Standard

Detection of intrathecal antibodies to diagnose enterovirus infections of the central nervous system. / Sooksawasdi Na Ayudhya, Syriam; Sips, Gregorius J.; Bogers, Susanne et al.

In: Journal of clinical virology, Vol. 152, 105190, 01.07.2022.

Research output: Contribution to journalArticleAcademicpeer-review

Harvard

Sooksawasdi Na Ayudhya, S, Sips, GJ, Bogers, S, Leijten, LME, Laksono, BM, Smeets, LC, Bruning, A, Benschop, K, Wolthers, K, van Riel, D & GeurtsvanKessel, CH 2022, 'Detection of intrathecal antibodies to diagnose enterovirus infections of the central nervous system', Journal of clinical virology, vol. 152, 105190. https://doi.org/10.1016/j.jcv.2022.105190

APA

Sooksawasdi Na Ayudhya, S., Sips, G. J., Bogers, S., Leijten, L. M. E., Laksono, B. M., Smeets, L. C., Bruning, A., Benschop, K., Wolthers, K., van Riel, D., & GeurtsvanKessel, C. H. (2022). Detection of intrathecal antibodies to diagnose enterovirus infections of the central nervous system. Journal of clinical virology, 152, [105190]. https://doi.org/10.1016/j.jcv.2022.105190

Vancouver

Sooksawasdi Na Ayudhya S, Sips GJ, Bogers S, Leijten LME, Laksono BM, Smeets LC et al. Detection of intrathecal antibodies to diagnose enterovirus infections of the central nervous system. Journal of clinical virology. 2022 Jul 1;152:105190. doi: 10.1016/j.jcv.2022.105190

Author

Sooksawasdi Na Ayudhya, Syriam ; Sips, Gregorius J. ; Bogers, Susanne et al. / Detection of intrathecal antibodies to diagnose enterovirus infections of the central nervous system. In: Journal of clinical virology. 2022 ; Vol. 152.

BibTeX

@article{b10b537dc2b64d78b602792b9951b796,
title = "Detection of intrathecal antibodies to diagnose enterovirus infections of the central nervous system",
abstract = "Background: Enterovirus-D68 (EV-D68) predominantly causes respiratory disease. However, EV-D68 infections also have been associated with central nervous system (CNS) complications, most specifically acute flaccid myelitis (AFM). Diagnosing EV-D68-associated CNS disease is challenging since viral RNA is rarely detected in cerebrospinal fluid (CSF). Objective: In order to determine an EV antibody index (AI), we evaluated the value of a commercially available quantitative ELISA to detect EV-specific antibodies in paired CSF and blood. Study design: Nine paired CSF and blood samples were obtained from patients with EV-D68-associated AFM or from patients with a confirmed EV-associated CNS disease. EV-specific antibodies were detected using a quantitative ELISA. A Reiber diagram analysis was performed, by which the AI was calculated. Subsequently, EV ELISA results were compared with an EV-D68 virus neutralization test. Results: ELISA detected EV-specific antibodies in 1 out of the 3 patients with EV-D68-associated AFM and in 3 out of the 6 patients with confirmed EV-associated CNS disease. In these patients, the AI was indicative for intrathecal antibody production against enterovirus. Assay comparison showed that EV-D68 neutralizing antibody detection increased the sensitivity of EV-D68 antibody detection. Conclusions: A quantitative EV IgG ELISA in combination with Reiber diagram analysis and AI-calculation can be used as a diagnostic tool for EV-associated CNS disease, including EV-D68. An EV-D68 specific ELISA will improve the sensitivity of the tool. With the growing awareness that the detection of non-polio enteroviruses needs to be improved, diagnostic laboratories should consider implementation of EV serology.",
keywords = "Acute flaccid myelitis, Antibody index, Enterovirus D68, Enteroviruses, Reiber diagram, Serology",
author = "{Sooksawasdi Na Ayudhya}, Syriam and Sips, {Gregorius J.} and Susanne Bogers and Leijten, {Lonneke M. E.} and Laksono, {Brigitta M.} and Smeets, {Leonard C.} and Andrea Bruning and Kimberley Benschop and Katja Wolthers and {van Riel}, Debby and GeurtsvanKessel, {Corine H.}",
note = "Funding Information: DvR is supported by a fellowship from the Netherlands Organization for Scientific.Research (VIDI contract 91718308). Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = jul,
day = "1",
doi = "10.1016/j.jcv.2022.105190",
language = "English",
volume = "152",
journal = "Journal of clinical virology",
issn = "1386-6532",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Detection of intrathecal antibodies to diagnose enterovirus infections of the central nervous system

AU - Sooksawasdi Na Ayudhya, Syriam

AU - Sips, Gregorius J.

