Research output: Contribution to journal › Article › Academic › peer-review
Combined effect of naturally-derived biofilm inhibitors and differentiated hl-60 cells in the prevention of staphylococcus aureus biofilm formation. / Reigada, Inés; Guarch-Pérez, Clara; Patel, Jayendra Z. et al.
In: Microorganisms, Vol. 8, No. 11, 1757, 01.11.2020, p. 1-15.Research output: Contribution to journal › Article › Academic › peer-review
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TY - JOUR
T1 - Combined effect of naturally-derived biofilm inhibitors and differentiated hl-60 cells in the prevention of staphylococcus aureus biofilm formation
AU - Reigada, Inés
AU - Guarch-Pérez, Clara
AU - Patel, Jayendra Z.
AU - Riool, Martijn
AU - Savijoki, Kirsi
AU - Yli-Kauhaluoma, Jari
AU - Zaat, Sebastian A. J.
AU - Fallarero, Adyary
PY - 2020/11/1
Y1 - 2020/11/1
N2 - Nosocomial diseases represent a huge health and economic burden. A significant portion is associated with the use of medical devices, with 80% of these infections being caused by a bacterial biofilm. The insertion of a foreign material usually elicits inflammation, which can result in hampered antimicrobial capacity of the host immunity due to the effort of immune cells being directed to degrade the material. The ineffective clearance by immune cells is a perfect opportunity for bacteria to attach and form a biofilm. In this study, we analyzed the antibiofilm capacity of three naturally derived biofilm inhibitors when combined with immune cells in order to assess their applicability in implantable titanium devices and low-density polyethylene (LDPE) endotracheal tubes. To this end, we used a system based on the coculture of HL-60 cells differentiated into polymorphonuclear leukocytes (PMNs) and Staphylococcus aureus (laboratory and clinical strains) on titanium, as well as LDPE surfaces. Out of the three inhibitors, the one coded DHA1 showed the highest potential to be incorporated into implantable devices, as it displayed a combined activity with the immune cells, preventing bacterial attachment on the titanium and LDPE. The other two inhibitors seemed to also be good candidates for incorporation into LDPE endotracheal tubes.
AB - Nosocomial diseases represent a huge health and economic burden. A significant portion is associated with the use of medical devices, with 80% of these infections being caused by a bacterial biofilm. The insertion of a foreign material usually elicits inflammation, which can result in hampered antimicrobial capacity of the host immunity due to the effort of immune cells being directed to degrade the material. The ineffective clearance by immune cells is a perfect opportunity for bacteria to attach and form a biofilm. In this study, we analyzed the antibiofilm capacity of three naturally derived biofilm inhibitors when combined with immune cells in order to assess their applicability in implantable titanium devices and low-density polyethylene (LDPE) endotracheal tubes. To this end, we used a system based on the coculture of HL-60 cells differentiated into polymorphonuclear leukocytes (PMNs) and Staphylococcus aureus (laboratory and clinical strains) on titanium, as well as LDPE surfaces. Out of the three inhibitors, the one coded DHA1 showed the highest potential to be incorporated into implantable devices, as it displayed a combined activity with the immune cells, preventing bacterial attachment on the titanium and LDPE. The other two inhibitors seemed to also be good candidates for incorporation into LDPE endotracheal tubes.
KW - Biofilm
KW - Biomaterials
KW - Endotracheal tube
KW - HL-60 cells
KW - Implantable devices
KW - Medical devices
KW - Nosocomial diseases
KW - PMNs
KW - Staphylococcus aureus
KW - Titanium
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85096144117&origin=inward
UR - https://www.ncbi.nlm.nih.gov/pubmed/33182261
U2 - 10.3390/microorganisms8111757
DO - 10.3390/microorganisms8111757
M3 - Article
C2 - 33182261
VL - 8
SP - 1
EP - 15
JO - Microorganisms
JF - Microorganisms
SN - 2076-2607
IS - 11
M1 - 1757
ER -
ID: 14234549