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CD45 is a more sensitive marker than CD3 to diagnose lymphocytic myocarditis in the endomyocardiurn. / Woudstra, Linde; Biesbroek, P. Stefan; Emmens, Reindert W. et al.

In: Human pathology, Vol. 62, 2017, p. 83-90.

Research output: Contribution to journal › Article › Academic › peer-review

Harvard

Woudstra, L, Biesbroek, PS, Emmens, RW, Heymans, S, Juffermans, LJ, van der Wal, AC, van Rossum, AC, Niessen, HWM & Krijnen, PAJ 2017, 'CD45 is a more sensitive marker than CD3 to diagnose lymphocytic myocarditis in the endomyocardiurn', Human pathology, vol. 62, pp. 83-90. https://doi.org/10.1016/j.humpath.2016.11.006

APA

Woudstra, L., Biesbroek, P. S., Emmens, R. W., Heymans, S., Juffermans, L. J., van der Wal, A. C., van Rossum, A. C., Niessen, H. W. M., & Krijnen, P. A. J. (2017). CD45 is a more sensitive marker than CD3 to diagnose lymphocytic myocarditis in the endomyocardiurn. Human pathology, 62, 83-90. https://doi.org/10.1016/j.humpath.2016.11.006

Vancouver

Woudstra L, Biesbroek PS, Emmens RW, Heymans S, Juffermans LJ, van der Wal AC et al. CD45 is a more sensitive marker than CD3 to diagnose lymphocytic myocarditis in the endomyocardiurn. Human pathology. 2017;62:83-90. Epub 2016. doi: 10.1016/j.humpath.2016.11.006

Author

Woudstra, Linde ; Biesbroek, P. Stefan ; Emmens, Reindert W. et al. / CD45 is a more sensitive marker than CD3 to diagnose lymphocytic myocarditis in the endomyocardiurn. In: Human pathology. 2017 ; Vol. 62. pp. 83-90.

BibTeX

@article{50c9c64ab81e4029833f968458ac5210,
title = "CD45 is a more sensitive marker than CD3 to diagnose lymphocytic myocarditis in the endomyocardiurn",
abstract = "To diagnose lymphocytic myocarditis (LM), immunohistopathological examination of endomyocardial biopsies (EMBs) is used with a cutoff value of at least 14 leukocytes per mm(2), composed of CD3- and CD68-positive cells. We hypothesized that a more common leukocyte marker, CD45, instead of CD3 could increase the diagnostic sensitivity. Hearts of mice with acute viral myocarditis (n = 9) and of controls (n = 7) and the EMB sampling area of the left ventricular posterior wall (LVPW) obtained from autopsied hearts of patients diagnosed with LM (n = 18) and controls (n = 6) were stained with anti-CD68, anti-CD3, and anti-CD45. When applying the threshold of at least 14 leukocytes per mm(2), 33% of the mice would be diagnosed with LM with the use of CD3(+)CD68 and 89% with the use of CD45(+)CD68. In the EMB sampling area of autopsied hearts, using the cutoff value of at least 14 leukocytes per mm(2), CD3(+)CD68 could only confirm 17% of the diagnosis of LM, whereas CD45(+)CD68 could confirm 50% of the LM cases. Moreover, we compared inflammation in the EMB sampling area of the LVPW to the remaining myocardium of the LVPW and observed a significant increase of CD45(+)CD68 cells per mm(2) in patients with LM. In conclusion, the use of the common leukocyte marker CD45 increases the sensitivity of the diagnosis of LM. Furthermore, the inflammatory infiltrate in the EMB sampling area is significantly increased compared with the remaining LVPW, indicating that the sampling area constitutes the highest chance for histological diagnosis of LM. (C) 2017 Elsevier Inc. All rights reserved",
author = "Linde Woudstra and Biesbroek, {P. Stefan} and Emmens, {Reindert W.} and Stephane Heymans and Juffermans, {Lynda J.} and {van der Wal}, {Allard C.} and {van Rossum}, {Albert C.} and Niessen, {Hans W. M.} and Krijnen, {Paul A. J.}",
year = "2017",
doi = "10.1016/j.humpath.2016.11.006",
language = "English",
volume = "62",
pages = "83--90",
journal = "Human pathology",
issn = "0046-8177",
publisher = "W.B. Saunders Ltd",

}

RIS

TY - JOUR

T1 - CD45 is a more sensitive marker than CD3 to diagnose lymphocytic myocarditis in the endomyocardiurn

AU - Woudstra, Linde

AU - Biesbroek, P. Stefan

AU - Emmens, Reindert W.

