Standard

Development and evaluation of a broad bead-based multiplex immunoassay to measure IgG seroreactivity against human polyomaviruses. / Kamminga, Sergio; van der Meijden, Els; Wunderink, Herman F. et al.

In: Journal of clinical microbiology, Vol. 56, No. 4, e01566, 2018.

Research output: Contribution to journal › Article › Academic › peer-review

Harvard

Kamminga, S, van der Meijden, E, Wunderink, HF, Touzé, A, Zaaijer, HL & Feltkamp, MCW 2018, 'Development and evaluation of a broad bead-based multiplex immunoassay to measure IgG seroreactivity against human polyomaviruses', Journal of clinical microbiology, vol. 56, no. 4, e01566. https://doi.org/10.1128/JCM.01566-17

APA

Kamminga, S., van der Meijden, E., Wunderink, H. F., Touzé, A., Zaaijer, H. L., & Feltkamp, M. C. W. (2018). Development and evaluation of a broad bead-based multiplex immunoassay to measure IgG seroreactivity against human polyomaviruses. Journal of clinical microbiology, 56(4), [e01566]. https://doi.org/10.1128/JCM.01566-17

Vancouver

Kamminga S, van der Meijden E, Wunderink HF, Touzé A, Zaaijer HL, Feltkamp MCW. Development and evaluation of a broad bead-based multiplex immunoassay to measure IgG seroreactivity against human polyomaviruses. Journal of clinical microbiology. 2018;56(4):e01566. doi: 10.1128/JCM.01566-17

Author

Kamminga, Sergio ; van der Meijden, Els ; Wunderink, Herman F. et al. / Development and evaluation of a broad bead-based multiplex immunoassay to measure IgG seroreactivity against human polyomaviruses. In: Journal of clinical microbiology. 2018 ; Vol. 56, No. 4.

BibTeX

@article{428fb6b055d34eebbe88dc018f0a3ace,
title = "Development and evaluation of a broad bead-based multiplex immunoassay to measure IgG seroreactivity against human polyomaviruses",
abstract = "The family of polyomaviruses, which cause severe disease in immunocompromised hosts, has expanded substantially in recent years. To accommodate measurement of IgG seroresponses against all currently known human polyomaviruses (HPyVs), including the Lyon IARC polyomavirus (LIPyV), we extended our custom multiplex bead-based HPyV immunoassay and evaluated the performance of this pan-HPyV immunoassay. The VP1 proteins of 15 HPyVs belonging to 13 Polyomavirus species were expressed as recombinant glutathione S-transferase (GST) fusion proteins and coupled to fluorescent Luminex beads. Sera from healthy blood donors and immunocompromised kidney transplant recipients were used to analyze seroreactivity against the different HPyVs. For BK polyomavirus (BKPyV), the GST-VP1 fusion protein-directed seroresponses were compared to those obtained against BKPyV VP1 virus-like particles (VLP). Seroreactivity against most HPyVs was common and generally high in both test populations. Low seroreactivity against HPyV9, HPyV12, New Jersey PyV, and LIPyV was observed. The assay was reproducible (Pearson{\textquoteright}s r2 0.84, P 0.001) and specific. Weak but consistent cross-reactivity between the related viruses HPyV6 and HPyV7 was observed. The seroresponses measured by the GST-VP1-based immunoassay and a VP1 VLP-based enzyme-linked immunosorbent assay were highly correlated (Spearman{\textquoteright}s 0.823, P 0.001). The bead-based pan-HPyV multiplex immunoassay is a reliable tool to determine HPyV-specific seroresponses with high reproducibility and specificity and is suitable for use in seroepidemiological studies.",
author = "Sergio Kamminga and {van der Meijden}, Els and Wunderink, {Herman F.} and Antoine Touz{\'e} and Zaaijer, {Hans L.} and Feltkamp, {Mariet C. W.}",
year = "2018",
doi = "10.1128/JCM.01566-17",
language = "English",
volume = "56",
journal = "Journal of clinical microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "4",

}

RIS

TY - JOUR

T1 - Development and evaluation of a broad bead-based multiplex immunoassay to measure IgG seroreactivity against human polyomaviruses

AU - Kamminga, Sergio

AU - van der Meijden, Els

AU - Wunderink, Herman F.

