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A Niclosamide-releasing hot-melt extruded catheter prevents Staphylococcus aureus experimental biomaterial-associated infection. / Vazquez-Rodriguez, Jesus Augusto; Shaqour, Bahaa; Guarch-Pérez, Clara; Choińska, Emilia; Riool, Martijn; Verleije, Bart; Beyers, Koen; Costantini, Vivian J. A.; Święszkowski, Wojciech; Zaat, Sebastian A. J.; Cos, Paul; Felici, Antonio; Ferrari, Livia.

In: Scientific reports, Vol. 12, No. 1, 12329, 01.12.2022.

Research output: Contribution to journalArticleAcademicpeer-review

Harvard

Vazquez-Rodriguez, JA, Shaqour, B, Guarch-Pérez, C, Choińska, E, Riool, M, Verleije, B, Beyers, K, Costantini, VJA, Święszkowski, W, Zaat, SAJ, Cos, P, Felici, A & Ferrari, L 2022, 'A Niclosamide-releasing hot-melt extruded catheter prevents Staphylococcus aureus experimental biomaterial-associated infection', Scientific reports, vol. 12, no. 1, 12329. https://doi.org/10.1038/s41598-022-16107-4

APA

Vazquez-Rodriguez, J. A., Shaqour, B., Guarch-Pérez, C., Choińska, E., Riool, M., Verleije, B., Beyers, K., Costantini, V. J. A., Święszkowski, W., Zaat, S. A. J., Cos, P., Felici, A., & Ferrari, L. (2022). A Niclosamide-releasing hot-melt extruded catheter prevents Staphylococcus aureus experimental biomaterial-associated infection. Scientific reports, 12(1), [12329]. https://doi.org/10.1038/s41598-022-16107-4

Vancouver

Vazquez-Rodriguez JA, Shaqour B, Guarch-Pérez C, Choińska E, Riool M, Verleije B et al. A Niclosamide-releasing hot-melt extruded catheter prevents Staphylococcus aureus experimental biomaterial-associated infection. Scientific reports. 2022 Dec 1;12(1). 12329. https://doi.org/10.1038/s41598-022-16107-4

Author

Vazquez-Rodriguez, Jesus Augusto ; Shaqour, Bahaa ; Guarch-Pérez, Clara ; Choińska, Emilia ; Riool, Martijn ; Verleije, Bart ; Beyers, Koen ; Costantini, Vivian J. A. ; Święszkowski, Wojciech ; Zaat, Sebastian A. J. ; Cos, Paul ; Felici, Antonio ; Ferrari, Livia. / A Niclosamide-releasing hot-melt extruded catheter prevents Staphylococcus aureus experimental biomaterial-associated infection. In: Scientific reports. 2022 ; Vol. 12, No. 1.

BibTeX

@article{d7fd776a868a4211945aa6a499d9bb68,
title = "A Niclosamide-releasing hot-melt extruded catheter prevents Staphylococcus aureus experimental biomaterial-associated infection",
abstract = "Biomaterial-associated infections are a major healthcare challenge as they are responsible for high disease burden in critically ill patients. In this study, we have developed drug-eluting antibacterial catheters to prevent catheter-related infections. Niclosamide (NIC), originally an antiparasitic drug, was incorporated into the polymeric matrix of thermoplastic polyurethane (TPU) via solvent casting, and catheters were fabricated using hot-melt extrusion technology. The mechanical and physicochemical properties of TPU polymers loaded with NIC were studied. NIC was released in a sustained manner from the catheters and exhibited in vitro antibacterial activity against Staphylococcus aureus and Staphylococcus epidermidis. Moreover, the antibacterial efficacy of NIC-loaded catheters was validated in an in vivo biomaterial-associated infection model using a methicillin-susceptible and methicillin-resistant strain of S. aureus. The released NIC from the produced catheters reduced bacterial colonization of the catheter as well as of the surrounding tissue. In summary, the NIC-releasing hot-melt extruded catheters prevented implant colonization and reduced the bacterial colonization of peri-catheter tissue by methicillin sensitive as well as resistant S. aureus in a biomaterial-associated infection mouse model and has good prospects for preclinical development.",
author = "Vazquez-Rodriguez, {Jesus Augusto} and Bahaa Shaqour and Clara Guarch-P{\'e}rez and Emilia Choi{\'n}ska and Martijn Riool and Bart Verleije and Koen Beyers and Costantini, {Vivian J. A.} and Wojciech {\'S}wi{\c e}szkowski and Zaat, {Sebastian A. J.} and Paul Cos and Antonio Felici and Livia Ferrari",
note = "Funding Information: This research was funded by the research project PRINT-AID, the EU Framework Programme for Research and Innovation within Horizon 2020—Marie Sk{\l}odowska-Curie Innovative Training Networks under grant agreement No. 722467. Funding Information: The authors would like to thank Prof. Christophe Vande Velde from the Intelligence in Processes, Advanced Catalysts and Solvents (iPRACS) research group, Antwerp University for allowing researchers to use the thermogravimetric analysis machine in his lab. In addition, Ana Criado and Rosella Defazio for their technical assistance in the histological experimentation (Evotec, Verona, Italy); Chiara Pignaffo and Stefano Fontana (Evotec, Verona Italy) for their contribution in the in-vivo release experiments. Similarly, Jhon Quintana and Giulio Giommarelli for their assistance with the in vivo experimentation. Additionally, Mr. Jean-Pierre Smet from the Material Science department for allowing researchers to use the tensile machine in his lab. We would also like to thank Edwin Scholl and Dr. Nicole van der Wel (Electron Microscopy Center Amsterdam (EMCA), Amsterdam UMC) for their technical assistance in the collection of the SEM images. We would also like to thank Dr. Bart{\l}omiej Wysocki and Agnieszka Chmielewska for producing the coaxial nozzle that were used to extrude the catheters. Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
month = dec,
day = "1",
doi = "10.1038/s41598-022-16107-4",
language = "English",
volume = "12",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - A Niclosamide-releasing hot-melt extruded catheter prevents Staphylococcus aureus experimental biomaterial-associated infection

