Research output: Contribution to journal › Article › Academic › peer-review
Carbamazepine Increases the Risk of Sudden Cardiac Arrest by a Reduction of the Cardiac Sodium Current. / Jia, Lixia; Eroglu, Talip E.; Wilders, Ronald et al.
In: Frontiers in cell and developmental biology, Vol. 10, 891996, 03.06.2022.Research output: Contribution to journal › Article › Academic › peer-review
}
TY - JOUR
T1 - Carbamazepine Increases the Risk of Sudden Cardiac Arrest by a Reduction of the Cardiac Sodium Current
AU - Jia, Lixia
AU - Eroglu, Talip E.
AU - Wilders, Ronald
AU - Verkerk, Arie O.
AU - Tan, Hanno L.
N1 - Funding Information: This work has received funding from the European Union’s Horizon 2020 research and innovation program under acronym ESCAPE-NET, registered under grant agreement No. 733381, and the COST Action PARQ (grant agreement No. CA19137) supported by COST (European Co-operation in Science and Technology), and Chinese Scholarship Council. Publisher Copyright: Copyright © 2022 Jia, Eroglu, Wilders, Verkerk and Tan.
PY - 2022/6/3
Y1 - 2022/6/3
N2 - Aim: To assess the risk of sudden cardiac arrest (SCA) associated with the use of carbamazepine (CBZ) and establish the possible underlying cellular electrophysiological mechanisms. Methods: The SCA risk association with CBZ was studied in general population cohorts using a case–control design (n = 5,473 SCA cases, 21,866 non-SCA controls). Effects of 1–100 µM CBZ on action potentials (APs) and individual membrane currents were determined in isolated rabbit and human cardiomyocytes using the patch clamp technique. Results: CBZ use was associated with increased risk of SCA compared with no use (adjusted odds ratio 1.90 [95% confidence interval: 1.12–3.24]). CBZ reduced the AP upstroke velocity of rabbit and human cardiomyocytes, without prominent changes in other AP parameters. The reduction occurred at ≥30 µM and was frequency-dependent with a more pronounced reduction at high stimulus frequencies. The cardiac sodium current (INa) was reduced at ≥30 μM; this was accompanied by a hyperpolarizing shift in the voltage-dependency of inactivation. The recovery from inactivation was slower, which is consistent with the more pronounced AP upstroke velocity reduction at high stimulus frequencies. The main cardiac K+ and Ca2+ currents were unaffected, except reduction of L-type Ca2+ current by 100 µM CBZ. Conclusion: CBZ use is associated with an increased risk of SCA in the general population. At concentrations of 30 µM and above, CBZ reduces AP upstroke velocity and INa in cardiomyocytes. Since the concentration of 30 µM is well within the therapeutic range (20–40 µM), we conclude that CBZ increases the risk of SCA by a reduction of the cardiac INa.
AB - Aim: To assess the risk of sudden cardiac arrest (SCA) associated with the use of carbamazepine (CBZ) and establish the possible underlying cellular electrophysiological mechanisms. Methods: The SCA risk association with CBZ was studied in general population cohorts using a case–control design (n = 5,473 SCA cases, 21,866 non-SCA controls). Effects of 1–100 µM CBZ on action potentials (APs) and individual membrane currents were determined in isolated rabbit and human cardiomyocytes using the patch clamp technique. Results: CBZ use was associated with increased risk of SCA compared with no use (adjusted odds ratio 1.90 [95% confidence interval: 1.12–3.24]). CBZ reduced the AP upstroke velocity of rabbit and human cardiomyocytes, without prominent changes in other AP parameters. The reduction occurred at ≥30 µM and was frequency-dependent with a more pronounced reduction at high stimulus frequencies. The cardiac sodium current (INa) was reduced at ≥30 μM; this was accompanied by a hyperpolarizing shift in the voltage-dependency of inactivation. The recovery from inactivation was slower, which is consistent with the more pronounced AP upstroke velocity reduction at high stimulus frequencies. The main cardiac K+ and Ca2+ currents were unaffected, except reduction of L-type Ca2+ current by 100 µM CBZ. Conclusion: CBZ use is associated with an increased risk of SCA in the general population. At concentrations of 30 µM and above, CBZ reduces AP upstroke velocity and INa in cardiomyocytes. Since the concentration of 30 µM is well within the therapeutic range (20–40 µM), we conclude that CBZ increases the risk of SCA by a reduction of the cardiac INa.
KW - action potentials
KW - anti-epileptic drugs
KW - cardiomyocytes
KW - risk association
KW - sodium current
KW - sudden cardiac arrest
UR - http://www.scopus.com/inward/record.url?scp=85132900712&partnerID=8YFLogxK
U2 - 10.3389/fcell.2022.891996
DO - 10.3389/fcell.2022.891996
M3 - Article
C2 - 35721495
VL - 10
JO - Frontiers in cell and developmental biology
JF - Frontiers in cell and developmental biology
SN - 2296-634X
M1 - 891996
ER -
ID: 25196249