitoyl-t-carnitine-driven ADP-stimulated (state 3) oxygen consumption rate in isolated liver mitochondria indicating up-regulation of 13-oxidation. This adaptation was insufficient to cope with the dietary FA overload, as indicated by accumulation of long-chain acylcarnitines, depletion of free carnitine and increase in FA content in the liver, reflecting IHL accumulation. The latter was confirmed by in vivon14 magnetic resonance spectroscopy and Oil Red 0 staining. Long-term HFD feeding caused further up-regulation of mitochondrial beta-oxidation (24% higher oxygen consumption rate in state 3 with palmitoyl-t-carnitine as substrate) and stimulation of mitochondrial biogenesis as indicated by 62% higher mitochondrial DNA copy number compared to controls. These adaptations were paralleled by a partial restoration of free carnitine levels and a decrease in long-chain acylcarnitine content. Nevertheless, there was a further increase in IHL content, accompanied by accumulation of lipid peroxidation and protein oxidation products. In conclusion, partially effective adaption of hepatic FA metabolism to long-term HFD feeding came at a price of increased oxidative stress, caused by a combination of higher FA oxidation capacity and oversupply of FA. (C) 2011 Elsevier B.V. All rights reserved",
author = "Jolita Ciapaite and {van den Broek}, {Nicole M.} and {te Brinke}, Heleen and Klaas Nicolay and Jeneson, {Jeroen A.} and Houten, {Sander M.} and Prompers, {Jeanine J.}",
year = "2011",
doi = "10.1016/j.bbalip.2011.05.005",
language = "English",
volume = "1811",
pages = "441--451",
journal = "BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS",
issn = "1388-1981",
publisher = "Elsevier",
number = "7-8",

}

RIS

TY - JOUR

T1 - Differential effects of short- and long-term high-fat diet feeding on hepatic fatty acid metabolism in rats

AU - Ciapaite, Jolita

AU - van den Broek, Nicole M.

AU - te Brinke, Heleen

AU - Nicolay, Klaas

AU - Jeneson, Jeroen A.

AU - Houten, Sander M.

AU - Prompers, Jeanine J.

PY - 2011

Y1 - 2011

N2 - Imbalance in the supply and utilization of fatty acids (FA) is thought to contribute to intrahepatic lipid (IHL) accumulation in obesity. The aim of this study was to determine the time course of changes in the liver capacity to oxidize and store FA in response to high-fat diet (HFD). Adult male Wistar rats were fed either normal chow or HFD for 2.5 weeks (short-term) and 25 weeks (long-term). Short-term HFD feeding led to a 10% higher palmitoyl-t-carnitine-driven ADP-stimulated (state 3) oxygen consumption rate in isolated liver mitochondria indicating up-regulation of 13-oxidation. This adaptation was insufficient to cope with the dietary FA overload, as indicated by accumulation of long-chain acylcarnitines, depletion of free carnitine and increase in FA content in the liver, reflecting IHL accumulation. The latter was confirmed by in vivon14 magnetic resonance spectroscopy and Oil Red 0 staining. Long-term HFD feeding caused further up-regulation of mitochondrial beta-oxidation (24% higher oxygen consumption rate in state 3 with palmitoyl-t-carnitine as substrate) and stimulation of mitochondrial biogenesis as indicated by 62% higher mitochondrial DNA copy number compared to controls. These adaptations were paralleled by a partial restoration of free carnitine levels and a decrease in long-chain acylcarnitine content. Nevertheless, there was a further increase in IHL content, accompanied by accumulation of lipid peroxidation and protein oxidation products. In conclusion, partially effective adaption of hepatic FA metabolism to long-term HFD feeding came at a price of increased oxidative stress, caused by a combination of higher FA oxidation capacity and oversupply of FA. (C) 2011 Elsevier B.V. All rights reserved

AB - Imbalance in the supply and utilization of fatty acids (FA) is thought to contribute to intrahepatic lipid (IHL) accumulation in obesity. The aim of this study was to determine the time course of changes in the liver capacity to oxidize and store FA in response to high-fat diet (HFD). Adult male Wistar rats were fed either normal chow or HFD for 2.5 weeks (short-term) and 25 weeks (long-term). Short-term HFD feeding led to a 10% higher palmitoyl-t-carnitine-driven ADP-stimulated (state 3) oxygen consumption rate in isolated liver mitochondria indicating up-regulation of 13-oxidation. This adaptation was insufficient to cope with the dietary FA overload, as indicated by accumulation of long-chain acylcarnitines, depletion of free carnitine and increase in FA content in the liver, reflecting IHL accumulation. The latter was confirmed by in vivon14 magnetic resonance spectroscopy and Oil Red 0 staining. Long-term HFD feeding caused further up-regulation of mitochondrial beta-oxidation (24% higher oxygen consumption rate in state 3 with palmitoyl-t-carnitine as substrate) and stimulation of mitochondrial biogenesis as indicated by 62% higher mitochondrial DNA copy number compared to controls. These adaptations were paralleled by a partial restoration of free carnitine levels and a decrease in long-chain acylcarnitine content. Nevertheless, there was a further increase in IHL content, accompanied by accumulation of lipid peroxidation and protein oxidation products. In conclusion, partially effective adaption of hepatic FA metabolism to long-term HFD feeding came at a price of increased oxidative stress, caused by a combination of higher FA oxidation capacity and oversupply of FA. (C) 2011 Elsevier B.V. All rights reserved

U2 - 10.1016/j.bbalip.2011.05.005

DO - 10.1016/j.bbalip.2011.05.005

M3 - Article

C2 - 21621638

VL - 1811

SP - 441

EP - 451

JO - BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

JF - BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

SN - 1388-1981

IS - 7-8

ER -

ID: 1444736