The research of the CV Hemodynamics & Perfusion group encompasses multidisciplinary investigations ranging from bench to bedside. Mechanistic research is carried out in areas of coronary disease and microvascular pathologies in humans. Experimental projects on vascular network analysis and regional perfusion complement and support the interpretation of the functional clinical data. Physiological measurements, image analysis and biofluid mechanics are integrated with model-based analysis in clinical and pre-clinical studies.
The projects are carried out in close collaboration with basic science and clinical departments, and with national and international as well as industrial partners.

Functional assessment of coronary epicardial and microvascular pathophysiology:
Aim: to advance diagnostic and therapeutic capabilities.
Novel approaches to analyze intracoronary hemodynamic signals obtained with sensor-equipped guide wires (Prof. dr. J.J. Piek) are developed to gain mechanistic insight into dysfunction of the coronary microcirculation in humans, associated with epicardial disease, diabetes, acute myocardial infarction, heart failure or aortic stenosis. In collaboration with King's College London, we investigate coronary hemodynamic signals.

Vascular network adaptation and regional perfusion
Aim: to understand the role of microvascular structure in perfusion distribution and its adaptation in pathophysiological conditions.
A unique 3D imaging cryomicrotome has been developed in our department (Prof. dr. ir. J. A. Spaan) and is used to visualize and quantify detailed vascular networks and segmental perfusion by 3D imaging of fluorescently labeled markers (vascular casting, microspheres, cells, etc). Focus is on coronary adaptation to acute and chronic local oxygen deficiency as induced by a stenosis, cardiomyopathies, or pressure overload. Novel analysis methods applied to hemodynamic measurements are utilized together with network models to support the interpretation of experimental and clinical data. This line of research extends to applications in urology, neurology, pathology and oncology. An NWO Veni project (Dr. J.PHM van den Wijngaard) focuses on microvascular adaptation in heart failure.

Pre-clinical investigation of neovascularization in co-localization with labeled biomarkers is carried out within the EMINENCE project supported by the Center for Translational Molecular Medicine (CTMM) and the Netherlands Heart Foundation.

We participated in the EC funded FP7 project euHeart ( which aims to develop individualized, computer-based, human heart models using patient-specific data and multi-scale modeling techniques. Much of our research is conceptually embedded in the Virtual Physiological Human initiative (

With support from a recent NWO Investment grant, we are developing a 3D Fluorescent Imaging Cryomicrotome System (3D-FICS) for high-resolution multispectral biomedical structure, functional and molecular imaging.
Effective start/end date01/06/2007 → …

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