AU - Vazquez-Rodriguez, Jesus Augusto

AU - Shaqour, Bahaa

AU - Guarch-Pérez, Clara

AU - Choińska, Emilia

AU - Riool, Martijn

AU - Verleije, Bart

AU - Beyers, Koen

AU - Costantini, Vivian J. A.

AU - Święszkowski, Wojciech

AU - Zaat, Sebastian A. J.

AU - Cos, Paul

AU - Felici, Antonio

AU - Ferrari, Livia

N1 - Funding Information: This research was funded by the research project PRINT-AID, the EU Framework Programme for Research and Innovation within Horizon 2020—Marie Skłodowska-Curie Innovative Training Networks under grant agreement No. 722467. Funding Information: The authors would like to thank Prof. Christophe Vande Velde from the Intelligence in Processes, Advanced Catalysts and Solvents (iPRACS) research group, Antwerp University for allowing researchers to use the thermogravimetric analysis machine in his lab. In addition, Ana Criado and Rosella Defazio for their technical assistance in the histological experimentation (Evotec, Verona, Italy); Chiara Pignaffo and Stefano Fontana (Evotec, Verona Italy) for their contribution in the in-vivo release experiments. Similarly, Jhon Quintana and Giulio Giommarelli for their assistance with the in vivo experimentation. Additionally, Mr. Jean-Pierre Smet from the Material Science department for allowing researchers to use the tensile machine in his lab. We would also like to thank Edwin Scholl and Dr. Nicole van der Wel (Electron Microscopy Center Amsterdam (EMCA), Amsterdam UMC) for their technical assistance in the collection of the SEM images. We would also like to thank Dr. Bartłomiej Wysocki and Agnieszka Chmielewska for producing the coaxial nozzle that were used to extrude the catheters. Publisher Copyright: © 2022, The Author(s).

PY - 2022/12/1

Y1 - 2022/12/1

N2 - Biomaterial-associated infections are a major healthcare challenge as they are responsible for high disease burden in critically ill patients. In this study, we have developed drug-eluting antibacterial catheters to prevent catheter-related infections. Niclosamide (NIC), originally an antiparasitic drug, was incorporated into the polymeric matrix of thermoplastic polyurethane (TPU) via solvent casting, and catheters were fabricated using hot-melt extrusion technology. The mechanical and physicochemical properties of TPU polymers loaded with NIC were studied. NIC was released in a sustained manner from the catheters and exhibited in vitro antibacterial activity against Staphylococcus aureus and Staphylococcus epidermidis. Moreover, the antibacterial efficacy of NIC-loaded catheters was validated in an in vivo biomaterial-associated infection model using a methicillin-susceptible and methicillin-resistant strain of S. aureus. The released NIC from the produced catheters reduced bacterial colonization of the catheter as well as of the surrounding tissue. In summary, the NIC-releasing hot-melt extruded catheters prevented implant colonization and reduced the bacterial colonization of peri-catheter tissue by methicillin sensitive as well as resistant S. aureus in a biomaterial-associated infection mouse model and has good prospects for preclinical development.

AB - Biomaterial-associated infections are a major healthcare challenge as they are responsible for high disease burden in critically ill patients. In this study, we have developed drug-eluting antibacterial catheters to prevent catheter-related infections. Niclosamide (NIC), originally an antiparasitic drug, was incorporated into the polymeric matrix of thermoplastic polyurethane (TPU) via solvent casting, and catheters were fabricated using hot-melt extrusion technology. The mechanical and physicochemical properties of TPU polymers loaded with NIC were studied. NIC was released in a sustained manner from the catheters and exhibited in vitro antibacterial activity against Staphylococcus aureus and Staphylococcus epidermidis. Moreover, the antibacterial efficacy of NIC-loaded catheters was validated in an in vivo biomaterial-associated infection model using a methicillin-susceptible and methicillin-resistant strain of S. aureus. The released NIC from the produced catheters reduced bacterial colonization of the catheter as well as of the surrounding tissue. In summary, the NIC-releasing hot-melt extruded catheters prevented implant colonization and reduced the bacterial colonization of peri-catheter tissue by methicillin sensitive as well as resistant S. aureus in a biomaterial-associated infection mouse model and has good prospects for preclinical development.

UR - http://www.scopus.com/inward/record.url?scp=85134410711&partnerID=8YFLogxK

U2 - 10.1038/s41598-022-16107-4

DO - 10.1038/s41598-022-16107-4

M3 - Article

C2 - 35854044

VL - 12

JO - Scientific reports

JF - Scientific reports

SN - 2045-2322

IS - 1

M1 - 12329

ER -

ID: 25195649