AU - Touzé, Antoine

AU - Zaaijer, Hans L.

AU - Feltkamp, Mariet C. W.

PY - 2018

Y1 - 2018

N2 - The family of polyomaviruses, which cause severe disease in immunocompromised hosts, has expanded substantially in recent years. To accommodate measurement of IgG seroresponses against all currently known human polyomaviruses (HPyVs), including the Lyon IARC polyomavirus (LIPyV), we extended our custom multiplex bead-based HPyV immunoassay and evaluated the performance of this pan-HPyV immunoassay. The VP1 proteins of 15 HPyVs belonging to 13 Polyomavirus species were expressed as recombinant glutathione S-transferase (GST) fusion proteins and coupled to fluorescent Luminex beads. Sera from healthy blood donors and immunocompromised kidney transplant recipients were used to analyze seroreactivity against the different HPyVs. For BK polyomavirus (BKPyV), the GST-VP1 fusion protein-directed seroresponses were compared to those obtained against BKPyV VP1 virus-like particles (VLP). Seroreactivity against most HPyVs was common and generally high in both test populations. Low seroreactivity against HPyV9, HPyV12, New Jersey PyV, and LIPyV was observed. The assay was reproducible (Pearson’s r2 0.84, P 0.001) and specific. Weak but consistent cross-reactivity between the related viruses HPyV6 and HPyV7 was observed. The seroresponses measured by the GST-VP1-based immunoassay and a VP1 VLP-based enzyme-linked immunosorbent assay were highly correlated (Spearman’s 0.823, P 0.001). The bead-based pan-HPyV multiplex immunoassay is a reliable tool to determine HPyV-specific seroresponses with high reproducibility and specificity and is suitable for use in seroepidemiological studies.

AB - The family of polyomaviruses, which cause severe disease in immunocompromised hosts, has expanded substantially in recent years. To accommodate measurement of IgG seroresponses against all currently known human polyomaviruses (HPyVs), including the Lyon IARC polyomavirus (LIPyV), we extended our custom multiplex bead-based HPyV immunoassay and evaluated the performance of this pan-HPyV immunoassay. The VP1 proteins of 15 HPyVs belonging to 13 Polyomavirus species were expressed as recombinant glutathione S-transferase (GST) fusion proteins and coupled to fluorescent Luminex beads. Sera from healthy blood donors and immunocompromised kidney transplant recipients were used to analyze seroreactivity against the different HPyVs. For BK polyomavirus (BKPyV), the GST-VP1 fusion protein-directed seroresponses were compared to those obtained against BKPyV VP1 virus-like particles (VLP). Seroreactivity against most HPyVs was common and generally high in both test populations. Low seroreactivity against HPyV9, HPyV12, New Jersey PyV, and LIPyV was observed. The assay was reproducible (Pearson’s r2 0.84, P 0.001) and specific. Weak but consistent cross-reactivity between the related viruses HPyV6 and HPyV7 was observed. The seroresponses measured by the GST-VP1-based immunoassay and a VP1 VLP-based enzyme-linked immunosorbent assay were highly correlated (Spearman’s 0.823, P 0.001). The bead-based pan-HPyV multiplex immunoassay is a reliable tool to determine HPyV-specific seroresponses with high reproducibility and specificity and is suitable for use in seroepidemiological studies.

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85044722508&origin=inward

UR - https://www.ncbi.nlm.nih.gov/pubmed/29305551

U2 - 10.1128/JCM.01566-17

DO - 10.1128/JCM.01566-17

M3 - Article

C2 - 29305551

VL - 56

JO - Journal of clinical microbiology

JF - Journal of clinical microbiology

SN - 0095-1137

IS - 4

M1 - e01566

ER -

ID: 5677729