Research interests

Spermatogenesis and Stem Cells

Fertility preservation

Testis development

 

specialisation

Research

Research interests

Spermatogenesis & Stem Cells: We aim to unravel the mechanism of spermatogonial stem cell renewal and differentiation up to sperm and use this knowledge to understand spermatogenic failure in men.

Fertility preservation: We aim to develop a clinical application of human SSC autotransplantation and in vitro spermatogenesis as a way to preserve reproductive potential for instance in young boys diagnosed with cancer.

Research interests

Ans van Pelt is Professor Translational Reproductive Medicine at Amsterdam Medical Center-location AMC. She did her PhD in the group of Prof. D.G. de Rooij at the Department of Cell Biology , University Medical Center Utrecht, Utrecht University (UU) and P.T. van der Saag at the Hubrecht Institute, The Netherlands and graduated in 1992 on ‘The Role of Retinoids in Spermatogenesis’. During this PhD period she worked on male reproduction and more specifically on spermatogonial stem cells and was the first to show that retinoids are important for the differentiation of spermatogonia. She moved with de Rooij’s lab to the Department of Endocrinology at the (UU) to study the spermatogonial stem cells in more detail using the synchronized vitamin A deficient animal models. She established an isolation method for spermatogonia and investigated several aspects of the mechanism behind self-renewal and differentiation of spermatogonial stem cells in vitro. In 2007, she moved from the UU to the Academic Medical Center of the University of Amsterdam to continue her research line on spermatogonial stem cells with a focus on human spermatogonial stem cells and future use for clinical fertility treatments. Under her supervision the first culture system to propagate human spermatogonial stem cells from adult and prepubertal human testis was established. This technique will now be further developed to a clinical application for fertility restoration in male childhood cancer survivors. Among others, she is secretary board member of the Dutch Society for Stem Cell Research (www.DSSCR.nl) and PI on ZonMW TAS 116003002 grant and was coordinator of the EU MSCA FP7-PEOPLE-2013-ITN network Growsperm (www.Growsperm.eu) and participant/beneficiary in several other EU, ZonMW, NWO and KiKa grants.

Research output

  1. The risk of TESE-induced hypogonadism: A systematic review and meta-analysis

    Research output: Contribution to journalArticleAcademicpeer-review

  2. Unraveling transcriptome dynamics in human spermatogenesis

    Research output: Contribution to journalArticleAcademicpeer-review

  3. Genetic and epigenetic stability of human spermatogonial stem cells during long-term culture

    Research output: Contribution to journalArticleAcademicpeer-review

  4. In Vitro Propagation of Human Prepubertal Spermatogonial Stem Cells

    Research output: Contribution to journalComment/Letter to the editorAcademic

  5. Human stem Leydig cells: A possible source for cell therapy in hypogonadism?

    Research output: ThesisThesis: Research University of Amsterdam, graduation University of Amsterdam

  6. Tumors widely express hundreds of embryonic germline genes

    Research output: Contribution to journalArticleAcademicpeer-review

  7. In Vitro Meiosis of Male Germline Stem Cells

    Research output: Contribution to journalArticleAcademicpeer-review

  8. Infertility-Causing Haploinsufficiency Reveals TRIM28/KAP1 Requirement in Spermatogonia

    Research output: Contribution to journalArticleAcademicpeer-review

  9. Strains matter: Success of murine in vitro spermatogenesis is dependent on genetic background

    Research output: Contribution to journalArticleAcademicpeer-review

  10. Human testis phosphoproteome reveals kinases as potential targets in spermatogenesis and testicular cancer

    Research output: Contribution to journalArticleAcademicpeer-review

  11. Characterization and population dynamics of germ cells in adult macaque testicular cultures

    Research output: Contribution to journalArticleAcademicpeer-review

  12. Simultaneous Purification of Round and Elongated Spermatids from Testis Tissue Using a FACS-Based DNA Ploidy Assay

    Research output: Contribution to journalArticleAcademicpeer-review

  13. TorsinA Is Functionally Associated with Spermatogenesis

    Research output: Contribution to journalArticleAcademicpeer-review

  14. A practical blueprint to systematically study life-long health consequences of novel medically assisted reproductive treatments