AU - Heymans, Stephane

AU - Juffermans, Lynda J.

AU - van der Wal, Allard C.

AU - van Rossum, Albert C.

AU - Niessen, Hans W. M.

AU - Krijnen, Paul A. J.

PY - 2017

Y1 - 2017

N2 - To diagnose lymphocytic myocarditis (LM), immunohistopathological examination of endomyocardial biopsies (EMBs) is used with a cutoff value of at least 14 leukocytes per mm(2), composed of CD3- and CD68-positive cells. We hypothesized that a more common leukocyte marker, CD45, instead of CD3 could increase the diagnostic sensitivity. Hearts of mice with acute viral myocarditis (n = 9) and of controls (n = 7) and the EMB sampling area of the left ventricular posterior wall (LVPW) obtained from autopsied hearts of patients diagnosed with LM (n = 18) and controls (n = 6) were stained with anti-CD68, anti-CD3, and anti-CD45. When applying the threshold of at least 14 leukocytes per mm(2), 33% of the mice would be diagnosed with LM with the use of CD3(+)CD68 and 89% with the use of CD45(+)CD68. In the EMB sampling area of autopsied hearts, using the cutoff value of at least 14 leukocytes per mm(2), CD3(+)CD68 could only confirm 17% of the diagnosis of LM, whereas CD45(+)CD68 could confirm 50% of the LM cases. Moreover, we compared inflammation in the EMB sampling area of the LVPW to the remaining myocardium of the LVPW and observed a significant increase of CD45(+)CD68 cells per mm(2) in patients with LM. In conclusion, the use of the common leukocyte marker CD45 increases the sensitivity of the diagnosis of LM. Furthermore, the inflammatory infiltrate in the EMB sampling area is significantly increased compared with the remaining LVPW, indicating that the sampling area constitutes the highest chance for histological diagnosis of LM. (C) 2017 Elsevier Inc. All rights reserved

AB - To diagnose lymphocytic myocarditis (LM), immunohistopathological examination of endomyocardial biopsies (EMBs) is used with a cutoff value of at least 14 leukocytes per mm(2), composed of CD3- and CD68-positive cells. We hypothesized that a more common leukocyte marker, CD45, instead of CD3 could increase the diagnostic sensitivity. Hearts of mice with acute viral myocarditis (n = 9) and of controls (n = 7) and the EMB sampling area of the left ventricular posterior wall (LVPW) obtained from autopsied hearts of patients diagnosed with LM (n = 18) and controls (n = 6) were stained with anti-CD68, anti-CD3, and anti-CD45. When applying the threshold of at least 14 leukocytes per mm(2), 33% of the mice would be diagnosed with LM with the use of CD3(+)CD68 and 89% with the use of CD45(+)CD68. In the EMB sampling area of autopsied hearts, using the cutoff value of at least 14 leukocytes per mm(2), CD3(+)CD68 could only confirm 17% of the diagnosis of LM, whereas CD45(+)CD68 could confirm 50% of the LM cases. Moreover, we compared inflammation in the EMB sampling area of the LVPW to the remaining myocardium of the LVPW and observed a significant increase of CD45(+)CD68 cells per mm(2) in patients with LM. In conclusion, the use of the common leukocyte marker CD45 increases the sensitivity of the diagnosis of LM. Furthermore, the inflammatory infiltrate in the EMB sampling area is significantly increased compared with the remaining LVPW, indicating that the sampling area constitutes the highest chance for histological diagnosis of LM. (C) 2017 Elsevier Inc. All rights reserved

U2 - 10.1016/j.humpath.2016.11.006

DO - 10.1016/j.humpath.2016.11.006

M3 - Article

C2 - 28025077

VL - 62

SP - 83

EP - 90

JO - Human pathology

JF - Human pathology

SN - 0046-8177

ER -

ID: 3277558