AU - Bogers, Susanne

AU - Leijten, Lonneke M. E.

AU - Laksono, Brigitta M.

AU - Smeets, Leonard C.

AU - Bruning, Andrea

AU - Benschop, Kimberley

AU - Wolthers, Katja

AU - van Riel, Debby

AU - GeurtsvanKessel, Corine H.

N1 - Funding Information: DvR is supported by a fellowship from the Netherlands Organization for Scientific.Research (VIDI contract 91718308). Publisher Copyright: © 2022 The Author(s)

PY - 2022/7/1

Y1 - 2022/7/1

N2 - Background: Enterovirus-D68 (EV-D68) predominantly causes respiratory disease. However, EV-D68 infections also have been associated with central nervous system (CNS) complications, most specifically acute flaccid myelitis (AFM). Diagnosing EV-D68-associated CNS disease is challenging since viral RNA is rarely detected in cerebrospinal fluid (CSF). Objective: In order to determine an EV antibody index (AI), we evaluated the value of a commercially available quantitative ELISA to detect EV-specific antibodies in paired CSF and blood. Study design: Nine paired CSF and blood samples were obtained from patients with EV-D68-associated AFM or from patients with a confirmed EV-associated CNS disease. EV-specific antibodies were detected using a quantitative ELISA. A Reiber diagram analysis was performed, by which the AI was calculated. Subsequently, EV ELISA results were compared with an EV-D68 virus neutralization test. Results: ELISA detected EV-specific antibodies in 1 out of the 3 patients with EV-D68-associated AFM and in 3 out of the 6 patients with confirmed EV-associated CNS disease. In these patients, the AI was indicative for intrathecal antibody production against enterovirus. Assay comparison showed that EV-D68 neutralizing antibody detection increased the sensitivity of EV-D68 antibody detection. Conclusions: A quantitative EV IgG ELISA in combination with Reiber diagram analysis and AI-calculation can be used as a diagnostic tool for EV-associated CNS disease, including EV-D68. An EV-D68 specific ELISA will improve the sensitivity of the tool. With the growing awareness that the detection of non-polio enteroviruses needs to be improved, diagnostic laboratories should consider implementation of EV serology.

AB - Background: Enterovirus-D68 (EV-D68) predominantly causes respiratory disease. However, EV-D68 infections also have been associated with central nervous system (CNS) complications, most specifically acute flaccid myelitis (AFM). Diagnosing EV-D68-associated CNS disease is challenging since viral RNA is rarely detected in cerebrospinal fluid (CSF). Objective: In order to determine an EV antibody index (AI), we evaluated the value of a commercially available quantitative ELISA to detect EV-specific antibodies in paired CSF and blood. Study design: Nine paired CSF and blood samples were obtained from patients with EV-D68-associated AFM or from patients with a confirmed EV-associated CNS disease. EV-specific antibodies were detected using a quantitative ELISA. A Reiber diagram analysis was performed, by which the AI was calculated. Subsequently, EV ELISA results were compared with an EV-D68 virus neutralization test. Results: ELISA detected EV-specific antibodies in 1 out of the 3 patients with EV-D68-associated AFM and in 3 out of the 6 patients with confirmed EV-associated CNS disease. In these patients, the AI was indicative for intrathecal antibody production against enterovirus. Assay comparison showed that EV-D68 neutralizing antibody detection increased the sensitivity of EV-D68 antibody detection. Conclusions: A quantitative EV IgG ELISA in combination with Reiber diagram analysis and AI-calculation can be used as a diagnostic tool for EV-associated CNS disease, including EV-D68. An EV-D68 specific ELISA will improve the sensitivity of the tool. With the growing awareness that the detection of non-polio enteroviruses needs to be improved, diagnostic laboratories should consider implementation of EV serology.

KW - Acute flaccid myelitis

KW - Antibody index

KW - Enterovirus D68

KW - Enteroviruses

KW - Reiber diagram

KW - Serology

UR - http://www.scopus.com/inward/record.url?scp=85131100778&partnerID=8YFLogxK

U2 - 10.1016/j.jcv.2022.105190

DO - 10.1016/j.jcv.2022.105190

M3 - Article

C2 - 35640402

VL - 152

JO - Journal of clinical virology

JF - Journal of clinical virology

SN - 1386-6532

M1 - 105190

ER -

ID: 25217464