    Research output: Contribution to journalReview articleAcademicpeer-review

  15. Characterization of human mesenchymal stem cells isolated from the testis

    Research output: Contribution to journalArticleAcademicpeer-review

  16. Distinct prophase arrest mechanisms in human male meiosis

    Research output: Contribution to journalArticleAcademicpeer-review

  17. Long-term health in recipients of transplanted in vitro propagated spermatogonial stem cells

    Research output: Contribution to journalArticleAcademicpeer-review

  18. Preantral follicular atresia occurs mainly through autophagy, while antral follicles degenerate mostly through apoptosis

    Research output: Contribution to journalArticleAcademicpeer-review

  19. What is the best protocol to cryopreserve immature mouse testicular cell suspensions?

    Research output: Contribution to journalArticleAcademicpeer-review

  20. Decreased spermatogonial quantity in prepubertal boys with leukaemia treated with alkylating agents

    Research output: Contribution to journalComment/Letter to the editorAcademic

  21. Descriptive Analysis of LAP1 Distribution and That of Associated Proteins throughout Spermatogenesis

    Research output: Contribution to journalArticleAcademicpeer-review

  22. Development of the testis in pre-pubertal boys with cancer after biopsy for fertility preservation

    Research output: Contribution to journalArticleAcademicpeer-review

  23. Doxorubicin and vincristine affect undifferentiated rat spermatogonia

    Research output: Contribution to journalArticleAcademicpeer-review

  24. Emi2 Is Essential for Mouse Spermatogenesis

    Research output: Contribution to journalArticleAcademicpeer-review

  25. Artificial gametes: a systematic review of biological progress towards clinical application

    Research output: Contribution to journalReview articleAcademicpeer-review

  26. AZFc deletions do not affect the function of human spermatogonia in vitro

    Research output: Contribution to journalArticleAcademicpeer-review

  27. Prenatal undernutrition and leukocyte telomere length in late adulthood: the Dutch famine birth cohort study

    Research output: Contribution to journalArticleAcademicpeer-review

  28. Cryopreservation of testicular tissue before long-term testicular cell culture does not alter in vitro cell dynamics

    Research output: Contribution to journalArticleAcademicpeer-review

  29. Spatial and temporal expression of immunoglobulin superfamily member 1 in the rat

    Research output: Contribution to journalArticleAcademicpeer-review

  30. Stem cells in reproductive medicine: ready for the patient?

    Research output: Contribution to journalArticleAcademicpeer-review

  31. Eliminating acute lymphoblastic leukemia cells from human testicular cell cultures: a pilot study

    Research output: Contribution to journalArticleAcademicpeer-review

  32. Enrichment of spermatogonial stem cells from long-term cultured human testicular cells

    Research output: Contribution to journalArticleAcademicpeer-review

  33. Mesenchymal origin of multipotent human testis-derived stem cells in human testicular cell cultures

    Research output: Contribution to journalArticleAcademicpeer-review

  34. Assuring safety without animal testing: The case for the human testis in vitro

    Research output: Contribution to journalArticleAcademicpeer-review

  35. Restoring fertility in sterile childhood cancer survivors by autotransplanting spermatogonial stem cells: are we there yet?

    Research output: Contribution to journalReview articleAcademicpeer-review

  36. Role for rodent Smc6 in pericentromeric heterochromatin domains during spermatogonial differentiation and meiosis

    Research output: Contribution to journalArticleAcademicpeer-review

  37. Human testis-derived embryonic stem cell-like cells are not pluripotent, but possess potential of mesenchymal progenitors

    Research output: Contribution to journalArticleAcademicpeer-review

  38. Molecular control of rodent spermatogenesis

    Research output: Contribution to journalReview articleAcademicpeer-review

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